Controlled delivery of antimicrobial gallium ions from phosphate-based glasses

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Controlled delivery of antimicrobial gallium ions from phosphate-based glasses. / Valappil, Sabeel P.; Ready, Derren; Neel, Ensanya Ali Abou et al.
In: Acta Biomaterialia, Vol. 5, No. 4, 05.2009, p. 1198-1210.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Valappil, SP, Ready, D, Neel, EAA, Pickup, DM, O'Dell, LA, Chrzanowski, W, Pratten, J, Newport, RJ, Smith, ME, Wilson, M & Knowles, JC 2009, 'Controlled delivery of antimicrobial gallium ions from phosphate-based glasses', Acta Biomaterialia, vol. 5, no. 4, pp. 1198-1210. https://doi.org/10.1016/j.actbio.2008.09.019

APA

Valappil, S. P., Ready, D., Neel, E. A. A., Pickup, D. M., O'Dell, L. A., Chrzanowski, W., Pratten, J., Newport, R. J., Smith, M. E., Wilson, M., & Knowles, J. C. (2009). Controlled delivery of antimicrobial gallium ions from phosphate-based glasses. Acta Biomaterialia, 5(4), 1198-1210. https://doi.org/10.1016/j.actbio.2008.09.019

Vancouver

Valappil SP, Ready D, Neel EAA, Pickup DM, O'Dell LA, Chrzanowski W et al. Controlled delivery of antimicrobial gallium ions from phosphate-based glasses. Acta Biomaterialia. 2009 May;5(4):1198-1210. doi: 10.1016/j.actbio.2008.09.019

Author

Valappil, Sabeel P. ; Ready, Derren ; Neel, Ensanya Ali Abou et al. / Controlled delivery of antimicrobial gallium ions from phosphate-based glasses. In: Acta Biomaterialia. 2009 ; Vol. 5, No. 4. pp. 1198-1210.

Bibtex

@article{165ff3b61322491d896dbe77691fc036,

title = "Controlled delivery of antimicrobial gallium ions from phosphate-based glasses",

abstract = "Gallium-doped phosphate-based glasses (PBGs) have been recently shown to have antibacterial activity. However, the delivery of gallium ions from these glasses can be improved by altering the calcium ion concentration to control the degradation rate of the glasses. In the present study, the effect of increasing calcium content in novel gallium (Ga2O3)-doped PBGs on the susceptibility of Pseudomonas aeruginosa is examined. The lack of new antibiotics in development makes gallium-doped PBG potentially a highly promising new therapeutic agent. The results show that an increase in calcium content (14, 15 and 16 mol.% CaO) cause a decrease in degradation rate (17.6, 13.5 and 7.3 μg mm−2 h−1), gallium ion release and antimicrobial activity against planktonic P. aeruginosa. The most potent glass composition (containing 14 mol.% CaO) was then evaluated for its ability to prevent the growth of biofilms of P. aeruginosa. Gallium release was found to reduce biofilm growth of P. aeruginosa with a maximum effect (0.86 log10 CFU reduction compared to Ga2O3-free glasses) after 48 h. Analysis of the biofilms by confocal microscopy confirmed the anti-biofilm effect of these glasses as it showed both viable and non-viable bacteria on the glass surface. Results of the solubility and ion release studies show that this glass system is suitable for controlled delivery of Ga3+. 71Ga NMR and Ga K-edge XANES measurements indicate that the gallium is octahedrally coordinated by oxygen atoms in all samples. The results presented here suggest that PBGs may be useful in controlled drug delivery applications, to deliver gallium ions in order to prevent infections due to P. aeruginosa biofilms.",

keywords = "Glass, Drug delivery , Microbiology , Antimicrobial , Biofilm",

author = "Valappil, {Sabeel P.} and Derren Ready and Neel, {Ensanya Ali Abou} and Pickup, {David M.} and O'Dell, {Luke A.} and Wojciech Chrzanowski and Jonathan Pratten and Newport, {Robert J.} and Smith, {Mark E.} and M. Wilson and Knowles, {Jonathan C.}",

year = "2009",

month = may,

doi = "10.1016/j.actbio.2008.09.019",

language = "English",

volume = "5",

pages = "1198--1210",

journal = "Acta Biomaterialia",

issn = "1878-7568",

publisher = "Elsevier BV",

number = "4",

}

RIS

TY - JOUR

T1 - Controlled delivery of antimicrobial gallium ions from phosphate-based glasses

AU - Valappil, Sabeel P.

AU - Ready, Derren

AU - Neel, Ensanya Ali Abou

AU - Pickup, David M.

AU - O'Dell, Luke A.

AU - Chrzanowski, Wojciech

AU - Pratten, Jonathan

AU - Newport, Robert J.

AU - Smith, Mark E.

AU - Wilson, M.

AU - Knowles, Jonathan C.

PY - 2009/5

Y1 - 2009/5

N2 - Gallium-doped phosphate-based glasses (PBGs) have been recently shown to have antibacterial activity. However, the delivery of gallium ions from these glasses can be improved by altering the calcium ion concentration to control the degradation rate of the glasses. In the present study, the effect of increasing calcium content in novel gallium (Ga2O3)-doped PBGs on the susceptibility of Pseudomonas aeruginosa is examined. The lack of new antibiotics in development makes gallium-doped PBG potentially a highly promising new therapeutic agent. The results show that an increase in calcium content (14, 15 and 16 mol.% CaO) cause a decrease in degradation rate (17.6, 13.5 and 7.3 μg mm−2 h−1), gallium ion release and antimicrobial activity against planktonic P. aeruginosa. The most potent glass composition (containing 14 mol.% CaO) was then evaluated for its ability to prevent the growth of biofilms of P. aeruginosa. Gallium release was found to reduce biofilm growth of P. aeruginosa with a maximum effect (0.86 log10 CFU reduction compared to Ga2O3-free glasses) after 48 h. Analysis of the biofilms by confocal microscopy confirmed the anti-biofilm effect of these glasses as it showed both viable and non-viable bacteria on the glass surface. Results of the solubility and ion release studies show that this glass system is suitable for controlled delivery of Ga3+. 71Ga NMR and Ga K-edge XANES measurements indicate that the gallium is octahedrally coordinated by oxygen atoms in all samples. The results presented here suggest that PBGs may be useful in controlled drug delivery applications, to deliver gallium ions in order to prevent infections due to P. aeruginosa biofilms.

AB - Gallium-doped phosphate-based glasses (PBGs) have been recently shown to have antibacterial activity. However, the delivery of gallium ions from these glasses can be improved by altering the calcium ion concentration to control the degradation rate of the glasses. In the present study, the effect of increasing calcium content in novel gallium (Ga2O3)-doped PBGs on the susceptibility of Pseudomonas aeruginosa is examined. The lack of new antibiotics in development makes gallium-doped PBG potentially a highly promising new therapeutic agent. The results show that an increase in calcium content (14, 15 and 16 mol.% CaO) cause a decrease in degradation rate (17.6, 13.5 and 7.3 μg mm−2 h−1), gallium ion release and antimicrobial activity against planktonic P. aeruginosa. The most potent glass composition (containing 14 mol.% CaO) was then evaluated for its ability to prevent the growth of biofilms of P. aeruginosa. Gallium release was found to reduce biofilm growth of P. aeruginosa with a maximum effect (0.86 log10 CFU reduction compared to Ga2O3-free glasses) after 48 h. Analysis of the biofilms by confocal microscopy confirmed the anti-biofilm effect of these glasses as it showed both viable and non-viable bacteria on the glass surface. Results of the solubility and ion release studies show that this glass system is suitable for controlled delivery of Ga3+. 71Ga NMR and Ga K-edge XANES measurements indicate that the gallium is octahedrally coordinated by oxygen atoms in all samples. The results presented here suggest that PBGs may be useful in controlled drug delivery applications, to deliver gallium ions in order to prevent infections due to P. aeruginosa biofilms.

KW - Glass

KW - Drug delivery

KW - Microbiology

KW - Antimicrobial

KW - Biofilm

U2 - 10.1016/j.actbio.2008.09.019

DO - 10.1016/j.actbio.2008.09.019

M3 - Journal article

VL - 5

SP - 1198

EP - 1210

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1878-7568

IS - 4

ER -

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