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Determination of water content in dehydrated mammalian cells using terahertz pulsed imaging: a feasibility study

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Determination of water content in dehydrated mammalian cells using terahertz pulsed imaging : a feasibility study. / Chau, David Y. S.; Dennis, Anthony R.; Lin, Hungyen; Zeitler, J. Axel; Tunnacliffe, Alan.

In: Current Pharmaceutical Biotechnology, Vol. 17, No. 2, 2016, p. 200-207.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Chau, DYS, Dennis, AR, Lin, H, Zeitler, JA & Tunnacliffe, A 2016, 'Determination of water content in dehydrated mammalian cells using terahertz pulsed imaging: a feasibility study', Current Pharmaceutical Biotechnology, vol. 17, no. 2, pp. 200-207. https://doi.org/10.2174/1389201017666151029105941

APA

Chau, D. Y. S., Dennis, A. R., Lin, H., Zeitler, J. A., & Tunnacliffe, A. (2016). Determination of water content in dehydrated mammalian cells using terahertz pulsed imaging: a feasibility study. Current Pharmaceutical Biotechnology, 17(2), 200-207. https://doi.org/10.2174/1389201017666151029105941

Vancouver

Author

Chau, David Y. S. ; Dennis, Anthony R. ; Lin, Hungyen ; Zeitler, J. Axel ; Tunnacliffe, Alan. / Determination of water content in dehydrated mammalian cells using terahertz pulsed imaging : a feasibility study. In: Current Pharmaceutical Biotechnology. 2016 ; Vol. 17, No. 2. pp. 200-207.

Bibtex

@article{3697fd8963b14f7cac8d75bfe4dc9aeb,
title = "Determination of water content in dehydrated mammalian cells using terahertz pulsed imaging: a feasibility study",
abstract = "Mammalian cells are involved in a range of biotechnological applications and more recently have been increasingly exploited in regenerative medicine. Critical to successful applications involving mammalian cells are their long-term storage and transport, for which cryopreservation in liquid nitrogen is the most frequently used strategy. However, cryopreservation suffers from high costs, difficulties in transport logistics and the use of undesirable additives (e.g. animal sera or DMSO). An alternative approach, proposed as low cost, low maintenance and process-compatible, is viable desiccation of mammalian cells. Several groups claim to have achieved this, but the extent of desiccation in the cell samples concerned is not always clear, in part because of difficulties in determining very low water content. Although several techniques exist that are frequently used to quantify the amount of water in samples (e.g. FTIR spectroscopy, thermogravimetric analysis (TGA), NMR spectroscopy), the complexity of sample preparation, as well as the costs and time constraints involved are disadvantageous. Here, we assess a novel, rapid and low cost technique, i.e. terahertz (THz) spectroscopy, for the quantification of water content within dehydrated mammalian cell samples.",
keywords = "Dehydration, desiccation, terahertz pulsed imaging, humidity, anhydrobiotic engineering",
author = "Chau, {David Y. S.} and Dennis, {Anthony R.} and Hungyen Lin and Zeitler, {J. Axel} and Alan Tunnacliffe",
year = "2016",
doi = "10.2174/1389201017666151029105941",
language = "English",
volume = "17",
pages = "200--207",
journal = "Current Pharmaceutical Biotechnology",
issn = "1389-2010",
publisher = "Bentham Science Publishers B.V.",
number = "2",

}

RIS

TY - JOUR

T1 - Determination of water content in dehydrated mammalian cells using terahertz pulsed imaging

T2 - a feasibility study

AU - Chau, David Y. S.

AU - Dennis, Anthony R.

AU - Lin, Hungyen

AU - Zeitler, J. Axel

AU - Tunnacliffe, Alan

PY - 2016

Y1 - 2016

N2 - Mammalian cells are involved in a range of biotechnological applications and more recently have been increasingly exploited in regenerative medicine. Critical to successful applications involving mammalian cells are their long-term storage and transport, for which cryopreservation in liquid nitrogen is the most frequently used strategy. However, cryopreservation suffers from high costs, difficulties in transport logistics and the use of undesirable additives (e.g. animal sera or DMSO). An alternative approach, proposed as low cost, low maintenance and process-compatible, is viable desiccation of mammalian cells. Several groups claim to have achieved this, but the extent of desiccation in the cell samples concerned is not always clear, in part because of difficulties in determining very low water content. Although several techniques exist that are frequently used to quantify the amount of water in samples (e.g. FTIR spectroscopy, thermogravimetric analysis (TGA), NMR spectroscopy), the complexity of sample preparation, as well as the costs and time constraints involved are disadvantageous. Here, we assess a novel, rapid and low cost technique, i.e. terahertz (THz) spectroscopy, for the quantification of water content within dehydrated mammalian cell samples.

AB - Mammalian cells are involved in a range of biotechnological applications and more recently have been increasingly exploited in regenerative medicine. Critical to successful applications involving mammalian cells are their long-term storage and transport, for which cryopreservation in liquid nitrogen is the most frequently used strategy. However, cryopreservation suffers from high costs, difficulties in transport logistics and the use of undesirable additives (e.g. animal sera or DMSO). An alternative approach, proposed as low cost, low maintenance and process-compatible, is viable desiccation of mammalian cells. Several groups claim to have achieved this, but the extent of desiccation in the cell samples concerned is not always clear, in part because of difficulties in determining very low water content. Although several techniques exist that are frequently used to quantify the amount of water in samples (e.g. FTIR spectroscopy, thermogravimetric analysis (TGA), NMR spectroscopy), the complexity of sample preparation, as well as the costs and time constraints involved are disadvantageous. Here, we assess a novel, rapid and low cost technique, i.e. terahertz (THz) spectroscopy, for the quantification of water content within dehydrated mammalian cell samples.

KW - Dehydration

KW - desiccation

KW - terahertz pulsed imaging

KW - humidity

KW - anhydrobiotic engineering

U2 - 10.2174/1389201017666151029105941

DO - 10.2174/1389201017666151029105941

M3 - Journal article

VL - 17

SP - 200

EP - 207

JO - Current Pharmaceutical Biotechnology

JF - Current Pharmaceutical Biotechnology

SN - 1389-2010

IS - 2

ER -