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Cuticular permeance in relation to wax and cutin development along the growing barley ( Hordeum vulgare ) leaf.

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Cuticular permeance in relation to wax and cutin development along the growing barley ( Hordeum vulgare ) leaf. / Richardson, Andrew; Wojciechowski, Tobias; Franke, Rochus; Schreiber, Lukas; Kerstiens, Gerhard; Jarvis, Mike; Fricke, Wieland.

In: Planta, Vol. 225, No. 6, 05.2007, p. 1471-1481.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Richardson, A, Wojciechowski, T, Franke, R, Schreiber, L, Kerstiens, G, Jarvis, M & Fricke, W 2007, 'Cuticular permeance in relation to wax and cutin development along the growing barley ( Hordeum vulgare ) leaf.', Planta, vol. 225, no. 6, pp. 1471-1481. https://doi.org/10.1007/s00425-006-0456-0

APA

Richardson, A., Wojciechowski, T., Franke, R., Schreiber, L., Kerstiens, G., Jarvis, M., & Fricke, W. (2007). Cuticular permeance in relation to wax and cutin development along the growing barley ( Hordeum vulgare ) leaf. Planta, 225(6), 1471-1481. https://doi.org/10.1007/s00425-006-0456-0

Vancouver

Author

Richardson, Andrew ; Wojciechowski, Tobias ; Franke, Rochus ; Schreiber, Lukas ; Kerstiens, Gerhard ; Jarvis, Mike ; Fricke, Wieland. / Cuticular permeance in relation to wax and cutin development along the growing barley ( Hordeum vulgare ) leaf. In: Planta. 2007 ; Vol. 225, No. 6. pp. 1471-1481.

Bibtex

@article{952c3f2152374d9ba7efb755c31e916a,
title = "Cuticular permeance in relation to wax and cutin development along the growing barley ( Hordeum vulgare ) leaf.",
abstract = "The developing leaf three of barley provides an excellent model system for the direct determination of relationships between amounts of waxes and cutin and cuticular permeance. Permeance of the cuticle was assessed via the time-course of uptake of either toluidine blue or 14C-labelled benzoic acid ([14C] BA) along the length of the developing leaf. Toluidine blue uptake only occurred within the region 0–25 mm from the point of leaf insertion (POLI). Resistance—the inverse of permeance—to uptake of [14C] BA was determined for four leaf regions and was lowest in the region 10–20 mm above POLI. At 20–30 and 50–60 mm above POLI, it increased by factors of 6 and a further 32, respectively. Above the point of emergence of leaf three from the sheath of leaf two, which was 76–80 mm above POLI, resistance was as high as at 50–60 mm above POLI. GC-FID/MS analyses of wax and cutin showed that: (1) the initial seven fold increase in cuticular resistance coincided with increase in cutin coverage and appearance of waxes; (2) the second, larger and final increase in cuticle resistance was accompanied by an increase in wax coverage, whereas cutin coverage remained unchanged; (3) cutin deposition in barley leaf epidermis occurred in parallel with cell elongation, whereas deposition of significant amounts of wax commenced as cells ceased to elongate.",
keywords = "cuticular permeance, cutin, epidermal cell development, Hordeum, leaf growth, transpirational water loss, wax, CLIVIA-MINIATA REG, PRUNUS LAUROCERASUS L, PLANT CUTICLES, WATER PERMEABILITY, BARRIER PROPERTIES, FINE-STRUCTURE, LEAVES, ARABIDOPSIS, TRANSPIRATION, ELONGATION",
author = "Andrew Richardson and Tobias Wojciechowski and Rochus Franke and Lukas Schreiber and Gerhard Kerstiens and Mike Jarvis and Wieland Fricke",
year = "2007",
month = may,
doi = "10.1007/s00425-006-0456-0",
language = "English",
volume = "225",
pages = "1471--1481",
journal = "Planta",
issn = "0032-0935",
publisher = "Springer",
number = "6",

}

RIS

TY - JOUR

T1 - Cuticular permeance in relation to wax and cutin development along the growing barley ( Hordeum vulgare ) leaf.

AU - Richardson, Andrew

AU - Wojciechowski, Tobias

AU - Franke, Rochus

AU - Schreiber, Lukas

AU - Kerstiens, Gerhard

AU - Jarvis, Mike

AU - Fricke, Wieland

PY - 2007/5

Y1 - 2007/5

N2 - The developing leaf three of barley provides an excellent model system for the direct determination of relationships between amounts of waxes and cutin and cuticular permeance. Permeance of the cuticle was assessed via the time-course of uptake of either toluidine blue or 14C-labelled benzoic acid ([14C] BA) along the length of the developing leaf. Toluidine blue uptake only occurred within the region 0–25 mm from the point of leaf insertion (POLI). Resistance—the inverse of permeance—to uptake of [14C] BA was determined for four leaf regions and was lowest in the region 10–20 mm above POLI. At 20–30 and 50–60 mm above POLI, it increased by factors of 6 and a further 32, respectively. Above the point of emergence of leaf three from the sheath of leaf two, which was 76–80 mm above POLI, resistance was as high as at 50–60 mm above POLI. GC-FID/MS analyses of wax and cutin showed that: (1) the initial seven fold increase in cuticular resistance coincided with increase in cutin coverage and appearance of waxes; (2) the second, larger and final increase in cuticle resistance was accompanied by an increase in wax coverage, whereas cutin coverage remained unchanged; (3) cutin deposition in barley leaf epidermis occurred in parallel with cell elongation, whereas deposition of significant amounts of wax commenced as cells ceased to elongate.

AB - The developing leaf three of barley provides an excellent model system for the direct determination of relationships between amounts of waxes and cutin and cuticular permeance. Permeance of the cuticle was assessed via the time-course of uptake of either toluidine blue or 14C-labelled benzoic acid ([14C] BA) along the length of the developing leaf. Toluidine blue uptake only occurred within the region 0–25 mm from the point of leaf insertion (POLI). Resistance—the inverse of permeance—to uptake of [14C] BA was determined for four leaf regions and was lowest in the region 10–20 mm above POLI. At 20–30 and 50–60 mm above POLI, it increased by factors of 6 and a further 32, respectively. Above the point of emergence of leaf three from the sheath of leaf two, which was 76–80 mm above POLI, resistance was as high as at 50–60 mm above POLI. GC-FID/MS analyses of wax and cutin showed that: (1) the initial seven fold increase in cuticular resistance coincided with increase in cutin coverage and appearance of waxes; (2) the second, larger and final increase in cuticle resistance was accompanied by an increase in wax coverage, whereas cutin coverage remained unchanged; (3) cutin deposition in barley leaf epidermis occurred in parallel with cell elongation, whereas deposition of significant amounts of wax commenced as cells ceased to elongate.

KW - cuticular permeance

KW - cutin

KW - epidermal cell development

KW - Hordeum

KW - leaf growth

KW - transpirational water loss

KW - wax

KW - CLIVIA-MINIATA REG

KW - PRUNUS LAUROCERASUS L

KW - PLANT CUTICLES

KW - WATER PERMEABILITY

KW - BARRIER PROPERTIES

KW - FINE-STRUCTURE

KW - LEAVES

KW - ARABIDOPSIS

KW - TRANSPIRATION

KW - ELONGATION

U2 - 10.1007/s00425-006-0456-0

DO - 10.1007/s00425-006-0456-0

M3 - Journal article

VL - 225

SP - 1471

EP - 1481

JO - Planta

JF - Planta

SN - 0032-0935

IS - 6

ER -