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Dissection of the ozone-induced calcium signature.

Research output: Contribution to journalJournal articlepeer-review

  • Helen Clayton
  • Marc R. Knight
  • Heather Knight
  • Martin McAinsh
  • Alistair M. Hetherington
<mark>Journal publication date</mark>03/1999
<mark>Journal</mark>Plant Journal
Issue number5
Number of pages5
Pages (from-to)575-579
Publication StatusPublished
<mark>Original language</mark>English


The cellular responses of plants to numerous extracellular stimuli are mediated by transient elevations in the concentration of cytosolic free calcium ([Ca2+]cyt). We have addressed the question of how cells can use this apparently ubiquitous system to initiate so many specific and appropriate end responses. We show that the pollutant gas ozone elicits a biphasic Ca2+ response in intact Arabidopsis plants and a subsequent increase in expression of the gene encoding the antioxidant defence enzyme glutathione-S-transferase (GST). The second of the two [Ca2+]cyt peaks, but not the first, could be eliminated either by pre-treatment of plants with lanthanum chloride, or by reducing the duration of ozone fumigation. Under these conditions, ozone-inducedGSTexpression was abolished. These data provide a functional dissection of the ozone Ca2+ signalling pathway and indicate that the second ozone-induced [Ca2+]cyt peak provides the necessary information to direct expression of GST.