Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Development of a dewaxing protocol for tissue-engineered models of the oral mucosa used for Raman spectroscopic analysis
AU - Mian, S.A.
AU - Colley, H.E.
AU - Thornhill, M.H.
AU - Rehman, I.U.
PY - 2014
Y1 - 2014
N2 - We demonstrate that wax-embedded models of tissue-engineered oral mucosa can be effectively dewaxed using xylene for Raman spectroscopic analysis. Tissue sections of 20 m thickness were cut and mounted onto glass slides. Sections were placed in xylene for increasing lengths from 2 to 45 min. Acquired Raman spectra revealed the wax contribution in the fingerprint region until 28-min treatment in xylene. Good quality and wax-free spectra were recorded at 30, 35, 40, and 45 min in xylene with no significant differences among them. It is essential to collect uncontaminated Raman spectra in order to achieve authentic results because the fingerprint region of biological tissues holds extremely vital information that is of diagnostic significance in cancer. © 2014 Taylor & Francis Group, LLC.
AB - We demonstrate that wax-embedded models of tissue-engineered oral mucosa can be effectively dewaxed using xylene for Raman spectroscopic analysis. Tissue sections of 20 m thickness were cut and mounted onto glass slides. Sections were placed in xylene for increasing lengths from 2 to 45 min. Acquired Raman spectra revealed the wax contribution in the fingerprint region until 28-min treatment in xylene. Good quality and wax-free spectra were recorded at 30, 35, 40, and 45 min in xylene with no significant differences among them. It is essential to collect uncontaminated Raman spectra in order to achieve authentic results because the fingerprint region of biological tissues holds extremely vital information that is of diagnostic significance in cancer. © 2014 Taylor & Francis Group, LLC.
KW - Analytical
KW - Biological
KW - Dewaxing protocol
KW - Oral mucosa
KW - Raman spectroscopy
KW - Sample preparations
KW - Dewaxing
KW - Raman scattering
KW - Xylene
KW - Biological tissues
KW - Glass slides
KW - Sample preparation
KW - Tissue sections
KW - Tissue
U2 - 10.1080/05704928.2014.882348
DO - 10.1080/05704928.2014.882348
M3 - Journal article
VL - 49
SP - 614
EP - 617
JO - APPLIED SPECTROSCOPY REVIEWS
JF - APPLIED SPECTROSCOPY REVIEWS
SN - 0570-4928
IS - 8
ER -