Development of a dewaxing protocol for tissue-engineered models of the oral mucosa used for Raman spectroscopic analysis

School of Engineering

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https://doi.org/10.1080/05704928.2014.882348
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Keywords

Analytical, Biological, Dewaxing protocol, Oral mucosa, Raman spectroscopy, Sample preparations, Dewaxing, Raman scattering, Xylene, Biological tissues, Glass slides, Sample preparation, Tissue sections, Tissue

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Development of a dewaxing protocol for tissue-engineered models of the oral mucosa used for Raman spectroscopic analysis. / Mian, S.A.; Colley, H.E.; Thornhill, M.H. et al.
In: APPLIED SPECTROSCOPY REVIEWS, Vol. 49, No. 8, 2014, p. 614-617.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Author

Mian, S.A. ; Colley, H.E. ; Thornhill, M.H. et al. / Development of a dewaxing protocol for tissue-engineered models of the oral mucosa used for Raman spectroscopic analysis. In: APPLIED SPECTROSCOPY REVIEWS. 2014 ; Vol. 49, No. 8. pp. 614-617.

Bibtex

@article{f6738e412b124720a4d5d41484fc86c0,

title = "Development of a dewaxing protocol for tissue-engineered models of the oral mucosa used for Raman spectroscopic analysis",

abstract = "We demonstrate that wax-embedded models of tissue-engineered oral mucosa can be effectively dewaxed using xylene for Raman spectroscopic analysis. Tissue sections of 20 m thickness were cut and mounted onto glass slides. Sections were placed in xylene for increasing lengths from 2 to 45 min. Acquired Raman spectra revealed the wax contribution in the fingerprint region until 28-min treatment in xylene. Good quality and wax-free spectra were recorded at 30, 35, 40, and 45 min in xylene with no significant differences among them. It is essential to collect uncontaminated Raman spectra in order to achieve authentic results because the fingerprint region of biological tissues holds extremely vital information that is of diagnostic significance in cancer. {\textcopyright} 2014 Taylor & Francis Group, LLC.",

keywords = "Analytical, Biological, Dewaxing protocol, Oral mucosa, Raman spectroscopy, Sample preparations, Dewaxing, Raman scattering, Xylene, Biological tissues, Glass slides, Sample preparation, Tissue sections, Tissue",

author = "S.A. Mian and H.E. Colley and M.H. Thornhill and I.U. Rehman",

year = "2014",

doi = "10.1080/05704928.2014.882348",

language = "English",

volume = "49",

pages = "614--617",

journal = "APPLIED SPECTROSCOPY REVIEWS",

issn = "0570-4928",

publisher = "Taylor and Francis Inc.",

number = "8",

}

RIS

TY - JOUR

T1 - Development of a dewaxing protocol for tissue-engineered models of the oral mucosa used for Raman spectroscopic analysis

AU - Mian, S.A.

AU - Colley, H.E.

AU - Thornhill, M.H.

AU - Rehman, I.U.

PY - 2014

Y1 - 2014

N2 - We demonstrate that wax-embedded models of tissue-engineered oral mucosa can be effectively dewaxed using xylene for Raman spectroscopic analysis. Tissue sections of 20 m thickness were cut and mounted onto glass slides. Sections were placed in xylene for increasing lengths from 2 to 45 min. Acquired Raman spectra revealed the wax contribution in the fingerprint region until 28-min treatment in xylene. Good quality and wax-free spectra were recorded at 30, 35, 40, and 45 min in xylene with no significant differences among them. It is essential to collect uncontaminated Raman spectra in order to achieve authentic results because the fingerprint region of biological tissues holds extremely vital information that is of diagnostic significance in cancer. © 2014 Taylor & Francis Group, LLC.

AB - We demonstrate that wax-embedded models of tissue-engineered oral mucosa can be effectively dewaxed using xylene for Raman spectroscopic analysis. Tissue sections of 20 m thickness were cut and mounted onto glass slides. Sections were placed in xylene for increasing lengths from 2 to 45 min. Acquired Raman spectra revealed the wax contribution in the fingerprint region until 28-min treatment in xylene. Good quality and wax-free spectra were recorded at 30, 35, 40, and 45 min in xylene with no significant differences among them. It is essential to collect uncontaminated Raman spectra in order to achieve authentic results because the fingerprint region of biological tissues holds extremely vital information that is of diagnostic significance in cancer. © 2014 Taylor & Francis Group, LLC.

KW - Analytical

KW - Biological

KW - Dewaxing protocol

KW - Oral mucosa

KW - Raman spectroscopy

KW - Sample preparations

KW - Dewaxing

KW - Raman scattering

KW - Xylene

KW - Biological tissues

KW - Glass slides

KW - Sample preparation

KW - Tissue sections

KW - Tissue

U2 - 10.1080/05704928.2014.882348

DO - 10.1080/05704928.2014.882348

M3 - Journal article

VL - 49

SP - 614

EP - 617

JO - APPLIED SPECTROSCOPY REVIEWS

JF - APPLIED SPECTROSCOPY REVIEWS

SN - 0570-4928

IS - 8

ER -

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