TY - JOUR

T1 - Diagnose Pathogens in Drinking Water via Magnetic Surface-Enhanced Raman Scattering (SERS) Assay

AU - Li, Hanbing

AU - Li, Cui

AU - Martin, Francis L.

AU - Zhang, Dayi

N1 - This is the author’s version of a work that was accepted for publication in Materials Today Proceedings. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Materials Today Proceedings, 4, 1, 2017 DOI: 10.1016/j.matpr.2017.01.189

PY - 2017/3/19

Y1 - 2017/3/19

N2 - Abstract Rapid identification and diagnosis of bacteria and other microorganisms is a great challenge for drinking water safety due to the increasing frequency of pathogenic infections. Raman spectroscopy is a non-destructive tool to characterize the biochemical fingerprints of bacterial cells and its signal can be improved by surface-enhanced Raman scattering (SERS). Thus, Raman scattering has a huge potential in fast diagnosis of pathogens in drinking water, with low cost and high reproducibility. In this work, we developed a novel fast diagnosis method to detect aquatic pathogens via magnetic SERS assay. With chemical co-precipitation synthesis and surface glucose reduction, the silver-coated magnetic nanoparticles (Ag@MNPs) had a well-developed core-shell structure and high efficiency to capture bacterial cells. Ag@MNPs achieved 103 enhancement factor for rhodamine 6G and the limit of detection was 10-9 M. The magnetic SERS assay also successfully detected various bacteria (A. baylyi and E. coli) with high sensitivity (105 CFU/mL). This platform provided a promising and easy-operation approach for pathogen detection for food and drinking water safety.

AB - Abstract Rapid identification and diagnosis of bacteria and other microorganisms is a great challenge for drinking water safety due to the increasing frequency of pathogenic infections. Raman spectroscopy is a non-destructive tool to characterize the biochemical fingerprints of bacterial cells and its signal can be improved by surface-enhanced Raman scattering (SERS). Thus, Raman scattering has a huge potential in fast diagnosis of pathogens in drinking water, with low cost and high reproducibility. In this work, we developed a novel fast diagnosis method to detect aquatic pathogens via magnetic SERS assay. With chemical co-precipitation synthesis and surface glucose reduction, the silver-coated magnetic nanoparticles (Ag@MNPs) had a well-developed core-shell structure and high efficiency to capture bacterial cells. Ag@MNPs achieved 103 enhancement factor for rhodamine 6G and the limit of detection was 10-9 M. The magnetic SERS assay also successfully detected various bacteria (A. baylyi and E. coli) with high sensitivity (105 CFU/mL). This platform provided a promising and easy-operation approach for pathogen detection for food and drinking water safety.

KW - Magnetic nanoparticles (MNPs)

KW - Silver-coated MNPs (Ag@MNPs)

KW - Raman spectroscopy

KW - Surface-enhanced Raman scattering (SERS)

U2 - 10.1016/j.matpr.2017.01.189

DO - 10.1016/j.matpr.2017.01.189

M3 - Journal article

VL - 4

SP - 25

EP - 31

JO - Materials Today: Proceedings

JF - Materials Today: Proceedings

SN - 2214-7853

IS - 1

ER -