Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Developmental effects of over-expression of normal and mutated forms of a Xenopus NF-κB homologue
AU - Richardson, Jill C.
AU - Gatherer, Derek
AU - Woodland, Hugh R.
PY - 1995/8
Y1 - 1995/8
N2 - High level over-expression of XrelA1, a homologue of the p65 sub-unit of NF-kappa B and of Drosophila dorsal, arrests Xenopus development at the gastrula stage, producing a reduction in the levels of expression of various genes of developmental interest without general reduction in transcription or cessation of cell division. There is little Goosecoid expression, even though a dorsal lip forms. At lower levels XrelA1 mRNA primarily produces disruption of the mid-dorsal axis. A dominant interference gene product, Delta 222, produces mainly posterior, but also anterior abnormalities. On the basis of these results we postulate that the role of XrelA1 in the vertebrate embryo is unlikely to be in dorsoventral development, but more likely in the formation of the termini.
AB - High level over-expression of XrelA1, a homologue of the p65 sub-unit of NF-kappa B and of Drosophila dorsal, arrests Xenopus development at the gastrula stage, producing a reduction in the levels of expression of various genes of developmental interest without general reduction in transcription or cessation of cell division. There is little Goosecoid expression, even though a dorsal lip forms. At lower levels XrelA1 mRNA primarily produces disruption of the mid-dorsal axis. A dominant interference gene product, Delta 222, produces mainly posterior, but also anterior abnormalities. On the basis of these results we postulate that the role of XrelA1 in the vertebrate embryo is unlikely to be in dorsoventral development, but more likely in the formation of the termini.
KW - XENOPUS
KW - NF-KAPPA-B
KW - DORSAL
KW - AXIS FORMATION
KW - DROSOPHILA EMBRYO
KW - DORSOVENTRAL PATTERN
KW - MESODERM INDUCTION
KW - NEURAL INDUCTION
KW - DORSAL PROTEIN
KW - ORGANIZER
KW - CACTUS
KW - LAEVIS
KW - SPECIFICATION
KW - MORPHOGEN
U2 - 10.1016/0925-4773(95)00388-H
DO - 10.1016/0925-4773(95)00388-H
M3 - Journal article
VL - 52
SP - 165
EP - 177
JO - Mechanisms of Development
JF - Mechanisms of Development
SN - 0925-4773
IS - 2-3
ER -