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Direct phenotypic and genotypic detection of a recombinant pseudomonad population released into lake water

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

Direct phenotypic and genotypic detection of a recombinant pseudomonad population released into lake water. / Morgan, J. A.W.; Winstanley, C.; Pickup, R. W. et al.
In: Applied and Environmental Microbiology, Vol. 55, No. 10, 01.10.1989, p. 2537-2544.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Morgan, JAW, Winstanley, C, Pickup, RW, Jones, JG & Saunders, JR 1989, 'Direct phenotypic and genotypic detection of a recombinant pseudomonad population released into lake water', Applied and Environmental Microbiology, vol. 55, no. 10, pp. 2537-2544. https://doi.org/10.1128/aem.55.10.2537-2544.1989

APA

Morgan, J. A. W., Winstanley, C., Pickup, R. W., Jones, J. G., & Saunders, J. R. (1989). Direct phenotypic and genotypic detection of a recombinant pseudomonad population released into lake water. Applied and Environmental Microbiology, 55(10), 2537-2544. https://doi.org/10.1128/aem.55.10.2537-2544.1989

Vancouver

Morgan JAW, Winstanley C, Pickup RW, Jones JG, Saunders JR. Direct phenotypic and genotypic detection of a recombinant pseudomonad population released into lake water. Applied and Environmental Microbiology. 1989 Oct 1;55(10):2537-2544. doi: 10.1128/aem.55.10.2537-2544.1989

Author

Morgan, J. A.W. ; Winstanley, C. ; Pickup, R. W. et al. / Direct phenotypic and genotypic detection of a recombinant pseudomonad population released into lake water. In: Applied and Environmental Microbiology. 1989 ; Vol. 55, No. 10. pp. 2537-2544.

Bibtex

@article{566b917f35384d73846b9fba2169f751,
title = "Direct phenotypic and genotypic detection of a recombinant pseudomonad population released into lake water",
abstract = "As a system for studying the fate of genetically engineered microorganisms in the environment, we have previously constructed recombinant plasmids encoding a xylE marker gene (C. Winstanley, J.A.W. Morgan, R.W. Pickup, J.G. Jones, and J.R. Saunders, Appl. Environ. Microbiol. 55: 771-777, 1989). A series of direct membrane filter methods have been developed which facilitate the detection of bacterial cells harboring the xylE gene, its product, catechol 2,3-dioxygenase, and catechol 2,3-dioxygenase enzyme activity directly from water samples. These methods enable detection of recombinant populations at concentrations as low as 103 to 104 cells ml of lake water-1. Direct detection facilitates ecological studies of a range of bacterial strains containing the marker system in aquatic environments. The fate of a recombinant pseudomonad population in lake water was assessed by a combination of colony-forming ability, direct counts, and direct detection of the xylE gene and phenotypic expression of its product.",
author = "Morgan, {J. A.W.} and C. Winstanley and Pickup, {R. W.} and Jones, {J. G.} and Saunders, {J. R.}",
year = "1989",
month = oct,
day = "1",
doi = "10.1128/aem.55.10.2537-2544.1989",
language = "English",
volume = "55",
pages = "2537--2544",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "10",

}

RIS

TY - JOUR

T1 - Direct phenotypic and genotypic detection of a recombinant pseudomonad population released into lake water

AU - Morgan, J. A.W.

AU - Winstanley, C.

AU - Pickup, R. W.

AU - Jones, J. G.

AU - Saunders, J. R.

PY - 1989/10/1

Y1 - 1989/10/1

N2 - As a system for studying the fate of genetically engineered microorganisms in the environment, we have previously constructed recombinant plasmids encoding a xylE marker gene (C. Winstanley, J.A.W. Morgan, R.W. Pickup, J.G. Jones, and J.R. Saunders, Appl. Environ. Microbiol. 55: 771-777, 1989). A series of direct membrane filter methods have been developed which facilitate the detection of bacterial cells harboring the xylE gene, its product, catechol 2,3-dioxygenase, and catechol 2,3-dioxygenase enzyme activity directly from water samples. These methods enable detection of recombinant populations at concentrations as low as 103 to 104 cells ml of lake water-1. Direct detection facilitates ecological studies of a range of bacterial strains containing the marker system in aquatic environments. The fate of a recombinant pseudomonad population in lake water was assessed by a combination of colony-forming ability, direct counts, and direct detection of the xylE gene and phenotypic expression of its product.

AB - As a system for studying the fate of genetically engineered microorganisms in the environment, we have previously constructed recombinant plasmids encoding a xylE marker gene (C. Winstanley, J.A.W. Morgan, R.W. Pickup, J.G. Jones, and J.R. Saunders, Appl. Environ. Microbiol. 55: 771-777, 1989). A series of direct membrane filter methods have been developed which facilitate the detection of bacterial cells harboring the xylE gene, its product, catechol 2,3-dioxygenase, and catechol 2,3-dioxygenase enzyme activity directly from water samples. These methods enable detection of recombinant populations at concentrations as low as 103 to 104 cells ml of lake water-1. Direct detection facilitates ecological studies of a range of bacterial strains containing the marker system in aquatic environments. The fate of a recombinant pseudomonad population in lake water was assessed by a combination of colony-forming ability, direct counts, and direct detection of the xylE gene and phenotypic expression of its product.

U2 - 10.1128/aem.55.10.2537-2544.1989

DO - 10.1128/aem.55.10.2537-2544.1989

M3 - Journal article

C2 - 2604395

AN - SCOPUS:0024432497

VL - 55

SP - 2537

EP - 2544

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 10

ER -