Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Direct effects of snowdrop lectin (GNA) on larvae of three aphid predators and fate of GNA after ingestion.
AU - Hogervorst, Petra A. M.
AU - Ferry, Natalie
AU - Gatehouse, Angharad M. R.
AU - Wäckers, Felix L.
AU - Romeis, Jörg
PY - 2006/4
Y1 - 2006/4
N2 - Plants genetically modified to express Galanthus nivalis agglutinin (GNA) have been found to confer partial resistance to homopteran pests. Laboratory experiments were conducted to investigate direct effects of GNA on larvae of three species of aphid predators that differ in their feeding and digestive physiology, i.e. Chrysoperla carnea, Adalia bipunctata and Coccinella septempunctata. Longevity of all three predator species was directly affected by GNA, when they were fed a sucrose solution containing 1% GNA. However, a difference in sensitivity towards GNA was observed when comparing the first and last larval stage of the three species. In vitro studies revealed that gut enzymes from none of the three species were able to break down GNA. In vivo feed-chase studies demonstrated accumulation of GNA in the larvae. After the larvae had been transferred to a diet devoid of GNA, the protein stayed present in the body of C. carnea, but decreased over time in both ladybirds. Binding studies showed that GNA binds to glycoproteins that can be found in the guts of larvae of all three predator species. Immunoassay by Western blotting of haemolymph samples only occasionally showed the presence of GNA. Fluorescence microscopy confirmed GNA accumulation in the midgut of C. carnea larvae. Implications of these findings for non-target risk assessment of GNA-transgenic crops are discussed.
AB - Plants genetically modified to express Galanthus nivalis agglutinin (GNA) have been found to confer partial resistance to homopteran pests. Laboratory experiments were conducted to investigate direct effects of GNA on larvae of three species of aphid predators that differ in their feeding and digestive physiology, i.e. Chrysoperla carnea, Adalia bipunctata and Coccinella septempunctata. Longevity of all three predator species was directly affected by GNA, when they were fed a sucrose solution containing 1% GNA. However, a difference in sensitivity towards GNA was observed when comparing the first and last larval stage of the three species. In vitro studies revealed that gut enzymes from none of the three species were able to break down GNA. In vivo feed-chase studies demonstrated accumulation of GNA in the larvae. After the larvae had been transferred to a diet devoid of GNA, the protein stayed present in the body of C. carnea, but decreased over time in both ladybirds. Binding studies showed that GNA binds to glycoproteins that can be found in the guts of larvae of all three predator species. Immunoassay by Western blotting of haemolymph samples only occasionally showed the presence of GNA. Fluorescence microscopy confirmed GNA accumulation in the midgut of C. carnea larvae. Implications of these findings for non-target risk assessment of GNA-transgenic crops are discussed.
KW - Galanthus nivalis agglutinin
KW - Chrysoperla carnea
KW - Adalia bipunctata
KW - Coccinella septempunctata
KW - Non-target effects
KW - Risk assessment
U2 - 10.1016/j.jinsphys.2006.02.011
DO - 10.1016/j.jinsphys.2006.02.011
M3 - Journal article
VL - 52
SP - 614
EP - 624
JO - Journal of Insect Physiology
JF - Journal of Insect Physiology
SN - 0022-1910
IS - 6
ER -