Rights statement: This is the peer reviewed version of the following article: Navaei, T., Milan, P.B., Samadikuchaksaraei, A., Davari, H.R., Hardy, J.G. and Mozafari, M. (2021), Design and fabrication of polycaprolactone/gelatin composite scaffolds for diaphragmatic muscle reconstruction. J Tissue Eng Regen Med, 15: 78-87. doi: 10.1002/term.3151 which has been published in final form at https://onlinelibrary.wiley.com/doi/abs/10.1002/term.3151 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
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Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Design and fabrication of polycaprolactone/gelatin composite scaffolds for diaphragmatic muscle reconstruction
AU - Navaei, Tina
AU - Milan, Peiman
AU - Samadikuchaksaraei, Ali
AU - Davari, Hamid
AU - Hardy, John
AU - Mozafari, Masoud
N1 - This is the peer reviewed version of the following article: Navaei, T., Milan, P.B., Samadikuchaksaraei, A., Davari, H.R., Hardy, J.G. and Mozafari, M. (2021), Design and fabrication of polycaprolactone/gelatin composite scaffolds for diaphragmatic muscle reconstruction. J Tissue Eng Regen Med, 15: 78-87. doi: 10.1002/term.3151 which has been published in final form at https://onlinelibrary.wiley.com/doi/abs/10.1002/term.3151 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
PY - 2021/1/1
Y1 - 2021/1/1
N2 - Diaphragmatic wall defects caused by congenital disorders or disease remain a major challenge for physicians worldwide. Polymeric patches have been extensively explored within research laboratories and the clinic for soft tissue and diaphragm reconstruction. However, patch usage may be associated with allergic reaction, infection, granulation, and recurrence of the hernia. In this study, we designed and fabricated a porous scaffold using a combination of 3D printing and freeze-drying techniques. A 3D printed polycaprolactone (PCL) mesh was used to reinforcegelatin scaffolds, representing an advantage over previously reported examples since it provides mechanical strength and flexibility. In vitro studies showed that adherent cells were anchorage-dependent and grew as a monolayer attached to the scaffolds. Microscopic observations indicated better cell attachments for the scaffolds with higher gelatin content as compared with the PCL control samples. Tensile testing demonstrated the mechanical strength of samples was significantly greater than adult diaphragm tissue. The biocompatibility of the specimens was investigated in vivo using a subcutaneous implantation method in BALB/c adult mice for 20 days, with the results indicating superior cellular behavior and attachment on scaffolds containing gelatin in comparison to pure PCL scaffolds, suggesting that the porous PCL/gelatin scaffolds have potential as biodegradable and flexible constructs for diaphragm reconstruction.
AB - Diaphragmatic wall defects caused by congenital disorders or disease remain a major challenge for physicians worldwide. Polymeric patches have been extensively explored within research laboratories and the clinic for soft tissue and diaphragm reconstruction. However, patch usage may be associated with allergic reaction, infection, granulation, and recurrence of the hernia. In this study, we designed and fabricated a porous scaffold using a combination of 3D printing and freeze-drying techniques. A 3D printed polycaprolactone (PCL) mesh was used to reinforcegelatin scaffolds, representing an advantage over previously reported examples since it provides mechanical strength and flexibility. In vitro studies showed that adherent cells were anchorage-dependent and grew as a monolayer attached to the scaffolds. Microscopic observations indicated better cell attachments for the scaffolds with higher gelatin content as compared with the PCL control samples. Tensile testing demonstrated the mechanical strength of samples was significantly greater than adult diaphragm tissue. The biocompatibility of the specimens was investigated in vivo using a subcutaneous implantation method in BALB/c adult mice for 20 days, with the results indicating superior cellular behavior and attachment on scaffolds containing gelatin in comparison to pure PCL scaffolds, suggesting that the porous PCL/gelatin scaffolds have potential as biodegradable and flexible constructs for diaphragm reconstruction.
KW - biomaterials
KW - cellular response
KW - diaphragm
KW - regeneration
KW - scaffold
KW - tissue engineering
U2 - 10.1002/term.3151
DO - 10.1002/term.3151
M3 - Journal article
VL - 15
SP - 78
EP - 87
JO - Journal of Tissue Engineering and Regenerative Medicine
JF - Journal of Tissue Engineering and Regenerative Medicine
SN - 1932-6254
IS - 1
ER -