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Development of temporal temperature gradient electrophoresis for characterising methanogen diversity.

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Development of temporal temperature gradient electrophoresis for characterising methanogen diversity. / Earl, Julie; Pickup, Roger W.; Ritchie, Donald A. et al.
In: Microbial Ecology, Vol. 50, No. 3, 24.11.2005, p. 327-336.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Earl, J, Pickup, RW, Ritchie, DA & Edwards, C 2005, 'Development of temporal temperature gradient electrophoresis for characterising methanogen diversity.', Microbial Ecology, vol. 50, no. 3, pp. 327-336. https://doi.org/10.1007/s00248-005-0192-1

APA

Earl, J., Pickup, R. W., Ritchie, D. A., & Edwards, C. (2005). Development of temporal temperature gradient electrophoresis for characterising methanogen diversity. Microbial Ecology, 50(3), 327-336. https://doi.org/10.1007/s00248-005-0192-1

Vancouver

Earl J, Pickup RW, Ritchie DA, Edwards C. Development of temporal temperature gradient electrophoresis for characterising methanogen diversity. Microbial Ecology. 2005 Nov 24;50(3):327-336. doi: 10.1007/s00248-005-0192-1

Author

Earl, Julie ; Pickup, Roger W. ; Ritchie, Donald A. et al. / Development of temporal temperature gradient electrophoresis for characterising methanogen diversity. In: Microbial Ecology. 2005 ; Vol. 50, No. 3. pp. 327-336.

Bibtex

@article{05b8bffa48d94111a9edc74ec2fbc0c0,
title = "Development of temporal temperature gradient electrophoresis for characterising methanogen diversity.",
abstract = "Temporal temperature gradient electrophoretic (TTGE) analysis of 16S rDNA sequences was optimized to monitor the methanogen population present in water and sediments of a small eutrophic lake, Priest Pot, in the English Lake district. The production of nonrepresentative TTGE profiles due to the generation of polymerase chain reaction (PCR) artifacts initially proved problematical. The use of a proofreading polymerase in the PCR was found to be essential and fully optimized protocols were established and tested to ensure confidence that the TTGE profiles truly reflected sequence diversity. TTGE analysis revealed the methanogen population to be less diverse in water than in sediment. The most genetic diversity was observed in TTGE profiles of sediment DNA isolated in winter and the least was in sediment DNA isolated in summer. DNA sequencing analysis of bands recovered from TTGE gels revealed the presence of two methanogen communities. One clustered with Methanosaeta species and the other with the Methanomicrobiales. Many sequences showed low DNA sequence similarity to known methanogens, suggesting that Priest Pot harbors previously undescribed methanogen species.",
author = "Julie Earl and Pickup, {Roger W.} and Ritchie, {Donald A.} and Clive Edwards",
year = "2005",
month = nov,
day = "24",
doi = "10.1007/s00248-005-0192-1",
language = "English",
volume = "50",
pages = "327--336",
journal = "Microbial Ecology",
issn = "0095-3628",
publisher = "Springer New York",
number = "3",

}

RIS

TY - JOUR

T1 - Development of temporal temperature gradient electrophoresis for characterising methanogen diversity.

AU - Earl, Julie

AU - Pickup, Roger W.

AU - Ritchie, Donald A.

AU - Edwards, Clive

PY - 2005/11/24

Y1 - 2005/11/24

N2 - Temporal temperature gradient electrophoretic (TTGE) analysis of 16S rDNA sequences was optimized to monitor the methanogen population present in water and sediments of a small eutrophic lake, Priest Pot, in the English Lake district. The production of nonrepresentative TTGE profiles due to the generation of polymerase chain reaction (PCR) artifacts initially proved problematical. The use of a proofreading polymerase in the PCR was found to be essential and fully optimized protocols were established and tested to ensure confidence that the TTGE profiles truly reflected sequence diversity. TTGE analysis revealed the methanogen population to be less diverse in water than in sediment. The most genetic diversity was observed in TTGE profiles of sediment DNA isolated in winter and the least was in sediment DNA isolated in summer. DNA sequencing analysis of bands recovered from TTGE gels revealed the presence of two methanogen communities. One clustered with Methanosaeta species and the other with the Methanomicrobiales. Many sequences showed low DNA sequence similarity to known methanogens, suggesting that Priest Pot harbors previously undescribed methanogen species.

AB - Temporal temperature gradient electrophoretic (TTGE) analysis of 16S rDNA sequences was optimized to monitor the methanogen population present in water and sediments of a small eutrophic lake, Priest Pot, in the English Lake district. The production of nonrepresentative TTGE profiles due to the generation of polymerase chain reaction (PCR) artifacts initially proved problematical. The use of a proofreading polymerase in the PCR was found to be essential and fully optimized protocols were established and tested to ensure confidence that the TTGE profiles truly reflected sequence diversity. TTGE analysis revealed the methanogen population to be less diverse in water than in sediment. The most genetic diversity was observed in TTGE profiles of sediment DNA isolated in winter and the least was in sediment DNA isolated in summer. DNA sequencing analysis of bands recovered from TTGE gels revealed the presence of two methanogen communities. One clustered with Methanosaeta species and the other with the Methanomicrobiales. Many sequences showed low DNA sequence similarity to known methanogens, suggesting that Priest Pot harbors previously undescribed methanogen species.

U2 - 10.1007/s00248-005-0192-1

DO - 10.1007/s00248-005-0192-1

M3 - Journal article

C2 - 16328656

AN - SCOPUS:33645073945

VL - 50

SP - 327

EP - 336

JO - Microbial Ecology

JF - Microbial Ecology

SN - 0095-3628

IS - 3

ER -