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DNA polymerase B is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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DNA polymerase B is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts. / Allinson, Sarah L.; Dianova, Irina I.; Dianov, Grigory L.
In: EMBO Journal, Vol. 20, No. 23, 03.12.2001, p. 6919-6926.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Allinson, SL, Dianova, II & Dianov, GL 2001, 'DNA polymerase B is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts', EMBO Journal, vol. 20, no. 23, pp. 6919-6926. https://doi.org/10.1093/emboj/20.23.6919

APA

Allinson, S. L., Dianova, I. I., & Dianov, G. L. (2001). DNA polymerase B is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts. EMBO Journal, 20(23), 6919-6926. https://doi.org/10.1093/emboj/20.23.6919

Vancouver

Allinson SL, Dianova II, Dianov GL. DNA polymerase B is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts. EMBO Journal. 2001 Dec 3;20(23):6919-6926. doi: 10.1093/emboj/20.23.6919

Author

Allinson, Sarah L. ; Dianova, Irina I. ; Dianov, Grigory L. / DNA polymerase B is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts. In: EMBO Journal. 2001 ; Vol. 20, No. 23. pp. 6919-6926.

Bibtex

@article{bb8cf39619ab45ebaa0edf88a33bc8bd,
title = "DNA polymerase B is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts",
abstract = "The repair of oxidative base lesions in DNA is a coordinated chain of reactions that includes removal of the damaged base, incision of the phosphodiester backbone at the abasic sugar residue, incorporation of an undamaged nucleotide and sealing of the DNA strand break. Although removal of a damaged base in mammalian cells is initiated primarily by a damage-specific DNA glycosylase, several lyases and DNA polymerases may contribute to the later stages of repair. DNA polymerase (Pol ) was implicated recently as the major polymerase involved in repair of oxidative base lesions; however, the identity of the lyase participating in the repair of oxidative lesions is unclear. We studied the mechanism by which mammalian cell extracts process DNA substrates containing a single 8-oxoguanine or 5,6-dihydrouracil at a defined position. We find that, when repair synthesis proceeds through a Pol -dependent single nucleotide replacement mechanism, the 5'-deoxyribosephosphate lyase activity of Pol is essential for repair of both lesions.",
keywords = "base excision repair, cell extracts, DNA polymerase, NTH1, OGG1",
author = "Allinson, {Sarah L.} and Dianova, {Irina I.} and Dianov, {Grigory L.}",
year = "2001",
month = dec,
day = "3",
doi = "10.1093/emboj/20.23.6919",
language = "English",
volume = "20",
pages = "6919--6926",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "23",

}

RIS

TY - JOUR

T1 - DNA polymerase B is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts

AU - Allinson, Sarah L.

AU - Dianova, Irina I.

AU - Dianov, Grigory L.

PY - 2001/12/3

Y1 - 2001/12/3

N2 - The repair of oxidative base lesions in DNA is a coordinated chain of reactions that includes removal of the damaged base, incision of the phosphodiester backbone at the abasic sugar residue, incorporation of an undamaged nucleotide and sealing of the DNA strand break. Although removal of a damaged base in mammalian cells is initiated primarily by a damage-specific DNA glycosylase, several lyases and DNA polymerases may contribute to the later stages of repair. DNA polymerase (Pol ) was implicated recently as the major polymerase involved in repair of oxidative base lesions; however, the identity of the lyase participating in the repair of oxidative lesions is unclear. We studied the mechanism by which mammalian cell extracts process DNA substrates containing a single 8-oxoguanine or 5,6-dihydrouracil at a defined position. We find that, when repair synthesis proceeds through a Pol -dependent single nucleotide replacement mechanism, the 5'-deoxyribosephosphate lyase activity of Pol is essential for repair of both lesions.

AB - The repair of oxidative base lesions in DNA is a coordinated chain of reactions that includes removal of the damaged base, incision of the phosphodiester backbone at the abasic sugar residue, incorporation of an undamaged nucleotide and sealing of the DNA strand break. Although removal of a damaged base in mammalian cells is initiated primarily by a damage-specific DNA glycosylase, several lyases and DNA polymerases may contribute to the later stages of repair. DNA polymerase (Pol ) was implicated recently as the major polymerase involved in repair of oxidative base lesions; however, the identity of the lyase participating in the repair of oxidative lesions is unclear. We studied the mechanism by which mammalian cell extracts process DNA substrates containing a single 8-oxoguanine or 5,6-dihydrouracil at a defined position. We find that, when repair synthesis proceeds through a Pol -dependent single nucleotide replacement mechanism, the 5'-deoxyribosephosphate lyase activity of Pol is essential for repair of both lesions.

KW - base excision repair

KW - cell extracts

KW - DNA polymerase

KW - NTH1

KW - OGG1

U2 - 10.1093/emboj/20.23.6919

DO - 10.1093/emboj/20.23.6919

M3 - Journal article

VL - 20

SP - 6919

EP - 6926

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 23

ER -