Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Cytokinesis in the heliozoan Actinophrys sol
AU - Ockleford, C D
PY - 1974/12
Y1 - 1974/12
N2 - A study of cytokinesis in the heliozoan Actinophrys sol has been made using low-temperature treatments, antimitotic drugs, cytochalasin B, light microscopy and electron microscopy. It reveals that microtubular axopodia remain extended during cell division and play a major role in it.Data indicate that when organisms are attached to the bases of culture dishes the normal locomotive mechanism of the presumptive daughter cells pulls them apart. However, when Actinophrys are floating freely in their culture medium, they are still able to undertake division. In this situation interactions between axopodia from opposite daughter cell bodies appear to facilitate the movement apart of the prospective daughter cells.The present study and other published accounts indicate that a type of cytokinesis exists which is not explicable in terms of the ‘contractile ring’ or ‘fusing vesicle’ theories. To account for these observations a ‘barge pole’ model of division is suggested.
AB - A study of cytokinesis in the heliozoan Actinophrys sol has been made using low-temperature treatments, antimitotic drugs, cytochalasin B, light microscopy and electron microscopy. It reveals that microtubular axopodia remain extended during cell division and play a major role in it.Data indicate that when organisms are attached to the bases of culture dishes the normal locomotive mechanism of the presumptive daughter cells pulls them apart. However, when Actinophrys are floating freely in their culture medium, they are still able to undertake division. In this situation interactions between axopodia from opposite daughter cell bodies appear to facilitate the movement apart of the prospective daughter cells.The present study and other published accounts indicate that a type of cytokinesis exists which is not explicable in terms of the ‘contractile ring’ or ‘fusing vesicle’ theories. To account for these observations a ‘barge pole’ model of division is suggested.
KW - Animals
KW - Cell Division/drug effects
KW - Cell Movement
KW - Cell Nucleus
KW - Colchicine/pharmacology
KW - Cytochalasin B/pharmacology
KW - Cytoplasm/ultrastructure
KW - Cytoplasmic Streaming/drug effects
KW - Dimethyl Sulfoxide/pharmacology
KW - Eukaryota/cytology
KW - Inclusion Bodies/ultrastructure
KW - Microscopy, Electron
KW - Microscopy, Phase-Contrast
KW - Microtubules/physiology
KW - Models, Biological
KW - Pseudopodia
KW - Temperature
M3 - Journal article
C2 - 4375157
VL - 16
SP - 499
EP - 517
JO - Journal of Cell Science
JF - Journal of Cell Science
SN - 0021-9533
IS - 3
ER -