Rights statement: © 2014 Hughes, Middleton. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Comparison of the structure and function of phospholamban and the Arginine-14 deficient mutant associated with dilated cardiomyopathy
AU - Hughes, Eleri
AU - Middleton, David A.
N1 - © 2014 Hughes, Middleton. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2014/9/16
Y1 - 2014/9/16
N2 - Phospholamban (PLB) is a pentameric protein that plays an important role in regulating cardiac contractility via a reversible inhibitory association with the sarcoplasmic reticulum Ca(2+)ATPase (SERCA), the enzyme responsible for maintaining correct calcium homeostasis. Here we study the functional and biophysical characteristics of a PLB mutant associated with human dilated cardiomyopathy (DCM), with a deletion of arginine at position 14 (PLBR14 Delta). In agreement with recent findings, we find that PLBR14 Delta has a reduced inhibitory effect on SERCA compared to wild type PLB (PLBWT) when reconstituted into lipid membranes. The mutation also leads to a large reduction in the protein kinase A-catalysed phosphorylation of Ser-16 in the cytoplasmic domain of PLBR14 Delta. Measurements on SERCA co-reconstituted with an equimolar mixture of PLBWT and PLBR14 Delta (representing the lethal heterozygous state associated with DCM) indicates that the loss-of-function mutation has a dominant effect on PLBWT functionality and phosphorylation capacity, suggesting that mixed PLBWT/PLBR14 Delta pentamers are formed that have characteristics typical of the mutant protein. Structural and biophysical analysis of PLBR14 Delta indicates that the mutation perturbs slightly the helical structure of the PLB cytoplasmic domain and reduces its affinity for the phospholipid bilayer surface, thereby altering the orientation of the cytoplasmic domain relative to the wild-type protein. These results indicate that the structure and function consequences of the R14 deletion have profound effects on the regulation of SERCA which may contribute to the aetiology of DCM.
AB - Phospholamban (PLB) is a pentameric protein that plays an important role in regulating cardiac contractility via a reversible inhibitory association with the sarcoplasmic reticulum Ca(2+)ATPase (SERCA), the enzyme responsible for maintaining correct calcium homeostasis. Here we study the functional and biophysical characteristics of a PLB mutant associated with human dilated cardiomyopathy (DCM), with a deletion of arginine at position 14 (PLBR14 Delta). In agreement with recent findings, we find that PLBR14 Delta has a reduced inhibitory effect on SERCA compared to wild type PLB (PLBWT) when reconstituted into lipid membranes. The mutation also leads to a large reduction in the protein kinase A-catalysed phosphorylation of Ser-16 in the cytoplasmic domain of PLBR14 Delta. Measurements on SERCA co-reconstituted with an equimolar mixture of PLBWT and PLBR14 Delta (representing the lethal heterozygous state associated with DCM) indicates that the loss-of-function mutation has a dominant effect on PLBWT functionality and phosphorylation capacity, suggesting that mixed PLBWT/PLBR14 Delta pentamers are formed that have characteristics typical of the mutant protein. Structural and biophysical analysis of PLBR14 Delta indicates that the mutation perturbs slightly the helical structure of the PLB cytoplasmic domain and reduces its affinity for the phospholipid bilayer surface, thereby altering the orientation of the cytoplasmic domain relative to the wild-type protein. These results indicate that the structure and function consequences of the R14 deletion have profound effects on the regulation of SERCA which may contribute to the aetiology of DCM.
KW - SARCOPLASMIC-RETICULUM CA2+-ATPASE
KW - DEPENDENT PROTEIN-KINASE
KW - STATE NMR-SPECTROSCOPY
KW - MONOMERIC PHOSPHOLAMBAN
KW - CYTOPLASMIC DOMAIN
KW - CALCIUM-TRANSPORT
KW - HYBRID SOLUTION
KW - LIPID-BILAYERS
KW - HEART-FAILURE
KW - WILD-TYPE
U2 - 10.1371/journal.pone.0106746
DO - 10.1371/journal.pone.0106746
M3 - Journal article
VL - 9
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 9
M1 - 106746
ER -