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Detailed analysis of an experimental challenge model for Leishmania infantum (JPC strain) in dogs

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Detailed analysis of an experimental challenge model for Leishmania infantum (JPC strain) in dogs. / Poot, Jacqueline; Rogers, Matthew E; Bates, Paul A et al.
In: Veterinary Parasitology, Vol. 130, No. 1-2, 06.2005, p. 41-53.

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Poot J, Rogers ME, Bates PA, Vermeulen A. Detailed analysis of an experimental challenge model for Leishmania infantum (JPC strain) in dogs. Veterinary Parasitology. 2005 Jun;130(1-2):41-53. doi: 10.1016/j.vetpar.2005.03.002

Author

Poot, Jacqueline ; Rogers, Matthew E ; Bates, Paul A et al. / Detailed analysis of an experimental challenge model for Leishmania infantum (JPC strain) in dogs. In: Veterinary Parasitology. 2005 ; Vol. 130, No. 1-2. pp. 41-53.

Bibtex

@article{3f4b0be00afe47ea93cb89901b34864a,
title = "Detailed analysis of an experimental challenge model for Leishmania infantum (JPC strain) in dogs",
abstract = "In this study, disease progression after intravenous or subdermal infection of dogs with Leishmania infantum JPC strain was monitored. A challenge performed on 14 dogs via the intravenous route with 5 x 10(7) stationary phase promastigotes of the L. infantum JPC strain was 100% successful. During a follow up period of 1.5 years, several parameters were evaluated in order to find the most reliable disease markers. Parasite detection by culture and histology were found to be very sensitive (100%). Additionally, regular physical examination, serology and serum gamma-globulin levels were found to be useful parameters in the evaluation of disease severity and are recommended for inclusion in vaccination-challenge experiments. Although this intravenous challenge model has practical limitations, the data set confirms it is the best experimental model currently available for vaccine development. Two intravenously infected dogs were treated with corticosteroids for 5 months. This treatment was shown to enhance all aspects of a Leishmania infection. Five more dogs were infected by sub-dermal injection of promastigotes mixed with a proteophosphoglycan-matrix (PSG) secreted by Leishmania that assists in transmission and infection by sand fly bite. The resulting parasite burdens were low and the animals remained asymptomatic during a 2-year follow up period. However, this procedure did result in infection in 80% of the dogs and is appealing for future development as a natural challenge model in vaccine development.",
keywords = "Leishmania infantum, Dog , Experimental infection , Immune response , PSG",
author = "Jacqueline Poot and Rogers, {Matthew E} and Bates, {Paul A} and Arno Vermeulen",
year = "2005",
month = jun,
doi = "10.1016/j.vetpar.2005.03.002",
language = "English",
volume = "130",
pages = "41--53",
journal = "Veterinary Parasitology",
issn = "0304-4017",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Detailed analysis of an experimental challenge model for Leishmania infantum (JPC strain) in dogs

AU - Poot, Jacqueline

AU - Rogers, Matthew E

AU - Bates, Paul A

AU - Vermeulen, Arno

PY - 2005/6

Y1 - 2005/6

N2 - In this study, disease progression after intravenous or subdermal infection of dogs with Leishmania infantum JPC strain was monitored. A challenge performed on 14 dogs via the intravenous route with 5 x 10(7) stationary phase promastigotes of the L. infantum JPC strain was 100% successful. During a follow up period of 1.5 years, several parameters were evaluated in order to find the most reliable disease markers. Parasite detection by culture and histology were found to be very sensitive (100%). Additionally, regular physical examination, serology and serum gamma-globulin levels were found to be useful parameters in the evaluation of disease severity and are recommended for inclusion in vaccination-challenge experiments. Although this intravenous challenge model has practical limitations, the data set confirms it is the best experimental model currently available for vaccine development. Two intravenously infected dogs were treated with corticosteroids for 5 months. This treatment was shown to enhance all aspects of a Leishmania infection. Five more dogs were infected by sub-dermal injection of promastigotes mixed with a proteophosphoglycan-matrix (PSG) secreted by Leishmania that assists in transmission and infection by sand fly bite. The resulting parasite burdens were low and the animals remained asymptomatic during a 2-year follow up period. However, this procedure did result in infection in 80% of the dogs and is appealing for future development as a natural challenge model in vaccine development.

AB - In this study, disease progression after intravenous or subdermal infection of dogs with Leishmania infantum JPC strain was monitored. A challenge performed on 14 dogs via the intravenous route with 5 x 10(7) stationary phase promastigotes of the L. infantum JPC strain was 100% successful. During a follow up period of 1.5 years, several parameters were evaluated in order to find the most reliable disease markers. Parasite detection by culture and histology were found to be very sensitive (100%). Additionally, regular physical examination, serology and serum gamma-globulin levels were found to be useful parameters in the evaluation of disease severity and are recommended for inclusion in vaccination-challenge experiments. Although this intravenous challenge model has practical limitations, the data set confirms it is the best experimental model currently available for vaccine development. Two intravenously infected dogs were treated with corticosteroids for 5 months. This treatment was shown to enhance all aspects of a Leishmania infection. Five more dogs were infected by sub-dermal injection of promastigotes mixed with a proteophosphoglycan-matrix (PSG) secreted by Leishmania that assists in transmission and infection by sand fly bite. The resulting parasite burdens were low and the animals remained asymptomatic during a 2-year follow up period. However, this procedure did result in infection in 80% of the dogs and is appealing for future development as a natural challenge model in vaccine development.

KW - Leishmania infantum

KW - Dog

KW - Experimental infection

KW - Immune response

KW - PSG

U2 - 10.1016/j.vetpar.2005.03.002

DO - 10.1016/j.vetpar.2005.03.002

M3 - Journal article

C2 - 15893068

VL - 130

SP - 41

EP - 53

JO - Veterinary Parasitology

JF - Veterinary Parasitology

SN - 0304-4017

IS - 1-2

ER -