Final published version
Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Entry for encyclopedia/dictionary
Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Entry for encyclopedia/dictionary
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TY - CHAP
T1 - Droplets- and digital-based methods
AU - Fielden, P.R.
AU - Baldock, S.J.
PY - 2019/2/15
Y1 - 2019/2/15
N2 - Liquid droplets may be manipulated within microfluidic channel structures, or on planar surfaces to provide experimental platforms where each droplet is a discrete experimental entity. Sometimes described as digital methodology, this provides a unique approach within the fields of chemistry and biochemistry. Subsequent splitting, combining and sorting processes with the droplets offer a limitless opportunity for complex experimental design that includes high sample numbers with high-throughput capability. Distinct advantages include nanolitre, or lower sample volumes, rapid mixing and short diffusion distances. When placed within an automated microfluidic environment, droplets may be driven sequentially or in parallel through a pre-set pattern of unit operations. Many applications have been reported that include the broad fields of synthesis and sample assay. Synthetic applications have been reported for organic synthesis, biochemical synthesis and materials synthesis. The majority of assay-based applications have been reported for bio-assays and biological assays with single cells. The distinct advantages of low sample volumes and high-throughput have been the primary drivers for the development of droplet and digital-based methods.
AB - Liquid droplets may be manipulated within microfluidic channel structures, or on planar surfaces to provide experimental platforms where each droplet is a discrete experimental entity. Sometimes described as digital methodology, this provides a unique approach within the fields of chemistry and biochemistry. Subsequent splitting, combining and sorting processes with the droplets offer a limitless opportunity for complex experimental design that includes high sample numbers with high-throughput capability. Distinct advantages include nanolitre, or lower sample volumes, rapid mixing and short diffusion distances. When placed within an automated microfluidic environment, droplets may be driven sequentially or in parallel through a pre-set pattern of unit operations. Many applications have been reported that include the broad fields of synthesis and sample assay. Synthetic applications have been reported for organic synthesis, biochemical synthesis and materials synthesis. The majority of assay-based applications have been reported for bio-assays and biological assays with single cells. The distinct advantages of low sample volumes and high-throughput have been the primary drivers for the development of droplet and digital-based methods.
KW - Applications
KW - Assay
KW - Biochemistry
KW - Chemistry
KW - Detection
KW - Diagnostics
KW - Digital microfluidics
KW - Droplet
KW - High-throughput
KW - Lab-on-a-chip
KW - Microchannel
KW - Synthesis
U2 - 10.1016/B978-0-12-409547-2.13943-5
DO - 10.1016/B978-0-12-409547-2.13943-5
M3 - Entry for encyclopedia/dictionary
SN - 9780081019832
SP - 225
EP - 231
BT - Encyclopedia of Analytical Science
A2 - Worsfold, Paul
A2 - Townshend, Alan
A2 - Poole, Colin
A2 - Miro, Manuel
PB - Elsevier
ER -