Rights statement: This is the peer reviewed version of the following article: Polanco F., A., Mutis Martinezguerra, M., Marques, V., Villa-Navarro, F., Borrero Pérez, G. H., Cheutin, M.-C., Dejean, T., Hocdé, R., Juhel, J.-B., Maire, E., Manel, S., Spescha, M., Valentini, A., Mouillot, D., Albouy, C., & Pellissier, L. (2021). Detecting aquatic and terrestrial biodiversity in a tropical estuary using environmental DNA. Biotropica, 00, 1– 14. https://doi.org/10.1111/btp.13009 which has been published in final form at https://onlinelibrary.wiley.com/doi/10.1111/btp.13009 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
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Final published version
Licence: CC BY-NC: Creative Commons Attribution-NonCommercial 4.0 International License
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Detecting aquatic and terrestrial biodiversity in a tropical estuary using environmental DNA
AU - Polanco F., A.
AU - Mutis Martinezguerra, M.
AU - Marques, V.
AU - Villa-Navarro, F.
AU - Borrero Pérez, G.H.
AU - Cheutin, M.-C.
AU - Dejean, T.
AU - Hocdé, R.
AU - Juhel, J.-B.
AU - Maire, E.
AU - Manel, S.
AU - Spescha, M.
AU - Valentini, A.
AU - Mouillot, D.
AU - Albouy, C.
AU - Pellissier, L.
N1 - This is the peer reviewed version of the following article: Polanco F., A., Mutis Martinezguerra, M., Marques, V., Villa-Navarro, F., Borrero Pérez, G. H., Cheutin, M.-C., Dejean, T., Hocdé, R., Juhel, J.-B., Maire, E., Manel, S., Spescha, M., Valentini, A., Mouillot, D., Albouy, C., & Pellissier, L. (2021). Detecting aquatic and terrestrial biodiversity in a tropical estuary using environmental DNA. Biotropica, 00, 1– 14. https://doi.org/10.1111/btp.13009 which has been published in final form at https://onlinelibrary.wiley.com/doi/10.1111/btp.13009 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
PY - 2021/8/12
Y1 - 2021/8/12
N2 - Estuaries are characterized by a tidal regime and are strongly influenced by hydrodynamics and host diverse and highly dynamic habitats, from fresh, brackish, or saltwater to terrestrial, whose biodiversity is especially difficult to monitor. Here, we investigated the potential of environmental DNA (eDNA) metabarcoding, with three primer sets targeting different regions of the mitochondrial DNA 12S ribosomal RNA gene, to detect vertebrate diversity in the estuary of the Don Diego River in Colombia. With eDNA, we detected not only aquatic organisms, including fishes, amphibians, and reptiles, but also a large diversity of terrestrial, arboreal, and flying vertebrates, including mammals and birds, living in the estuary surroundings. Further, the eDNA signal remained relatively localized along the watercourse. A transect from the deep outer section of the estuary, across the river mouth toward the inner section of the river, showed marked taxonomic turnover from typical marine to freshwater fishes, while eDNA of terrestrial and arboreal species was mainly found in the inner section of the estuary. Our results indicate that eDNA enables the detection of a large diversity of vertebrates and could become an important tool for biodiversity monitoring in estuaries, where water integrates information across the ecosystem. Abstract in Spanish is available with online material.
AB - Estuaries are characterized by a tidal regime and are strongly influenced by hydrodynamics and host diverse and highly dynamic habitats, from fresh, brackish, or saltwater to terrestrial, whose biodiversity is especially difficult to monitor. Here, we investigated the potential of environmental DNA (eDNA) metabarcoding, with three primer sets targeting different regions of the mitochondrial DNA 12S ribosomal RNA gene, to detect vertebrate diversity in the estuary of the Don Diego River in Colombia. With eDNA, we detected not only aquatic organisms, including fishes, amphibians, and reptiles, but also a large diversity of terrestrial, arboreal, and flying vertebrates, including mammals and birds, living in the estuary surroundings. Further, the eDNA signal remained relatively localized along the watercourse. A transect from the deep outer section of the estuary, across the river mouth toward the inner section of the river, showed marked taxonomic turnover from typical marine to freshwater fishes, while eDNA of terrestrial and arboreal species was mainly found in the inner section of the estuary. Our results indicate that eDNA enables the detection of a large diversity of vertebrates and could become an important tool for biodiversity monitoring in estuaries, where water integrates information across the ecosystem. Abstract in Spanish is available with online material.
KW - biodiversity
KW - biomonitoring
KW - Caribbean Sea
KW - Colombia
KW - Don Diego River
KW - environmental DNA
KW - Sierra Nevada de Santa Marta
KW - tropical ecosystem
KW - vertebrate
U2 - 10.1111/btp.13009
DO - 10.1111/btp.13009
M3 - Journal article
JO - Biotropica
JF - Biotropica
SN - 0006-3606
ER -