Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Determination of carcinoembryonic antigen (CEA) by surface plasmon resonance-enhanced total internal reflection ellipsometry (SPRe-TIRE)
AU - Erkal-Aytemur, Aslı
AU - Şahin, Samet
AU - Üstündağ, Zafer
AU - Mülazımoğlu, İbrahim Ender
AU - Caglayan, Mustafa Oguzhan
PY - 2023/8/7
Y1 - 2023/8/7
N2 - A surface plasmon resonance-enhanced total internal reflection ellipsometric (SPRe-TIRE) aptasensor is reported for the determination of the carcinoembryonic antigen (CEA). A gold nanofilm-coated SF10 glass slide was modified with electrochemical diazonium salt reduction and gold nanoparticles (AuNPs) to form a sensor. Next, a specific aptamer for CEA was immobilized on the AuNPs via self-assembly. The platform was characterized by X-ray photoelectron spectroscopy, ellipsometry, and cyclic voltammetry for each step of the preparation of the sensor. CEA aptamers with different affinities were used. SPRe-TIRE employed total internal reflection geometry and AuNPs to enhance plasmon resonance for the sensitive and selective determination of CEA in human serum. The working range of the aptasensor was from 0.01 to 500 ng/mL with an optimum detection limit of 0.1 pg/mL.
AB - A surface plasmon resonance-enhanced total internal reflection ellipsometric (SPRe-TIRE) aptasensor is reported for the determination of the carcinoembryonic antigen (CEA). A gold nanofilm-coated SF10 glass slide was modified with electrochemical diazonium salt reduction and gold nanoparticles (AuNPs) to form a sensor. Next, a specific aptamer for CEA was immobilized on the AuNPs via self-assembly. The platform was characterized by X-ray photoelectron spectroscopy, ellipsometry, and cyclic voltammetry for each step of the preparation of the sensor. CEA aptamers with different affinities were used. SPRe-TIRE employed total internal reflection geometry and AuNPs to enhance plasmon resonance for the sensitive and selective determination of CEA in human serum. The working range of the aptasensor was from 0.01 to 500 ng/mL with an optimum detection limit of 0.1 pg/mL.
KW - aptasensor
KW - Carcinoembryonic antigen (CEA)
KW - cyclic voltammetry (CV)
KW - surface plasmon resonance-enhanced total internal reflection ellipsometry (SPRe-TIRE)
U2 - 10.1080/10739149.2023.2242921
DO - 10.1080/10739149.2023.2242921
M3 - Journal article
AN - SCOPUS:85166968824
JO - Instrumentation Science and Technology
JF - Instrumentation Science and Technology
SN - 1073-9149
ER -