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Continuous two-phase flow miniaturised bioreactor for monitoring anaerobic biocatalysis by pentaerythritol tetranitrate reductase

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Continuous two-phase flow miniaturised bioreactor for monitoring anaerobic biocatalysis by pentaerythritol tetranitrate reductase. / Mohr, Stephan; Fisher, Karl; Scrutton, Nigel S.; Goddard, Nick J.; Fielden, Peter R.

In: Lab on a Chip, Vol. 10, No. 15, 2010, p. 1929-1936.

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Mohr, Stephan ; Fisher, Karl ; Scrutton, Nigel S. ; Goddard, Nick J. ; Fielden, Peter R. / Continuous two-phase flow miniaturised bioreactor for monitoring anaerobic biocatalysis by pentaerythritol tetranitrate reductase. In: Lab on a Chip. 2010 ; Vol. 10, No. 15. pp. 1929-1936.

Bibtex

@article{0055ad9660ec413db2d8e300ffaef7b8,
title = "Continuous two-phase flow miniaturised bioreactor for monitoring anaerobic biocatalysis by pentaerythritol tetranitrate reductase",
abstract = "A novel continuous recirculating two-phase flow miniaturised bioreactor was developed for biocatalytic transformations with the enzyme pentaerythritol tetranitrate reductase using on-chip spectroscopic detection of the organic and aqueous phases. A phase separation technique is described that uses electrostatic attraction to force charged droplets to merge back into the aqueous phase and thus allow the monitoring of both reaction phases during enzymatic turnover. We report an increased rate of enzyme catalysed reduction of trans-2-(2-nitrovinyl) thiophene, which was used as a model system to demonstrate the principles of the bioreactor design, compared to conventional macroscale experiments. Additional data obtained with ketoisophorone, trans-cinnamaldehyde and 2-methylmaleimide support our findings and provide a basis for improving the chemistry of biocatalysis.",
author = "Stephan Mohr and Karl Fisher and Scrutton, {Nigel S.} and Goddard, {Nick J.} and Fielden, {Peter R.}",
year = "2010",
doi = "10.1039/c003561k",
language = "English",
volume = "10",
pages = "1929--1936",
journal = "Lab on a Chip",
issn = "1473-0197",
publisher = "ROYAL SOC CHEMISTRY",
number = "15",

}

RIS

TY - JOUR

T1 - Continuous two-phase flow miniaturised bioreactor for monitoring anaerobic biocatalysis by pentaerythritol tetranitrate reductase

AU - Mohr, Stephan

AU - Fisher, Karl

AU - Scrutton, Nigel S.

AU - Goddard, Nick J.

AU - Fielden, Peter R.

PY - 2010

Y1 - 2010

N2 - A novel continuous recirculating two-phase flow miniaturised bioreactor was developed for biocatalytic transformations with the enzyme pentaerythritol tetranitrate reductase using on-chip spectroscopic detection of the organic and aqueous phases. A phase separation technique is described that uses electrostatic attraction to force charged droplets to merge back into the aqueous phase and thus allow the monitoring of both reaction phases during enzymatic turnover. We report an increased rate of enzyme catalysed reduction of trans-2-(2-nitrovinyl) thiophene, which was used as a model system to demonstrate the principles of the bioreactor design, compared to conventional macroscale experiments. Additional data obtained with ketoisophorone, trans-cinnamaldehyde and 2-methylmaleimide support our findings and provide a basis for improving the chemistry of biocatalysis.

AB - A novel continuous recirculating two-phase flow miniaturised bioreactor was developed for biocatalytic transformations with the enzyme pentaerythritol tetranitrate reductase using on-chip spectroscopic detection of the organic and aqueous phases. A phase separation technique is described that uses electrostatic attraction to force charged droplets to merge back into the aqueous phase and thus allow the monitoring of both reaction phases during enzymatic turnover. We report an increased rate of enzyme catalysed reduction of trans-2-(2-nitrovinyl) thiophene, which was used as a model system to demonstrate the principles of the bioreactor design, compared to conventional macroscale experiments. Additional data obtained with ketoisophorone, trans-cinnamaldehyde and 2-methylmaleimide support our findings and provide a basis for improving the chemistry of biocatalysis.

UR - http://www.scopus.com/inward/record.url?scp=77954581148&partnerID=8YFLogxK

U2 - 10.1039/c003561k

DO - 10.1039/c003561k

M3 - Journal article

VL - 10

SP - 1929

EP - 1936

JO - Lab on a Chip

JF - Lab on a Chip

SN - 1473-0197

IS - 15

ER -