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DNA Strand Break Rejoining Defect in xrs-6 Is Complemented by Transfection with the Human Ku80 Gene.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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DNA Strand Break Rejoining Defect in xrs-6 Is Complemented by Transfection with the Human Ku80 Gene. / Ross, Gillian; Eady, John J.; Mithal, Natasha P. et al.
In: Cancer Research, Vol. 55, No. 6, 15.03.1995, p. 1235-1238.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Ross, G, Eady, JJ, Mithal, NP, Bush, C, Steel, GG, Jeggo, PA & McMillan, TJ 1995, 'DNA Strand Break Rejoining Defect in xrs-6 Is Complemented by Transfection with the Human Ku80 Gene.', Cancer Research, vol. 55, no. 6, pp. 1235-1238. <http://cancerres.aacrjournals.org/content/55/6/1235.abstract>

APA

Ross, G., Eady, J. J., Mithal, N. P., Bush, C., Steel, G. G., Jeggo, P. A., & McMillan, T. J. (1995). DNA Strand Break Rejoining Defect in xrs-6 Is Complemented by Transfection with the Human Ku80 Gene. Cancer Research, 55(6), 1235-1238. http://cancerres.aacrjournals.org/content/55/6/1235.abstract

Vancouver

Ross G, Eady JJ, Mithal NP, Bush C, Steel GG, Jeggo PA et al. DNA Strand Break Rejoining Defect in xrs-6 Is Complemented by Transfection with the Human Ku80 Gene. Cancer Research. 1995 Mar 15;55(6):1235-1238.

Author

Ross, Gillian ; Eady, John J. ; Mithal, Natasha P. et al. / DNA Strand Break Rejoining Defect in xrs-6 Is Complemented by Transfection with the Human Ku80 Gene. In: Cancer Research. 1995 ; Vol. 55, No. 6. pp. 1235-1238.

Bibtex

@article{b64b434b335e4540b4abf8a28d04bf71,
title = "DNA Strand Break Rejoining Defect in xrs-6 Is Complemented by Transfection with the Human Ku80 Gene.",
abstract = "The radiosensitive mutant xrs-6, derived from Chinese hamster ovary cell line CHO-K1, has been demonstrated to be defective in DNA double-strand break repair and also in its proficiency to undergo V(D)J recombination. Recent work has provided both genetic and biochemical evidence that the Mr 80,000 subunit of the Ku protein is able to complement the radiosensitivity and the V(D)J recombination defect in the xrs-6 mutant. We demonstrate here that complementation of the radiosensitive phenotype in xrs-6 cells by the introduction of Ku80 cDNA is accompanied by the concomitant restoration of DNA double-strand break rejoining proficiency to almost that of the parental CHO-K1 cells, as measured both by neutral single-cell microgel electrophoresis (Comet) technique and by pulsed-field gel electrophoresis. These results provide further biochemical evidence for the involvement of the Ku protein in the repair of DNA double-strand breaks.",
author = "Gillian Ross and Eady, {John J.} and Mithal, {Natasha P.} and Cyd Bush and Steel, {G. Gordon} and Jeggo, {Penny A.} and McMillan, {Trevor J.}",
year = "1995",
month = mar,
day = "15",
language = "English",
volume = "55",
pages = "1235--1238",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "6",

}

RIS

TY - JOUR

T1 - DNA Strand Break Rejoining Defect in xrs-6 Is Complemented by Transfection with the Human Ku80 Gene.

AU - Ross, Gillian

AU - Eady, John J.

AU - Mithal, Natasha P.

AU - Bush, Cyd

AU - Steel, G. Gordon

AU - Jeggo, Penny A.

AU - McMillan, Trevor J.

PY - 1995/3/15

Y1 - 1995/3/15

N2 - The radiosensitive mutant xrs-6, derived from Chinese hamster ovary cell line CHO-K1, has been demonstrated to be defective in DNA double-strand break repair and also in its proficiency to undergo V(D)J recombination. Recent work has provided both genetic and biochemical evidence that the Mr 80,000 subunit of the Ku protein is able to complement the radiosensitivity and the V(D)J recombination defect in the xrs-6 mutant. We demonstrate here that complementation of the radiosensitive phenotype in xrs-6 cells by the introduction of Ku80 cDNA is accompanied by the concomitant restoration of DNA double-strand break rejoining proficiency to almost that of the parental CHO-K1 cells, as measured both by neutral single-cell microgel electrophoresis (Comet) technique and by pulsed-field gel electrophoresis. These results provide further biochemical evidence for the involvement of the Ku protein in the repair of DNA double-strand breaks.

AB - The radiosensitive mutant xrs-6, derived from Chinese hamster ovary cell line CHO-K1, has been demonstrated to be defective in DNA double-strand break repair and also in its proficiency to undergo V(D)J recombination. Recent work has provided both genetic and biochemical evidence that the Mr 80,000 subunit of the Ku protein is able to complement the radiosensitivity and the V(D)J recombination defect in the xrs-6 mutant. We demonstrate here that complementation of the radiosensitive phenotype in xrs-6 cells by the introduction of Ku80 cDNA is accompanied by the concomitant restoration of DNA double-strand break rejoining proficiency to almost that of the parental CHO-K1 cells, as measured both by neutral single-cell microgel electrophoresis (Comet) technique and by pulsed-field gel electrophoresis. These results provide further biochemical evidence for the involvement of the Ku protein in the repair of DNA double-strand breaks.

M3 - Journal article

VL - 55

SP - 1235

EP - 1238

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 6

ER -