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Development of a genetically modified bacteriophage for use in tracing sources of pollution.

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Development of a genetically modified bacteriophage for use in tracing sources of pollution. / Daniell, T. J.; Davy, M. L.; Smith, R. J.
In: Journal of Applied Microbiology, Vol. 88, No. 5, 05.2000, p. 860-869.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Daniell, TJ, Davy, ML & Smith, RJ 2000, 'Development of a genetically modified bacteriophage for use in tracing sources of pollution.', Journal of Applied Microbiology, vol. 88, no. 5, pp. 860-869. https://doi.org/10.1046/j.1365-2672.2000.01028.x

APA

Daniell, T. J., Davy, M. L., & Smith, R. J. (2000). Development of a genetically modified bacteriophage for use in tracing sources of pollution. Journal of Applied Microbiology, 88(5), 860-869. https://doi.org/10.1046/j.1365-2672.2000.01028.x

Vancouver

Daniell TJ, Davy ML, Smith RJ. Development of a genetically modified bacteriophage for use in tracing sources of pollution. Journal of Applied Microbiology. 2000 May;88(5):860-869. doi: 10.1046/j.1365-2672.2000.01028.x

Author

Daniell, T. J. ; Davy, M. L. ; Smith, R. J. / Development of a genetically modified bacteriophage for use in tracing sources of pollution. In: Journal of Applied Microbiology. 2000 ; Vol. 88, No. 5. pp. 860-869.

Bibtex

@article{137e537080cb42ca94e086ea6d4c05b3,
title = "Development of a genetically modified bacteriophage for use in tracing sources of pollution.",
abstract = "Bacteriophage are frequently used as biotracers to identify the source of water pollutants. Genetic manipulation of bacteriophage M13mp18 has been used to enhance this technique by creating a library in which each recombinant bacteriophage genome contains a unique identification sequence. Techniques that identify a recombinant bacteriophage by the presence of the identification sequence, including polymerase chain reaction, restriction site polymorphism and plaque hybridization, have been developed. Recombinant bacteriophage can be used to test a large number of suspected sources simultaneously. The identification sequence also eliminates confusion with natural bacteriophage present in water samples. The performance of the modified bacteriophage and the techniques were assessed in simulated field trials on a restricted site carried out under a consent for environmental release of a genetically modified organism. The techniques were also field tested at sites in northwest England using wild-type M13 bacteriophage.",
author = "Daniell, {T. J.} and Davy, {M. L.} and Smith, {R. J.}",
year = "2000",
month = may,
doi = "10.1046/j.1365-2672.2000.01028.x",
language = "English",
volume = "88",
pages = "860--869",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "5",

}

RIS

TY - JOUR

T1 - Development of a genetically modified bacteriophage for use in tracing sources of pollution.

AU - Daniell, T. J.

AU - Davy, M. L.

AU - Smith, R. J.

PY - 2000/5

Y1 - 2000/5

N2 - Bacteriophage are frequently used as biotracers to identify the source of water pollutants. Genetic manipulation of bacteriophage M13mp18 has been used to enhance this technique by creating a library in which each recombinant bacteriophage genome contains a unique identification sequence. Techniques that identify a recombinant bacteriophage by the presence of the identification sequence, including polymerase chain reaction, restriction site polymorphism and plaque hybridization, have been developed. Recombinant bacteriophage can be used to test a large number of suspected sources simultaneously. The identification sequence also eliminates confusion with natural bacteriophage present in water samples. The performance of the modified bacteriophage and the techniques were assessed in simulated field trials on a restricted site carried out under a consent for environmental release of a genetically modified organism. The techniques were also field tested at sites in northwest England using wild-type M13 bacteriophage.

AB - Bacteriophage are frequently used as biotracers to identify the source of water pollutants. Genetic manipulation of bacteriophage M13mp18 has been used to enhance this technique by creating a library in which each recombinant bacteriophage genome contains a unique identification sequence. Techniques that identify a recombinant bacteriophage by the presence of the identification sequence, including polymerase chain reaction, restriction site polymorphism and plaque hybridization, have been developed. Recombinant bacteriophage can be used to test a large number of suspected sources simultaneously. The identification sequence also eliminates confusion with natural bacteriophage present in water samples. The performance of the modified bacteriophage and the techniques were assessed in simulated field trials on a restricted site carried out under a consent for environmental release of a genetically modified organism. The techniques were also field tested at sites in northwest England using wild-type M13 bacteriophage.

U2 - 10.1046/j.1365-2672.2000.01028.x

DO - 10.1046/j.1365-2672.2000.01028.x

M3 - Journal article

VL - 88

SP - 860

EP - 869

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 5

ER -