Rights statement: This is the author’s version of a work that was accepted for publication in Sensors and Actuators B: Chemical. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Sensors and Actuators B: Chemical, 330, 2021 DOI: 10.1016/j.snb.2020.129351
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Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Dopamine/mucin-1 functionalized electro-active carbon nanotubes as a probe for direct competitive electrochemical immunosensing of breast cancer biomarker
AU - Rashid, S.
AU - Nawaz, M.H.
AU - Rehman, I.U.
AU - Hayat, A.
AU - Marty, J.L.
N1 - This is the author’s version of a work that was accepted for publication in Sensors and Actuators B: Chemical. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Sensors and Actuators B: Chemical, 330, 2021 DOI: 10.1016/j.snb.2020.129351
PY - 2021/3/1
Y1 - 2021/3/1
N2 - Mucin-1 (MUC-1) is associated with a broad range of human epithelia including gastric, lung and colorectal. In this work, a direct competitive electrochemical immunosensor based on gelatin modified transduction platform was designed. Dopamine (DA)/mucin-1 functionalized electro-active carbon nanotubes were employed as signal generating probes in the construction of electrochemical immunosensor for early stage diagnosis of breast cancer. The gelatin modified electrode served as a support to immobilize antibody (anti-MUC-1), while electrochemical response of functionalized electro-active carbon nano probes was used for quantitative measurement of MUC-1. Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) were carried out to characterize the transduction surface at different fabrication steps. The developed immunosensor permitted the detection of MUC-1 in the linear range of 0.05−940 U/mL, with a detection limit (LOD) of 0.01 U/mL. The immunosensor showed recovery values in the range of 96% for human serum sample analysis, demonstrating its practical applicability.
AB - Mucin-1 (MUC-1) is associated with a broad range of human epithelia including gastric, lung and colorectal. In this work, a direct competitive electrochemical immunosensor based on gelatin modified transduction platform was designed. Dopamine (DA)/mucin-1 functionalized electro-active carbon nanotubes were employed as signal generating probes in the construction of electrochemical immunosensor for early stage diagnosis of breast cancer. The gelatin modified electrode served as a support to immobilize antibody (anti-MUC-1), while electrochemical response of functionalized electro-active carbon nano probes was used for quantitative measurement of MUC-1. Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) were carried out to characterize the transduction surface at different fabrication steps. The developed immunosensor permitted the detection of MUC-1 in the linear range of 0.05−940 U/mL, with a detection limit (LOD) of 0.01 U/mL. The immunosensor showed recovery values in the range of 96% for human serum sample analysis, demonstrating its practical applicability.
KW - Competitive assay
KW - Direct immobilization
KW - Dopamine
KW - Electrochemical immunosensor
KW - Gelatin
KW - Mucin
KW - MWCNTs
U2 - 10.1016/j.snb.2020.129351
DO - 10.1016/j.snb.2020.129351
M3 - Journal article
VL - 330
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
SN - 0925-4005
M1 - 129351
ER -