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Detection of Mycobacterium immunogenum by real-time quantitative Taqman PCR

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Detection of Mycobacterium immunogenum by real-time quantitative Taqman PCR. / Rhodes, Glenn; Fluri, Alexandra; Gerber, Marco et al.
In: Journal of Microbiological Methods, Vol. 73, No. 3, 30.06.2008, p. 266-268.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Rhodes, G, Fluri, A, Gerber, M, Henderson, A, Ruefenacht, A & Pickup, RW 2008, 'Detection of Mycobacterium immunogenum by real-time quantitative Taqman PCR', Journal of Microbiological Methods, vol. 73, no. 3, pp. 266-268. https://doi.org/10.1016/j.mimet.2008.03.006

APA

Rhodes, G., Fluri, A., Gerber, M., Henderson, A., Ruefenacht, A., & Pickup, R. W. (2008). Detection of Mycobacterium immunogenum by real-time quantitative Taqman PCR. Journal of Microbiological Methods, 73(3), 266-268. https://doi.org/10.1016/j.mimet.2008.03.006

Vancouver

Rhodes G, Fluri A, Gerber M, Henderson A, Ruefenacht A, Pickup RW. Detection of Mycobacterium immunogenum by real-time quantitative Taqman PCR. Journal of Microbiological Methods. 2008 Jun 30;73(3):266-268. doi: 10.1016/j.mimet.2008.03.006

Author

Rhodes, Glenn ; Fluri, Alexandra ; Gerber, Marco et al. / Detection of Mycobacterium immunogenum by real-time quantitative Taqman PCR. In: Journal of Microbiological Methods. 2008 ; Vol. 73, No. 3. pp. 266-268.

Bibtex

@article{18bb5298b250439cba6f6ee2174f1f87,
title = "Detection of Mycobacterium immunogenum by real-time quantitative Taqman PCR",
abstract = "A quantitative real-time 5′-nuclease (Taqman) PCR technique was developed to specifically detect Mycobacterium immunogenum. rpoB-specific primers and Taqman probe were evaluated for detection of M. immunogenum DNA extracted from pure cultures and from industrial metal working fluids (MWFs). Specificity was confirmed and the sensitivity of detection of M. immunogenum genomic DNA was shown to be approximately 9 fg (2 cell equivalents). When tested on industrial metal working fluids from the UK and USA from which no M. immunogenum CFU were recovered, the assay detected between 3.4 × 101 and 1.9 × 104 cell equivalents (CE) per ml, and increased the detection rate over culture to 37.5% (12 of 32 samples). This assay provides a specific, sensitive and rapid method for the detection of M. immunogenum and is applicable within industry for the early detection of this human pathogen and to the possible prevention of hypersensitivity pneumonitis (HP) in workers.",
keywords = "Hypersensitivity pneumonitis, Metal working fluids, Mycobacterium chelonae complex, Mycobacterium immunogenum, Real-time PCR, Taqman",
author = "Glenn Rhodes and Alexandra Fluri and Marco Gerber and Alan Henderson and Andrea Ruefenacht and Pickup, {Roger W.}",
year = "2008",
month = jun,
day = "30",
doi = "10.1016/j.mimet.2008.03.006",
language = "English",
volume = "73",
pages = "266--268",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Detection of Mycobacterium immunogenum by real-time quantitative Taqman PCR

AU - Rhodes, Glenn

AU - Fluri, Alexandra

AU - Gerber, Marco

AU - Henderson, Alan

AU - Ruefenacht, Andrea

AU - Pickup, Roger W.

PY - 2008/6/30

Y1 - 2008/6/30

N2 - A quantitative real-time 5′-nuclease (Taqman) PCR technique was developed to specifically detect Mycobacterium immunogenum. rpoB-specific primers and Taqman probe were evaluated for detection of M. immunogenum DNA extracted from pure cultures and from industrial metal working fluids (MWFs). Specificity was confirmed and the sensitivity of detection of M. immunogenum genomic DNA was shown to be approximately 9 fg (2 cell equivalents). When tested on industrial metal working fluids from the UK and USA from which no M. immunogenum CFU were recovered, the assay detected between 3.4 × 101 and 1.9 × 104 cell equivalents (CE) per ml, and increased the detection rate over culture to 37.5% (12 of 32 samples). This assay provides a specific, sensitive and rapid method for the detection of M. immunogenum and is applicable within industry for the early detection of this human pathogen and to the possible prevention of hypersensitivity pneumonitis (HP) in workers.

AB - A quantitative real-time 5′-nuclease (Taqman) PCR technique was developed to specifically detect Mycobacterium immunogenum. rpoB-specific primers and Taqman probe were evaluated for detection of M. immunogenum DNA extracted from pure cultures and from industrial metal working fluids (MWFs). Specificity was confirmed and the sensitivity of detection of M. immunogenum genomic DNA was shown to be approximately 9 fg (2 cell equivalents). When tested on industrial metal working fluids from the UK and USA from which no M. immunogenum CFU were recovered, the assay detected between 3.4 × 101 and 1.9 × 104 cell equivalents (CE) per ml, and increased the detection rate over culture to 37.5% (12 of 32 samples). This assay provides a specific, sensitive and rapid method for the detection of M. immunogenum and is applicable within industry for the early detection of this human pathogen and to the possible prevention of hypersensitivity pneumonitis (HP) in workers.

KW - Hypersensitivity pneumonitis

KW - Metal working fluids

KW - Mycobacterium chelonae complex

KW - Mycobacterium immunogenum

KW - Real-time PCR

KW - Taqman

U2 - 10.1016/j.mimet.2008.03.006

DO - 10.1016/j.mimet.2008.03.006

M3 - Journal article

C2 - 18423662

AN - SCOPUS:43049157955

VL - 73

SP - 266

EP - 268

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

IS - 3

ER -