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    Rights statement: http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 118 (1), pp 27-32 1999, © 1999 Cambridge University Press.

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Detection of Leishmania lipophosphoglycan binding proteins in the gut of the sandfly vector

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Detection of Leishmania lipophosphoglycan binding proteins in the gut of the sandfly vector. / Dillon, R. J. ; Lane, R. P. .
In: Parasitology, Vol. 118, No. 1, 01.1999, p. 27-32.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Dillon, RJ & Lane, RP 1999, 'Detection of Leishmania lipophosphoglycan binding proteins in the gut of the sandfly vector', Parasitology, vol. 118, no. 1, pp. 27-32. <http://journals.cambridge.org/action/displayJournal?jid=PAR>

APA

Dillon, R. J., & Lane, R. P. (1999). Detection of Leishmania lipophosphoglycan binding proteins in the gut of the sandfly vector. Parasitology, 118(1), 27-32. http://journals.cambridge.org/action/displayJournal?jid=PAR

Vancouver

Dillon RJ, Lane RP. Detection of Leishmania lipophosphoglycan binding proteins in the gut of the sandfly vector. Parasitology. 1999 Jan;118(1):27-32.

Author

Dillon, R. J. ; Lane, R. P. . / Detection of Leishmania lipophosphoglycan binding proteins in the gut of the sandfly vector. In: Parasitology. 1999 ; Vol. 118, No. 1. pp. 27-32.

Bibtex

@article{6c054a3ef9644285b8a8cddf276a79ec,
title = "Detection of Leishmania lipophosphoglycan binding proteins in the gut of the sandfly vector",
abstract = "Binding to the midgut microvillar surface in the sandfly Phlebotomus papatasi is a prerequisite for successful development of Leishmania major within the gut of the vector. This paper describes a method for detecting microvillar-associated proteins which act as ligands for the parasite surface glycoconjugate lipophosphoglycan (LPG). Adhesion of LPG to midgut proteins was visualized by probing midgut extracts with LPG using a Western ligand blotting technique. Procyclic L. major LPG bound to a microvillar polypeptide band of 65 kDa (estimated in the non-reduced state) and bound variably to several lower molecular weight bands, probably degradation products or subunits of the primary binding polypeptides. Specificity of binding was confirmed by co-incubating biotinylated LPG with an LPG-specific mAb which resulted in a great reduction in binding.",
keywords = "Leishmania major, lipophosphoglycan , Phlebotomus papatasi , microvilli , midgut",
author = "Dillon, {R. J.} and Lane, {R. P.}",
note = "http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 118 (1), pp 27-32 1999, {\textcopyright} 1999 Cambridge University Press.",
year = "1999",
month = jan,
language = "English",
volume = "118",
pages = "27--32",
journal = "Parasitology",
issn = "0031-1820",
publisher = "Cambridge University Press",
number = "1",

}

RIS

TY - JOUR

T1 - Detection of Leishmania lipophosphoglycan binding proteins in the gut of the sandfly vector

AU - Dillon, R. J.

AU - Lane, R. P.

N1 - http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 118 (1), pp 27-32 1999, © 1999 Cambridge University Press.

PY - 1999/1

Y1 - 1999/1

N2 - Binding to the midgut microvillar surface in the sandfly Phlebotomus papatasi is a prerequisite for successful development of Leishmania major within the gut of the vector. This paper describes a method for detecting microvillar-associated proteins which act as ligands for the parasite surface glycoconjugate lipophosphoglycan (LPG). Adhesion of LPG to midgut proteins was visualized by probing midgut extracts with LPG using a Western ligand blotting technique. Procyclic L. major LPG bound to a microvillar polypeptide band of 65 kDa (estimated in the non-reduced state) and bound variably to several lower molecular weight bands, probably degradation products or subunits of the primary binding polypeptides. Specificity of binding was confirmed by co-incubating biotinylated LPG with an LPG-specific mAb which resulted in a great reduction in binding.

AB - Binding to the midgut microvillar surface in the sandfly Phlebotomus papatasi is a prerequisite for successful development of Leishmania major within the gut of the vector. This paper describes a method for detecting microvillar-associated proteins which act as ligands for the parasite surface glycoconjugate lipophosphoglycan (LPG). Adhesion of LPG to midgut proteins was visualized by probing midgut extracts with LPG using a Western ligand blotting technique. Procyclic L. major LPG bound to a microvillar polypeptide band of 65 kDa (estimated in the non-reduced state) and bound variably to several lower molecular weight bands, probably degradation products or subunits of the primary binding polypeptides. Specificity of binding was confirmed by co-incubating biotinylated LPG with an LPG-specific mAb which resulted in a great reduction in binding.

KW - Leishmania major

KW - lipophosphoglycan

KW - Phlebotomus papatasi

KW - microvilli

KW - midgut

M3 - Journal article

VL - 118

SP - 27

EP - 32

JO - Parasitology

JF - Parasitology

SN - 0031-1820

IS - 1

ER -