Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Cuticular permeance in relation to wax and cutin development along the growing barley ( Hordeum vulgare ) leaf.
AU - Richardson, Andrew
AU - Wojciechowski, Tobias
AU - Franke, Rochus
AU - Schreiber, Lukas
AU - Kerstiens, Gerhard
AU - Jarvis, Mike
AU - Fricke, Wieland
PY - 2007/5
Y1 - 2007/5
N2 - The developing leaf three of barley provides an excellent model system for the direct determination of relationships between amounts of waxes and cutin and cuticular permeance. Permeance of the cuticle was assessed via the time-course of uptake of either toluidine blue or 14C-labelled benzoic acid ([14C] BA) along the length of the developing leaf. Toluidine blue uptake only occurred within the region 0–25 mm from the point of leaf insertion (POLI). Resistance—the inverse of permeance—to uptake of [14C] BA was determined for four leaf regions and was lowest in the region 10–20 mm above POLI. At 20–30 and 50–60 mm above POLI, it increased by factors of 6 and a further 32, respectively. Above the point of emergence of leaf three from the sheath of leaf two, which was 76–80 mm above POLI, resistance was as high as at 50–60 mm above POLI. GC-FID/MS analyses of wax and cutin showed that: (1) the initial seven fold increase in cuticular resistance coincided with increase in cutin coverage and appearance of waxes; (2) the second, larger and final increase in cuticle resistance was accompanied by an increase in wax coverage, whereas cutin coverage remained unchanged; (3) cutin deposition in barley leaf epidermis occurred in parallel with cell elongation, whereas deposition of significant amounts of wax commenced as cells ceased to elongate.
AB - The developing leaf three of barley provides an excellent model system for the direct determination of relationships between amounts of waxes and cutin and cuticular permeance. Permeance of the cuticle was assessed via the time-course of uptake of either toluidine blue or 14C-labelled benzoic acid ([14C] BA) along the length of the developing leaf. Toluidine blue uptake only occurred within the region 0–25 mm from the point of leaf insertion (POLI). Resistance—the inverse of permeance—to uptake of [14C] BA was determined for four leaf regions and was lowest in the region 10–20 mm above POLI. At 20–30 and 50–60 mm above POLI, it increased by factors of 6 and a further 32, respectively. Above the point of emergence of leaf three from the sheath of leaf two, which was 76–80 mm above POLI, resistance was as high as at 50–60 mm above POLI. GC-FID/MS analyses of wax and cutin showed that: (1) the initial seven fold increase in cuticular resistance coincided with increase in cutin coverage and appearance of waxes; (2) the second, larger and final increase in cuticle resistance was accompanied by an increase in wax coverage, whereas cutin coverage remained unchanged; (3) cutin deposition in barley leaf epidermis occurred in parallel with cell elongation, whereas deposition of significant amounts of wax commenced as cells ceased to elongate.
KW - cuticular permeance
KW - cutin
KW - epidermal cell development
KW - Hordeum
KW - leaf growth
KW - transpirational water loss
KW - wax
KW - CLIVIA-MINIATA REG
KW - PRUNUS LAUROCERASUS L
KW - PLANT CUTICLES
KW - WATER PERMEABILITY
KW - BARRIER PROPERTIES
KW - FINE-STRUCTURE
KW - LEAVES
KW - ARABIDOPSIS
KW - TRANSPIRATION
KW - ELONGATION
U2 - 10.1007/s00425-006-0456-0
DO - 10.1007/s00425-006-0456-0
M3 - Journal article
VL - 225
SP - 1471
EP - 1481
JO - Planta
JF - Planta
SN - 0032-0935
IS - 6
ER -