Culture of dermal fibroblasts and protein adsorption on block conetworks of poly(butyl methacrylate-block-(2,3 propandiol-1-methacrylate-stat-ethandiol dimethacrylate))

Biomedical and Life Sciences

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https://doi.org/10.1016/j.biomaterials.2006.09.024
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Keywords

amphiphilic conetwork, protein adsorption, cell culture, fibroblast, BABOON FIBRINOGEN ADSORPTION, AMPHIPHILIC NETWORKS, EXTRACELLULAR-MATRIX, ADHESION, FIBRONECTIN, SCAFFOLDS, HYDROGEL, PROLIFERATION, ARCHITECTURE, MEMBRANES

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Culture of dermal fibroblasts and protein adsorption on block conetworks of poly(butyl methacrylate-block-(2,3 propandiol-1-methacrylate-stat-ethandiol dimethacrylate)). / Sun, Yun; Collett, Jon; Fullwood, Nigel J. et al.
In: Biomaterials, Vol. 28, No. 4, 02.2007, p. 661-670.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{02ffdf3e7662451fb8fecae03c0ff4e9,

title = "Culture of dermal fibroblasts and protein adsorption on block conetworks of poly(butyl methacrylate-block-(2,3 propandiol-1-methacrylate-stat-ethandiol dimethacrylate))",

abstract = "Amphiphilic block terpolymer conetworks composed of butyl methacrylate (BMA), 2,3 propandiol-l-methacrylate (GMMA) and ethandiol dimethacrylate (EDMA) were synthesized. Telechelic oligomers with the carboxylic acid end groups were made via ozonolysis of poly(BMA-co-butadiene) and then these were reacted with glycidyl methacrylate to obtain cross-linkable vinyl groups at both chain ends. Networks were then formed via free radical copolymerization with EDMA and GMMA or 2-methyl-acrylic acid 2,2-dimethyl[1,3]dioxolan-4-ylmethyl ester (GMAc). The acetonide groups of the GMAc units were then removed, by reaction with selenium dioxide and hydrogen peroxide, to give networks with the same molecular structure as the GMMA terpolymers but different cell adhesion and protein adsorption properties. Protein adsorption was maximised in networks prepared with GMMA rather than with GMAc followed by removal of the acetonide. Block conetworks that were synthesised with GMAc were poor substrates for cell proliferation whilst the GMMA class support good levels of both cell viability and proliferation. It is suggested that the difference in behaviour is derived from changes in the surface composition. (c) 2006 Elsevier Ltd. All rights reserved.",

keywords = "amphiphilic conetwork, protein adsorption, cell culture, fibroblast, BABOON FIBRINOGEN ADSORPTION, AMPHIPHILIC NETWORKS, EXTRACELLULAR-MATRIX, ADHESION, FIBRONECTIN, SCAFFOLDS, HYDROGEL, PROLIFERATION, ARCHITECTURE, MEMBRANES",

author = "Yun Sun and Jon Collett and Fullwood, {Nigel J.} and {Mac Neil}, Sheila and S. Rimmer",

year = "2007",

month = feb,

doi = "10.1016/j.biomaterials.2006.09.024",

language = "English",

volume = "28",

pages = "661--670",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "PERGAMON-ELSEVIER SCIENCE LTD",

number = "4",

}

RIS

TY - JOUR

T1 - Culture of dermal fibroblasts and protein adsorption on block conetworks of poly(butyl methacrylate-block-(2,3 propandiol-1-methacrylate-stat-ethandiol dimethacrylate))

AU - Sun, Yun

AU - Collett, Jon

AU - Fullwood, Nigel J.

AU - Mac Neil, Sheila

AU - Rimmer, S.

PY - 2007/2

Y1 - 2007/2

N2 - Amphiphilic block terpolymer conetworks composed of butyl methacrylate (BMA), 2,3 propandiol-l-methacrylate (GMMA) and ethandiol dimethacrylate (EDMA) were synthesized. Telechelic oligomers with the carboxylic acid end groups were made via ozonolysis of poly(BMA-co-butadiene) and then these were reacted with glycidyl methacrylate to obtain cross-linkable vinyl groups at both chain ends. Networks were then formed via free radical copolymerization with EDMA and GMMA or 2-methyl-acrylic acid 2,2-dimethyl[1,3]dioxolan-4-ylmethyl ester (GMAc). The acetonide groups of the GMAc units were then removed, by reaction with selenium dioxide and hydrogen peroxide, to give networks with the same molecular structure as the GMMA terpolymers but different cell adhesion and protein adsorption properties. Protein adsorption was maximised in networks prepared with GMMA rather than with GMAc followed by removal of the acetonide. Block conetworks that were synthesised with GMAc were poor substrates for cell proliferation whilst the GMMA class support good levels of both cell viability and proliferation. It is suggested that the difference in behaviour is derived from changes in the surface composition. (c) 2006 Elsevier Ltd. All rights reserved.

AB - Amphiphilic block terpolymer conetworks composed of butyl methacrylate (BMA), 2,3 propandiol-l-methacrylate (GMMA) and ethandiol dimethacrylate (EDMA) were synthesized. Telechelic oligomers with the carboxylic acid end groups were made via ozonolysis of poly(BMA-co-butadiene) and then these were reacted with glycidyl methacrylate to obtain cross-linkable vinyl groups at both chain ends. Networks were then formed via free radical copolymerization with EDMA and GMMA or 2-methyl-acrylic acid 2,2-dimethyl[1,3]dioxolan-4-ylmethyl ester (GMAc). The acetonide groups of the GMAc units were then removed, by reaction with selenium dioxide and hydrogen peroxide, to give networks with the same molecular structure as the GMMA terpolymers but different cell adhesion and protein adsorption properties. Protein adsorption was maximised in networks prepared with GMMA rather than with GMAc followed by removal of the acetonide. Block conetworks that were synthesised with GMAc were poor substrates for cell proliferation whilst the GMMA class support good levels of both cell viability and proliferation. It is suggested that the difference in behaviour is derived from changes in the surface composition. (c) 2006 Elsevier Ltd. All rights reserved.

KW - amphiphilic conetwork

KW - protein adsorption

KW - cell culture

KW - fibroblast

KW - BABOON FIBRINOGEN ADSORPTION

KW - AMPHIPHILIC NETWORKS

KW - EXTRACELLULAR-MATRIX

KW - ADHESION

KW - FIBRONECTIN

KW - SCAFFOLDS

KW - HYDROGEL

KW - PROLIFERATION

KW - ARCHITECTURE

KW - MEMBRANES

U2 - 10.1016/j.biomaterials.2006.09.024

DO - 10.1016/j.biomaterials.2006.09.024

M3 - Journal article

VL - 28

SP - 661

EP - 670

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 4

ER -

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