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DNA sequence variation and methylation in an arsenic tolerant earthworm population

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DNA sequence variation and methylation in an arsenic tolerant earthworm population. / Kille, Peter; Andre, Jane; Anderson, Craig et al.
In: Soil Biology and Biochemistry, Vol. 57, 02.2013, p. 524-532.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Kille, P, Andre, J, Anderson, C, Ang, HN, Bruford, MW, Bundy, JG, Donnelly, R, Hodson, ME, Juma, G, Lahive, E, Morgan, AJ, Sturzenbaum, SR & Spurgeon, DJ 2013, 'DNA sequence variation and methylation in an arsenic tolerant earthworm population', Soil Biology and Biochemistry, vol. 57, pp. 524-532. https://doi.org/10.1016/j.soilbio.2012.10.014

APA

Kille, P., Andre, J., Anderson, C., Ang, H. N., Bruford, M. W., Bundy, J. G., Donnelly, R., Hodson, M. E., Juma, G., Lahive, E., Morgan, A. J., Sturzenbaum, S. R., & Spurgeon, D. J. (2013). DNA sequence variation and methylation in an arsenic tolerant earthworm population. Soil Biology and Biochemistry, 57, 524-532. https://doi.org/10.1016/j.soilbio.2012.10.014

Vancouver

Kille P, Andre J, Anderson C, Ang HN, Bruford MW, Bundy JG et al. DNA sequence variation and methylation in an arsenic tolerant earthworm population. Soil Biology and Biochemistry. 2013 Feb;57:524-532. Epub 2012 Oct 27. doi: 10.1016/j.soilbio.2012.10.014

Author

Kille, Peter ; Andre, Jane ; Anderson, Craig et al. / DNA sequence variation and methylation in an arsenic tolerant earthworm population. In: Soil Biology and Biochemistry. 2013 ; Vol. 57. pp. 524-532.

Bibtex

@article{0f57431f35fe4861ac9c75a01668b26f,
title = "DNA sequence variation and methylation in an arsenic tolerant earthworm population",
abstract = "Evidence is emerging that earthworms can evolve tolerance to trace element enriched soils. However, few studies have sought to establish whether such tolerance is determined through adaptation or plasticity. Here we report results from a combined analysis of mitochondrial (cytochrome oxidase II, COII), nuclear (amplified fragment length polymorphism, AFLP) variation and DNA methylation in populations of the earthworm Lumbricus rubellus from sites across an abandoned arsenic and copper mine. Earthworms from the mine site population demonstrated clear arsenic tolerance in comparison to a na{\"i}ve strain. COII and AFLP results suggest that L. rubellus from the unexposed and the adapted populations comprises two cryptic lineages (Lineages A and B) each of which was present across all of the sites. AFLP analysis by lineage highlighted variations associated with soil metal/metalloid concentrations (most clearly for Lineage A) suggesting a genetic component to the observed tolerance. The methylation sensitive AFLP (Me-AFLP) identified a high genome methylation content (average 13.5%) in both lineages. For Lineage A, Me-AFLP analysis did not identify a strong association with soil arsenic levels. For Lineage B, however, a clear association of methylation patterns with soil arsenic concentrations was found. This suggests that Lineage B earthworms utilise epigenetic mechanisms to adapt to the presence of contamination. These fundamentally different genetic adjustments in the two clades indicate that the two lineages employ distinct adaptive strategies (genetic or epigenetic) in response to arsenic exposure. Mechanisms driving this variation may be founded within the colonisation histories of the lineages.",
keywords = "Arsenic tolerance, Cryptic lineages, Adaptive variation, DNA methylation, Epigenetics",
author = "Peter Kille and Jane Andre and Craig Anderson and Ang, {Hui Na} and Bruford, {Michael W.} and Bundy, {Jacob G.} and Robert Donnelly and Hodson, {Mark E.} and Gabriela Juma and Elma Lahive and Morgan, {A. John} and Sturzenbaum, {Stephen R.} and Spurgeon, {David J.}",
year = "2013",
month = feb,
doi = "10.1016/j.soilbio.2012.10.014",
language = "English",
volume = "57",
pages = "524--532",
journal = "Soil Biology and Biochemistry",
issn = "0038-0717",
publisher = "Elsevier Ltd",

}

RIS

TY - JOUR

T1 - DNA sequence variation and methylation in an arsenic tolerant earthworm population

AU - Kille, Peter

AU - Andre, Jane

AU - Anderson, Craig

AU - Ang, Hui Na

AU - Bruford, Michael W.

AU - Bundy, Jacob G.

AU - Donnelly, Robert

AU - Hodson, Mark E.

AU - Juma, Gabriela

AU - Lahive, Elma

AU - Morgan, A. John

AU - Sturzenbaum, Stephen R.

AU - Spurgeon, David J.

PY - 2013/2

Y1 - 2013/2

N2 - Evidence is emerging that earthworms can evolve tolerance to trace element enriched soils. However, few studies have sought to establish whether such tolerance is determined through adaptation or plasticity. Here we report results from a combined analysis of mitochondrial (cytochrome oxidase II, COII), nuclear (amplified fragment length polymorphism, AFLP) variation and DNA methylation in populations of the earthworm Lumbricus rubellus from sites across an abandoned arsenic and copper mine. Earthworms from the mine site population demonstrated clear arsenic tolerance in comparison to a naïve strain. COII and AFLP results suggest that L. rubellus from the unexposed and the adapted populations comprises two cryptic lineages (Lineages A and B) each of which was present across all of the sites. AFLP analysis by lineage highlighted variations associated with soil metal/metalloid concentrations (most clearly for Lineage A) suggesting a genetic component to the observed tolerance. The methylation sensitive AFLP (Me-AFLP) identified a high genome methylation content (average 13.5%) in both lineages. For Lineage A, Me-AFLP analysis did not identify a strong association with soil arsenic levels. For Lineage B, however, a clear association of methylation patterns with soil arsenic concentrations was found. This suggests that Lineage B earthworms utilise epigenetic mechanisms to adapt to the presence of contamination. These fundamentally different genetic adjustments in the two clades indicate that the two lineages employ distinct adaptive strategies (genetic or epigenetic) in response to arsenic exposure. Mechanisms driving this variation may be founded within the colonisation histories of the lineages.

AB - Evidence is emerging that earthworms can evolve tolerance to trace element enriched soils. However, few studies have sought to establish whether such tolerance is determined through adaptation or plasticity. Here we report results from a combined analysis of mitochondrial (cytochrome oxidase II, COII), nuclear (amplified fragment length polymorphism, AFLP) variation and DNA methylation in populations of the earthworm Lumbricus rubellus from sites across an abandoned arsenic and copper mine. Earthworms from the mine site population demonstrated clear arsenic tolerance in comparison to a naïve strain. COII and AFLP results suggest that L. rubellus from the unexposed and the adapted populations comprises two cryptic lineages (Lineages A and B) each of which was present across all of the sites. AFLP analysis by lineage highlighted variations associated with soil metal/metalloid concentrations (most clearly for Lineage A) suggesting a genetic component to the observed tolerance. The methylation sensitive AFLP (Me-AFLP) identified a high genome methylation content (average 13.5%) in both lineages. For Lineage A, Me-AFLP analysis did not identify a strong association with soil arsenic levels. For Lineage B, however, a clear association of methylation patterns with soil arsenic concentrations was found. This suggests that Lineage B earthworms utilise epigenetic mechanisms to adapt to the presence of contamination. These fundamentally different genetic adjustments in the two clades indicate that the two lineages employ distinct adaptive strategies (genetic or epigenetic) in response to arsenic exposure. Mechanisms driving this variation may be founded within the colonisation histories of the lineages.

KW - Arsenic tolerance

KW - Cryptic lineages

KW - Adaptive variation

KW - DNA methylation

KW - Epigenetics

U2 - 10.1016/j.soilbio.2012.10.014

DO - 10.1016/j.soilbio.2012.10.014

M3 - Journal article

VL - 57

SP - 524

EP - 532

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

ER -