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Development and application of molecular tools in the study of IncN-related plasmids from lakewater sediments

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Development and application of molecular tools in the study of IncN-related plasmids from lakewater sediments. / Osborn, A. Mark; Pickup, Roger W.; Saunders, Jon R.
In: FEMS Microbiology Letters, Vol. 186, No. 2, 05.05.2000, p. 203-208.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Osborn, AM, Pickup, RW & Saunders, JR 2000, 'Development and application of molecular tools in the study of IncN-related plasmids from lakewater sediments', FEMS Microbiology Letters, vol. 186, no. 2, pp. 203-208. https://doi.org/10.1016/S0378-1097(00)00147-6

APA

Osborn, A. M., Pickup, R. W., & Saunders, J. R. (2000). Development and application of molecular tools in the study of IncN-related plasmids from lakewater sediments. FEMS Microbiology Letters, 186(2), 203-208. https://doi.org/10.1016/S0378-1097(00)00147-6

Vancouver

Osborn AM, Pickup RW, Saunders JR. Development and application of molecular tools in the study of IncN-related plasmids from lakewater sediments. FEMS Microbiology Letters. 2000 May 5;186(2):203-208. doi: 10.1016/S0378-1097(00)00147-6

Author

Osborn, A. Mark ; Pickup, Roger W. ; Saunders, Jon R. / Development and application of molecular tools in the study of IncN-related plasmids from lakewater sediments. In: FEMS Microbiology Letters. 2000 ; Vol. 186, No. 2. pp. 203-208.

Bibtex

@article{f408fe4379ff4d7b8608a9dee8b830ee,
title = "Development and application of molecular tools in the study of IncN-related plasmids from lakewater sediments",
abstract = "Homology to IncN, P, Q and W inc regions was investigated amongst 114 Hg2+-resistant or antibiotic-resistant bacteria isolated from lakewater sediments. No hybridisation signals were found with Inc P, Q and W probes, and only one plasmid, pLV1402, hybridised to the IncN probe. PCR primers designed to conserved regions in the replicon of the IncN plasmid pCU1 and the related beta replicon from pGSH500 were used to amplify a 978-bp fragment from pLV1402, with sequence analysis showing a close relationship (99.2% identity) between their replication genes. A 387-bp region from the pLV1402 rep gene was used to re-screen the isolates and identified another related plasmid, pLV1403. A 3.7-kb probe containing the alpha replicon from pGSH500 hybridised to both pLV1402 and pLV1403, suggesting that both are multi-replicon plasmids. The PCR primers and probes described will be useful in future studies of plasmid diversity. Copyright (C) 2000 Federation of European Microbiological Societies.",
keywords = "IncN, PCR, pGSH500, Plasmid, Replicon probing",
author = "Osborn, {A. Mark} and Pickup, {Roger W.} and Saunders, {Jon R.}",
year = "2000",
month = may,
day = "5",
doi = "10.1016/S0378-1097(00)00147-6",
language = "English",
volume = "186",
pages = "203--208",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Development and application of molecular tools in the study of IncN-related plasmids from lakewater sediments

AU - Osborn, A. Mark

AU - Pickup, Roger W.

AU - Saunders, Jon R.

PY - 2000/5/5

Y1 - 2000/5/5

N2 - Homology to IncN, P, Q and W inc regions was investigated amongst 114 Hg2+-resistant or antibiotic-resistant bacteria isolated from lakewater sediments. No hybridisation signals were found with Inc P, Q and W probes, and only one plasmid, pLV1402, hybridised to the IncN probe. PCR primers designed to conserved regions in the replicon of the IncN plasmid pCU1 and the related beta replicon from pGSH500 were used to amplify a 978-bp fragment from pLV1402, with sequence analysis showing a close relationship (99.2% identity) between their replication genes. A 387-bp region from the pLV1402 rep gene was used to re-screen the isolates and identified another related plasmid, pLV1403. A 3.7-kb probe containing the alpha replicon from pGSH500 hybridised to both pLV1402 and pLV1403, suggesting that both are multi-replicon plasmids. The PCR primers and probes described will be useful in future studies of plasmid diversity. Copyright (C) 2000 Federation of European Microbiological Societies.

AB - Homology to IncN, P, Q and W inc regions was investigated amongst 114 Hg2+-resistant or antibiotic-resistant bacteria isolated from lakewater sediments. No hybridisation signals were found with Inc P, Q and W probes, and only one plasmid, pLV1402, hybridised to the IncN probe. PCR primers designed to conserved regions in the replicon of the IncN plasmid pCU1 and the related beta replicon from pGSH500 were used to amplify a 978-bp fragment from pLV1402, with sequence analysis showing a close relationship (99.2% identity) between their replication genes. A 387-bp region from the pLV1402 rep gene was used to re-screen the isolates and identified another related plasmid, pLV1403. A 3.7-kb probe containing the alpha replicon from pGSH500 hybridised to both pLV1402 and pLV1403, suggesting that both are multi-replicon plasmids. The PCR primers and probes described will be useful in future studies of plasmid diversity. Copyright (C) 2000 Federation of European Microbiological Societies.

KW - IncN

KW - PCR

KW - pGSH500

KW - Plasmid

KW - Replicon probing

U2 - 10.1016/S0378-1097(00)00147-6

DO - 10.1016/S0378-1097(00)00147-6

M3 - Journal article

C2 - 10802172

AN - SCOPUS:0034607994

VL - 186

SP - 203

EP - 208

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -