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Cytoplasmic calcium and Fucus egg activation

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Cytoplasmic calcium and Fucus egg activation. / Roberts, Stephen; Gillot, I.; Brownlee, Colin.

In: Development, Vol. 120, No. 1, 01.1994, p. 155-163.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

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Roberts, S, Gillot, I & Brownlee, C 1994, 'Cytoplasmic calcium and Fucus egg activation', Development, vol. 120, no. 1, pp. 155-163. <http://dev.biologists.org/content/120/1/155.abstract>

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Roberts S, Gillot I, Brownlee C. Cytoplasmic calcium and Fucus egg activation. Development. 1994 Jan;120(1):155-163.

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Roberts, Stephen ; Gillot, I. ; Brownlee, Colin. / Cytoplasmic calcium and Fucus egg activation. In: Development. 1994 ; Vol. 120, No. 1. pp. 155-163.

Bibtex

@article{cdc6c4bd9d6041b2be17ce64172dee8f,
title = "Cytoplasmic calcium and Fucus egg activation",
abstract = "Eggs of the marine brown alga, Fucus serratus, exhibit small transient elevations of cytosolic Ca2+ of variable magnitude, corresponding to the onset of the fertilization potential. Microinjection of Ca2+ buffers (BAPTA (1-2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid)) at concentrations sufficient to block any global fertilization-associated Ca-cyt(2+) elevation did not inhibit egg activation (monitored as exocytosis of cell wall) or subsequent development. However, egg activation could be inhibited with higher buffer concentrations. Br(2)BAPTA (K-d = 1.6 mu M) was a more effective inhibitor of egg activation than BAPTA (K-d = 0.17 mu M). Localized microinjection of Ca2+ produced only localized cell wall exocytosis at the injection site. Eggs injected with Br(2)BAPTA at intracellular concentrations, which blocked egg activation, exhibited prolonged fertilization potentials. Ca-45(2+) influx across the plasma membrane increased during fertilization. Our results show that a large transient global elevation of Ca-cyt(2+) is not necessary for Fucus egg activation but rather a localized elevation to micromolar levels results, at least in part, from increased Ca2+ influx across the plasma membrane. This is needed for early fertilization events, including the generation of the fertilization potential and cell wall secretion.",
keywords = "FUCUS, EGG ACTIVATION, CYTOPLASMIC CALCIUM, FURA-2-DEXTRAN, BAPTA, SEA-URCHIN EGGS, URECHIS-CAUPO EGGS, CORTICAL REACTION, FERTILIZATION, SPERM, INCREASE, INFLUX, WAVE, CHANNELS, EVENTS",
author = "Stephen Roberts and I. Gillot and Colin Brownlee",
year = "1994",
month = jan,
language = "English",
volume = "120",
pages = "155--163",
journal = "Development",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Cytoplasmic calcium and Fucus egg activation

AU - Roberts, Stephen

AU - Gillot, I.

AU - Brownlee, Colin

PY - 1994/1

Y1 - 1994/1

N2 - Eggs of the marine brown alga, Fucus serratus, exhibit small transient elevations of cytosolic Ca2+ of variable magnitude, corresponding to the onset of the fertilization potential. Microinjection of Ca2+ buffers (BAPTA (1-2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid)) at concentrations sufficient to block any global fertilization-associated Ca-cyt(2+) elevation did not inhibit egg activation (monitored as exocytosis of cell wall) or subsequent development. However, egg activation could be inhibited with higher buffer concentrations. Br(2)BAPTA (K-d = 1.6 mu M) was a more effective inhibitor of egg activation than BAPTA (K-d = 0.17 mu M). Localized microinjection of Ca2+ produced only localized cell wall exocytosis at the injection site. Eggs injected with Br(2)BAPTA at intracellular concentrations, which blocked egg activation, exhibited prolonged fertilization potentials. Ca-45(2+) influx across the plasma membrane increased during fertilization. Our results show that a large transient global elevation of Ca-cyt(2+) is not necessary for Fucus egg activation but rather a localized elevation to micromolar levels results, at least in part, from increased Ca2+ influx across the plasma membrane. This is needed for early fertilization events, including the generation of the fertilization potential and cell wall secretion.

AB - Eggs of the marine brown alga, Fucus serratus, exhibit small transient elevations of cytosolic Ca2+ of variable magnitude, corresponding to the onset of the fertilization potential. Microinjection of Ca2+ buffers (BAPTA (1-2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid)) at concentrations sufficient to block any global fertilization-associated Ca-cyt(2+) elevation did not inhibit egg activation (monitored as exocytosis of cell wall) or subsequent development. However, egg activation could be inhibited with higher buffer concentrations. Br(2)BAPTA (K-d = 1.6 mu M) was a more effective inhibitor of egg activation than BAPTA (K-d = 0.17 mu M). Localized microinjection of Ca2+ produced only localized cell wall exocytosis at the injection site. Eggs injected with Br(2)BAPTA at intracellular concentrations, which blocked egg activation, exhibited prolonged fertilization potentials. Ca-45(2+) influx across the plasma membrane increased during fertilization. Our results show that a large transient global elevation of Ca-cyt(2+) is not necessary for Fucus egg activation but rather a localized elevation to micromolar levels results, at least in part, from increased Ca2+ influx across the plasma membrane. This is needed for early fertilization events, including the generation of the fertilization potential and cell wall secretion.

KW - FUCUS

KW - EGG ACTIVATION

KW - CYTOPLASMIC CALCIUM

KW - FURA-2-DEXTRAN

KW - BAPTA

KW - SEA-URCHIN EGGS

KW - URECHIS-CAUPO EGGS

KW - CORTICAL REACTION

KW - FERTILIZATION

KW - SPERM

KW - INCREASE

KW - INFLUX

KW - WAVE

KW - CHANNELS

KW - EVENTS

M3 - Journal article

VL - 120

SP - 155

EP - 163

JO - Development

JF - Development

SN - 0950-1991

IS - 1

ER -