Home > Research > Publications & Outputs > Development of Leishmania orientalis in the san...

Electronic data

  • Chanmol_et_al_AAM

    Rights statement: This is the author’s version of a work that was accepted for publication in Acta Tropica. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Acta Tropica, 199, 2019 DOI: 10.1016/j.actatropica.2019.105157

    Accepted author manuscript, 292 KB, PDF document

    Available under license: CC BY-NC-ND

Links

Text available via DOI:

View graph of relations

Development of Leishmania orientalis in the sand fly Lutzomyia longipalpis (Diptera: Psychodidae) and the biting midge Culicoides soronensis (Diptera: Ceratopogonidae)

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

Development of Leishmania orientalis in the sand fly Lutzomyia longipalpis (Diptera: Psychodidae) and the biting midge Culicoides soronensis (Diptera: Ceratopogonidae). / Chanmol, Wetpisit; Jariyapan, Narissara; Somboon, Pradya et al.

In: Acta Tropica, Vol. 199, 105157, 01.11.2019.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

APA

Vancouver

Chanmol W, Jariyapan N, Somboon P, Bates MD, Bates PA. Development of Leishmania orientalis in the sand fly Lutzomyia longipalpis (Diptera: Psychodidae) and the biting midge Culicoides soronensis (Diptera: Ceratopogonidae). Acta Tropica. 2019 Nov 1;199:105157. Epub 2019 Sep 3. doi: 10.1016/j.actatropica.2019.105157

Author

Bibtex

@article{c1790c44e5f848b1ad3a2198a0dd46f6,
title = "Development of Leishmania orientalis in the sand fly Lutzomyia longipalpis (Diptera: Psychodidae) and the biting midge Culicoides soronensis (Diptera: Ceratopogonidae)",
abstract = "Leishmania (Mundinia) orientalis is a newly described species causing human leishmaniasis in Thailand whose natural vector is unknown. L. orientalis infections in sand flies and/or biting midges under laboratory conditions have not been previously investigated. In this study, the development of L. orientalis in two experimental vectors, Lutzomyia longipalpis sand flies and Culicoides sonorensis biting midges was investigated for the first time using light microscopy, scanning electron microscopy, and histological examination. The results showed that L. orientalis was unable to establish infection in Lu. longipalpis. No parasites were found in the sand fly gut 4 days post-infected blood meal (PIBM). In contrast, the parasite successfully established infection in C. sonorensis. The parasites differentiated from amastigotes to procyclic promastigotes in the abdominal midgut (AMG) on day 1 PIBM. On day 2 PIBM, nectomonad promastigotes were observed in the AMG and migrated to the thoracic midgut (TMG). Leptomonad promastigotes appeared at the TMG on day 3 PIBM. Clusters of leptomonad promastigotes and metacyclic promastigotes colonized around the stomodeal valve with the accumulation of a promastigote secretory gel-like material from day 3 PIBM onwards. Haptomonad-like promastigotes were observed from day 5 PIBM, and the proportion of metacyclic promastigotes reached 23% on day 7 PIBM. The results suggest that biting midges or other sand fly genera or species might be vectors of L. orientalis.",
keywords = "Leishmania orientalis, Mundinia, Lutzomyia longipalpis, Culicoides sonorensis, Leishmaniasis, Thailand",
author = "Wetpisit Chanmol and Narissara Jariyapan and Pradya Somboon and Bates, {Michelle D} and Bates, {Paul A}",
note = "This is the author{\textquoteright}s version of a work that was accepted for publication in Acta Tropica. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Acta Tropica, 199, 2019 DOI: 10.1016/j.actatropica.2019.105157",
year = "2019",
month = nov,
day = "1",
doi = "10.1016/j.actatropica.2019.105157",
language = "English",
volume = "199",
journal = "Acta Tropica",
issn = "0001-706X",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Development of Leishmania orientalis in the sand fly Lutzomyia longipalpis (Diptera: Psychodidae) and the biting midge Culicoides soronensis (Diptera: Ceratopogonidae)

AU - Chanmol, Wetpisit

AU - Jariyapan, Narissara

AU - Somboon, Pradya

AU - Bates, Michelle D

AU - Bates, Paul A

N1 - This is the author’s version of a work that was accepted for publication in Acta Tropica. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Acta Tropica, 199, 2019 DOI: 10.1016/j.actatropica.2019.105157

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Leishmania (Mundinia) orientalis is a newly described species causing human leishmaniasis in Thailand whose natural vector is unknown. L. orientalis infections in sand flies and/or biting midges under laboratory conditions have not been previously investigated. In this study, the development of L. orientalis in two experimental vectors, Lutzomyia longipalpis sand flies and Culicoides sonorensis biting midges was investigated for the first time using light microscopy, scanning electron microscopy, and histological examination. The results showed that L. orientalis was unable to establish infection in Lu. longipalpis. No parasites were found in the sand fly gut 4 days post-infected blood meal (PIBM). In contrast, the parasite successfully established infection in C. sonorensis. The parasites differentiated from amastigotes to procyclic promastigotes in the abdominal midgut (AMG) on day 1 PIBM. On day 2 PIBM, nectomonad promastigotes were observed in the AMG and migrated to the thoracic midgut (TMG). Leptomonad promastigotes appeared at the TMG on day 3 PIBM. Clusters of leptomonad promastigotes and metacyclic promastigotes colonized around the stomodeal valve with the accumulation of a promastigote secretory gel-like material from day 3 PIBM onwards. Haptomonad-like promastigotes were observed from day 5 PIBM, and the proportion of metacyclic promastigotes reached 23% on day 7 PIBM. The results suggest that biting midges or other sand fly genera or species might be vectors of L. orientalis.

AB - Leishmania (Mundinia) orientalis is a newly described species causing human leishmaniasis in Thailand whose natural vector is unknown. L. orientalis infections in sand flies and/or biting midges under laboratory conditions have not been previously investigated. In this study, the development of L. orientalis in two experimental vectors, Lutzomyia longipalpis sand flies and Culicoides sonorensis biting midges was investigated for the first time using light microscopy, scanning electron microscopy, and histological examination. The results showed that L. orientalis was unable to establish infection in Lu. longipalpis. No parasites were found in the sand fly gut 4 days post-infected blood meal (PIBM). In contrast, the parasite successfully established infection in C. sonorensis. The parasites differentiated from amastigotes to procyclic promastigotes in the abdominal midgut (AMG) on day 1 PIBM. On day 2 PIBM, nectomonad promastigotes were observed in the AMG and migrated to the thoracic midgut (TMG). Leptomonad promastigotes appeared at the TMG on day 3 PIBM. Clusters of leptomonad promastigotes and metacyclic promastigotes colonized around the stomodeal valve with the accumulation of a promastigote secretory gel-like material from day 3 PIBM onwards. Haptomonad-like promastigotes were observed from day 5 PIBM, and the proportion of metacyclic promastigotes reached 23% on day 7 PIBM. The results suggest that biting midges or other sand fly genera or species might be vectors of L. orientalis.

KW - Leishmania orientalis

KW - Mundinia

KW - Lutzomyia longipalpis

KW - Culicoides sonorensis

KW - Leishmaniasis

KW - Thailand

U2 - 10.1016/j.actatropica.2019.105157

DO - 10.1016/j.actatropica.2019.105157

M3 - Journal article

C2 - 31491400

VL - 199

JO - Acta Tropica

JF - Acta Tropica

SN - 0001-706X

M1 - 105157

ER -