Conversion of D-hamamelose into 2-carboxy-D-arabinitol and 2-carboxy-D- arabinitol 1-phosphate in leaves of Phaseolus vulgaris L

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Conversion of D-hamamelose into 2-carboxy-D-arabinitol and 2-carboxy-D- arabinitol 1-phosphate in leaves of Phaseolus vulgaris L. / John Andralojc, P.; Keys, Alfred J.; Martindale, Wayne et al.
In: Journal of Biological Chemistry, Vol. 271, No. 43, 06.11.1996, p. 26803-26809.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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John Andralojc, P. ; Keys, Alfred J. ; Martindale, Wayne et al. / Conversion of D-hamamelose into 2-carboxy-D-arabinitol and 2-carboxy-D- arabinitol 1-phosphate in leaves of Phaseolus vulgaris L. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 43. pp. 26803-26809.

Bibtex

@article{b11fdf2f52774b8bb7c311a9b689b896,

title = "Conversion of D-hamamelose into 2-carboxy-D-arabinitol and 2-carboxy-D- arabinitol 1-phosphate in leaves of Phaseolus vulgaris L",

abstract = "[1-14C]Hamamelose (2-hydroxymethyl-D-ribose) was synthesized by reaction of ribulose 5-phosphate with potassium [14C]cyanide, catalytic hydrogenation of the resulting cyanohydrin, and dephosphorylation of the product. Its identity was established by a chromatographic comparison with hamamelose isolated from the bark of witch hazel (Hamamelis virginiana L.). Following vacuum infiltration of the [1-14C]hamamelose into leaf discs from Phaseolus vulgaris L., 14C-labeled 2-carboxy-D-arabinitol (CA) and 2- carboxy-D-arabinitol 1-phosphate (CA1P) were formed, in the dark. Conversion of hamamelose to both CA and CA1P in the leaf discs was inhibited by dithiothreitol and sodium fluoride, although at high concentrations of these inhibitors conversion into CA was still evident when conversion into CA1P was totally inhibited. Wheat (Triticum aestivum L.) leaves converted hamamelose into CA without formation of CA1P. Leaves from P. vulgaris contained 68 nmol · g-1 fresh weight of hamamelose in the light and 35 nmol · g-1 fresh weight in the dark. A pathway for the biosynthesis of CA1P from Calvin cycle intermediates is proposed which includes the sequence: hamamelose CA → CA1P.",

author = "{John Andralojc}, P. and Keys, {Alfred J.} and Wayne Martindale and Dawson, {Glenn W.} and Parry, {Martin A J}",

year = "1996",

month = nov,

day = "6",

language = "English",

volume = "271",

pages = "26803--26809",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology Inc.",

number = "43",

}

RIS

TY - JOUR

T1 - Conversion of D-hamamelose into 2-carboxy-D-arabinitol and 2-carboxy-D- arabinitol 1-phosphate in leaves of Phaseolus vulgaris L

AU - John Andralojc, P.

AU - Keys, Alfred J.

AU - Martindale, Wayne

AU - Dawson, Glenn W.

AU - Parry, Martin A J

PY - 1996/11/6

Y1 - 1996/11/6

N2 - [1-14C]Hamamelose (2-hydroxymethyl-D-ribose) was synthesized by reaction of ribulose 5-phosphate with potassium [14C]cyanide, catalytic hydrogenation of the resulting cyanohydrin, and dephosphorylation of the product. Its identity was established by a chromatographic comparison with hamamelose isolated from the bark of witch hazel (Hamamelis virginiana L.). Following vacuum infiltration of the [1-14C]hamamelose into leaf discs from Phaseolus vulgaris L., 14C-labeled 2-carboxy-D-arabinitol (CA) and 2- carboxy-D-arabinitol 1-phosphate (CA1P) were formed, in the dark. Conversion of hamamelose to both CA and CA1P in the leaf discs was inhibited by dithiothreitol and sodium fluoride, although at high concentrations of these inhibitors conversion into CA was still evident when conversion into CA1P was totally inhibited. Wheat (Triticum aestivum L.) leaves converted hamamelose into CA without formation of CA1P. Leaves from P. vulgaris contained 68 nmol · g-1 fresh weight of hamamelose in the light and 35 nmol · g-1 fresh weight in the dark. A pathway for the biosynthesis of CA1P from Calvin cycle intermediates is proposed which includes the sequence: hamamelose CA → CA1P.

AB - [1-14C]Hamamelose (2-hydroxymethyl-D-ribose) was synthesized by reaction of ribulose 5-phosphate with potassium [14C]cyanide, catalytic hydrogenation of the resulting cyanohydrin, and dephosphorylation of the product. Its identity was established by a chromatographic comparison with hamamelose isolated from the bark of witch hazel (Hamamelis virginiana L.). Following vacuum infiltration of the [1-14C]hamamelose into leaf discs from Phaseolus vulgaris L., 14C-labeled 2-carboxy-D-arabinitol (CA) and 2- carboxy-D-arabinitol 1-phosphate (CA1P) were formed, in the dark. Conversion of hamamelose to both CA and CA1P in the leaf discs was inhibited by dithiothreitol and sodium fluoride, although at high concentrations of these inhibitors conversion into CA was still evident when conversion into CA1P was totally inhibited. Wheat (Triticum aestivum L.) leaves converted hamamelose into CA without formation of CA1P. Leaves from P. vulgaris contained 68 nmol · g-1 fresh weight of hamamelose in the light and 35 nmol · g-1 fresh weight in the dark. A pathway for the biosynthesis of CA1P from Calvin cycle intermediates is proposed which includes the sequence: hamamelose CA → CA1P.

UR - http://www.scopus.com/inward/record.url?scp=10244252723&partnerID=8YFLogxK

M3 - Journal article

C2 - 8900161

AN - SCOPUS:10244252723

VL - 271

SP - 26803

EP - 26809

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 43

ER -

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