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Does the combination of citrate and phytase exudation in Nicotiana tabacum promote the acquisition of endogenous soil organic phosphorus?

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Does the combination of citrate and phytase exudation in Nicotiana tabacum promote the acquisition of endogenous soil organic phosphorus? / Giles, Courtney D.; George, Timothy S.; Brown, Lawrie et al.
In: Plant and Soil, Vol. 412, No. 1-2, 03.2017, p. 43-59.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Giles, CD, George, TS, Brown, L, Mezeli, M, Richardson, AE, Shand, C, Wendler, R, Darch, T, Blackburn, DM, Cooper, P, Stutter, M, Lumsdon, D, Blackwell, MSA, Wearing, CL, Zhang, H & Haygarth, PM 2017, 'Does the combination of citrate and phytase exudation in Nicotiana tabacum promote the acquisition of endogenous soil organic phosphorus?', Plant and Soil, vol. 412, no. 1-2, pp. 43-59. https://doi.org/10.1007/s11104-016-2884-3

APA

Giles, C. D., George, T. S., Brown, L., Mezeli, M., Richardson, A. E., Shand, C., Wendler, R., Darch, T., Blackburn, D. M., Cooper, P., Stutter, M., Lumsdon, D., Blackwell, M. S. A., Wearing, C. L., Zhang, H., & Haygarth, P. M. (2017). Does the combination of citrate and phytase exudation in Nicotiana tabacum promote the acquisition of endogenous soil organic phosphorus? Plant and Soil, 412(1-2), 43-59. https://doi.org/10.1007/s11104-016-2884-3

Vancouver

Giles CD, George TS, Brown L, Mezeli M, Richardson AE, Shand C et al. Does the combination of citrate and phytase exudation in Nicotiana tabacum promote the acquisition of endogenous soil organic phosphorus? Plant and Soil. 2017 Mar;412(1-2):43-59. Epub 2016 Jul 11. doi: 10.1007/s11104-016-2884-3

Author

Giles, Courtney D. ; George, Timothy S. ; Brown, Lawrie et al. / Does the combination of citrate and phytase exudation in Nicotiana tabacum promote the acquisition of endogenous soil organic phosphorus?. In: Plant and Soil. 2017 ; Vol. 412, No. 1-2. pp. 43-59.

Bibtex

@article{bb43d3f00efc486596453e8a7a7a8a9d,
title = "Does the combination of citrate and phytase exudation in Nicotiana tabacum promote the acquisition of endogenous soil organic phosphorus?",
abstract = "Background and Aims Plant acquisition of endogenous forms of soil phosphorus (P) could reduce external P requirements in agricultural systems. This study investigated the interaction of citrate and phytase exudation in controlling the accumulation of P and depletion of soil organic P by transgenic Nicotiana tabacum plants. Methods N. tabacum plant lines including wild-type, vector controls, transgenic plants with single-trait expression of a citrate transporter (A. thaliana frd3) or fungal phytases (phyA: A. niger, P. lycii) and crossed plant lines expressing both traits, were characterized for citrate efflux and phytase exudation. Monocultures and intercropped combinations of single-trait plants were grown in a low available P soil (12 weeks). Plant biomass, shoot P accumulation, rhizosphere soil pH and citrate-extractable-P fractions were determined. Land Equivalent Ratio and complementarity effect was determined in intercropped treatments and multiple-linear-regression was used to predict shoot P accumulation based on plant exudation and soil P depletion. Results Crossed plant lines with co-expression of citrate and phytase accumulated more shoot P than single-trait and intercropped plant treatments. Shoot P accumulation was predicted based on phytase-labile soil P, citrate efflux, and phytase activity (Rsq=0.58, P < .0001). Positive complementarity occurred between intercropped citrate- and phytase-exuding plants, with the greatest gains in shoot P occurring in plant treatments with A. niger phyA expression. Conclusions We show for the first time that trait synergism associated with the exudation of citrate and phytase by tobacco can be linked to the improved acquisition of P and the depletion of soil organic P.",
keywords = "Complementarity , Root exudation , Rhizosphere, Citrate , Phytase , Soil organic phosphorus ",
author = "Giles, {Courtney D.} and George, {Timothy S.} and Lawrie Brown and Malika Mezeli and Richardson, {Alan E.} and Charles Shand and Renate Wendler and Tegan Darch and Blackburn, {Daniel Menezes} and Patricia Cooper and Marc Stutter and David Lumsdon and Blackwell, {Martin S. A.} and Wearing, {Catherine Louise} and Hao Zhang and Haygarth, {Philip Matthew}",
year = "2017",
month = mar,
doi = "10.1007/s11104-016-2884-3",
language = "English",
volume = "412",
pages = "43--59",
journal = "Plant and Soil",
issn = "0032-079X",
publisher = "Springer International Publishing AG",
number = "1-2",

}

RIS

TY - JOUR

T1 - Does the combination of citrate and phytase exudation in Nicotiana tabacum promote the acquisition of endogenous soil organic phosphorus?

AU - Giles, Courtney D.

AU - George, Timothy S.

AU - Brown, Lawrie

AU - Mezeli, Malika

AU - Richardson, Alan E.

AU - Shand, Charles

AU - Wendler, Renate

AU - Darch, Tegan

AU - Blackburn, Daniel Menezes

AU - Cooper, Patricia

AU - Stutter, Marc

AU - Lumsdon, David

AU - Blackwell, Martin S. A.

AU - Wearing, Catherine Louise

AU - Zhang, Hao

AU - Haygarth, Philip Matthew

PY - 2017/3

Y1 - 2017/3

N2 - Background and Aims Plant acquisition of endogenous forms of soil phosphorus (P) could reduce external P requirements in agricultural systems. This study investigated the interaction of citrate and phytase exudation in controlling the accumulation of P and depletion of soil organic P by transgenic Nicotiana tabacum plants. Methods N. tabacum plant lines including wild-type, vector controls, transgenic plants with single-trait expression of a citrate transporter (A. thaliana frd3) or fungal phytases (phyA: A. niger, P. lycii) and crossed plant lines expressing both traits, were characterized for citrate efflux and phytase exudation. Monocultures and intercropped combinations of single-trait plants were grown in a low available P soil (12 weeks). Plant biomass, shoot P accumulation, rhizosphere soil pH and citrate-extractable-P fractions were determined. Land Equivalent Ratio and complementarity effect was determined in intercropped treatments and multiple-linear-regression was used to predict shoot P accumulation based on plant exudation and soil P depletion. Results Crossed plant lines with co-expression of citrate and phytase accumulated more shoot P than single-trait and intercropped plant treatments. Shoot P accumulation was predicted based on phytase-labile soil P, citrate efflux, and phytase activity (Rsq=0.58, P < .0001). Positive complementarity occurred between intercropped citrate- and phytase-exuding plants, with the greatest gains in shoot P occurring in plant treatments with A. niger phyA expression. Conclusions We show for the first time that trait synergism associated with the exudation of citrate and phytase by tobacco can be linked to the improved acquisition of P and the depletion of soil organic P.

AB - Background and Aims Plant acquisition of endogenous forms of soil phosphorus (P) could reduce external P requirements in agricultural systems. This study investigated the interaction of citrate and phytase exudation in controlling the accumulation of P and depletion of soil organic P by transgenic Nicotiana tabacum plants. Methods N. tabacum plant lines including wild-type, vector controls, transgenic plants with single-trait expression of a citrate transporter (A. thaliana frd3) or fungal phytases (phyA: A. niger, P. lycii) and crossed plant lines expressing both traits, were characterized for citrate efflux and phytase exudation. Monocultures and intercropped combinations of single-trait plants were grown in a low available P soil (12 weeks). Plant biomass, shoot P accumulation, rhizosphere soil pH and citrate-extractable-P fractions were determined. Land Equivalent Ratio and complementarity effect was determined in intercropped treatments and multiple-linear-regression was used to predict shoot P accumulation based on plant exudation and soil P depletion. Results Crossed plant lines with co-expression of citrate and phytase accumulated more shoot P than single-trait and intercropped plant treatments. Shoot P accumulation was predicted based on phytase-labile soil P, citrate efflux, and phytase activity (Rsq=0.58, P < .0001). Positive complementarity occurred between intercropped citrate- and phytase-exuding plants, with the greatest gains in shoot P occurring in plant treatments with A. niger phyA expression. Conclusions We show for the first time that trait synergism associated with the exudation of citrate and phytase by tobacco can be linked to the improved acquisition of P and the depletion of soil organic P.

KW - Complementarity

KW - Root exudation

KW - Rhizosphere

KW - Citrate

KW - Phytase

KW - Soil organic phosphorus

U2 - 10.1007/s11104-016-2884-3

DO - 10.1007/s11104-016-2884-3

M3 - Journal article

VL - 412

SP - 43

EP - 59

JO - Plant and Soil

JF - Plant and Soil

SN - 0032-079X

IS - 1-2

ER -