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Diversity of phage integrases in Enterobacteriaceae: Development of markers for environmental analysis of temperate phages

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Diversity of phage integrases in Enterobacteriaceae: Development of markers for environmental analysis of temperate phages. / Balding, Claire; Bromley, Stephen A.; Pickup, Roger W. et al.
In: Environmental Microbiology, Vol. 7, No. 10, 31.10.2005, p. 1558-1567.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Balding, C, Bromley, SA, Pickup, RW & Saunders, JR 2005, 'Diversity of phage integrases in Enterobacteriaceae: Development of markers for environmental analysis of temperate phages', Environmental Microbiology, vol. 7, no. 10, pp. 1558-1567. https://doi.org/10.1111/j.1462-2920.2005.00845.x

APA

Balding, C., Bromley, S. A., Pickup, R. W., & Saunders, J. R. (2005). Diversity of phage integrases in Enterobacteriaceae: Development of markers for environmental analysis of temperate phages. Environmental Microbiology, 7(10), 1558-1567. https://doi.org/10.1111/j.1462-2920.2005.00845.x

Vancouver

Balding C, Bromley SA, Pickup RW, Saunders JR. Diversity of phage integrases in Enterobacteriaceae: Development of markers for environmental analysis of temperate phages. Environmental Microbiology. 2005 Oct 31;7(10):1558-1567. doi: 10.1111/j.1462-2920.2005.00845.x

Author

Balding, Claire ; Bromley, Stephen A. ; Pickup, Roger W. et al. / Diversity of phage integrases in Enterobacteriaceae : Development of markers for environmental analysis of temperate phages. In: Environmental Microbiology. 2005 ; Vol. 7, No. 10. pp. 1558-1567.

Bibtex

@article{cb2527c58373468395f90ccdc5c399c3,
title = "Diversity of phage integrases in Enterobacteriaceae: Development of markers for environmental analysis of temperate phages",
abstract = "Viruses are the most abundant biological entities in aquatic systems. Temperate bacteriophages hav e enormous influences on microbial diversity, genetic exchange and bacterial population dynamics. However, development of molecular tools for their detection in the environment has been problematic. The integrase gene is used here as a molecular marker to analyse the diversity of temperate bacteriophages in a population of freshwater bacteria. Interrogation of the GenBank database revealed 32 non-cryptic enteric phage integrase sequences, leading to the development of a suite of 11 degenerate primer sets specific to the extant sequences elucidated. Application of these primer sets to enterobacterial isolates recovered from a freshwater pond and the temperate phages induced from them revealed a number of diverse integrase genes, including novel integrase-like sequences not represented in the databases. This highlights the potential of utilizing the integrase gene family as a marker for phage diversity.",
author = "Claire Balding and Bromley, {Stephen A.} and Pickup, {Roger W.} and Saunders, {Jon R.}",
year = "2005",
month = oct,
day = "31",
doi = "10.1111/j.1462-2920.2005.00845.x",
language = "English",
volume = "7",
pages = "1558--1567",
journal = "Environmental Microbiology",
issn = "1462-2912",
publisher = "Wiley-Blackwell",
number = "10",

}

RIS

TY - JOUR

T1 - Diversity of phage integrases in Enterobacteriaceae

T2 - Development of markers for environmental analysis of temperate phages

AU - Balding, Claire

AU - Bromley, Stephen A.

AU - Pickup, Roger W.

AU - Saunders, Jon R.

PY - 2005/10/31

Y1 - 2005/10/31

N2 - Viruses are the most abundant biological entities in aquatic systems. Temperate bacteriophages hav e enormous influences on microbial diversity, genetic exchange and bacterial population dynamics. However, development of molecular tools for their detection in the environment has been problematic. The integrase gene is used here as a molecular marker to analyse the diversity of temperate bacteriophages in a population of freshwater bacteria. Interrogation of the GenBank database revealed 32 non-cryptic enteric phage integrase sequences, leading to the development of a suite of 11 degenerate primer sets specific to the extant sequences elucidated. Application of these primer sets to enterobacterial isolates recovered from a freshwater pond and the temperate phages induced from them revealed a number of diverse integrase genes, including novel integrase-like sequences not represented in the databases. This highlights the potential of utilizing the integrase gene family as a marker for phage diversity.

AB - Viruses are the most abundant biological entities in aquatic systems. Temperate bacteriophages hav e enormous influences on microbial diversity, genetic exchange and bacterial population dynamics. However, development of molecular tools for their detection in the environment has been problematic. The integrase gene is used here as a molecular marker to analyse the diversity of temperate bacteriophages in a population of freshwater bacteria. Interrogation of the GenBank database revealed 32 non-cryptic enteric phage integrase sequences, leading to the development of a suite of 11 degenerate primer sets specific to the extant sequences elucidated. Application of these primer sets to enterobacterial isolates recovered from a freshwater pond and the temperate phages induced from them revealed a number of diverse integrase genes, including novel integrase-like sequences not represented in the databases. This highlights the potential of utilizing the integrase gene family as a marker for phage diversity.

U2 - 10.1111/j.1462-2920.2005.00845.x

DO - 10.1111/j.1462-2920.2005.00845.x

M3 - Journal article

C2 - 16156729

AN - SCOPUS:25144455626

VL - 7

SP - 1558

EP - 1567

JO - Environmental Microbiology

JF - Environmental Microbiology

SN - 1462-2912

IS - 10

ER -