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Dynamic regulation of the Trypanosoma brucei transferrin receptor in response to iron starvation is mediated via the 3’UTR

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Dynamic regulation of the Trypanosoma brucei transferrin receptor in response to iron starvation is mediated via the 3’UTR. / Benz, Corinna; Lo, Winston; Fathallah, Nadin; Connor-Guscott, Ashley; Benns, Henry J.; Urbaniak, Michael Daniel.

In: PLoS ONE, Vol. 13, No. 12, e0206332, 31.12.2018.

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Benz, Corinna ; Lo, Winston ; Fathallah, Nadin ; Connor-Guscott, Ashley ; Benns, Henry J. ; Urbaniak, Michael Daniel. / Dynamic regulation of the Trypanosoma brucei transferrin receptor in response to iron starvation is mediated via the 3’UTR. In: PLoS ONE. 2018 ; Vol. 13, No. 12.

Bibtex

@article{dd5bc86e0752434cba080a65cdf621d4,
title = "Dynamic regulation of the Trypanosoma brucei transferrin receptor in response to iron starvation is mediated via the 3{\textquoteright}UTR",
abstract = "The bloodstream form of the parasite Trypanosoma brucei obtains iron from its mammalian host by receptor-mediated endocytosis of host transferrin through its own unique transferrin receptor (TbTfR). Expression of TbTfR rapidly increases upon iron starvation by post-transcriptional regulation through a currently undefined mechanism that is distinct from the mammalian iron response system. We have created reporter cell lines by fusing the TbTfR 3{\textquoteright}UTR or a control Aldolase 3{\textquoteright}UTR to reporter genes encoding GFP or firefly Luciferase, and inserted the fusions into a bloodstream form cell line at a tagged ribosomal RNA locus. Fusion of the TbTfR 3{\textquoteright}UTR is sufficient to significantly repress the expression of the reporter proteins under normal growth conditions. Under iron starvation conditions we observed upregulation of the mRNA and protein level of the TbTfR 3{\textquoteright}UTR fusions only, with a magnitude and timing consistent with that reported for upregulation of the TbTfR. We conclude that the dynamic regulation of the T. brucei transferrin receptor in response to iron starvation is mediated via its 3{\textquoteright}UTR, and that the effect is independent of genomic location.",
author = "Corinna Benz and Winston Lo and Nadin Fathallah and Ashley Connor-Guscott and Benns, {Henry J.} and Urbaniak, {Michael Daniel}",
year = "2018",
month = dec,
day = "31",
doi = "10.1371/journal.pone.0206332",
language = "English",
volume = "13",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",

}

RIS

TY - JOUR

T1 - Dynamic regulation of the Trypanosoma brucei transferrin receptor in response to iron starvation is mediated via the 3’UTR

AU - Benz, Corinna

AU - Lo, Winston

AU - Fathallah, Nadin

AU - Connor-Guscott, Ashley

AU - Benns, Henry J.

AU - Urbaniak, Michael Daniel

PY - 2018/12/31

Y1 - 2018/12/31

N2 - The bloodstream form of the parasite Trypanosoma brucei obtains iron from its mammalian host by receptor-mediated endocytosis of host transferrin through its own unique transferrin receptor (TbTfR). Expression of TbTfR rapidly increases upon iron starvation by post-transcriptional regulation through a currently undefined mechanism that is distinct from the mammalian iron response system. We have created reporter cell lines by fusing the TbTfR 3’UTR or a control Aldolase 3’UTR to reporter genes encoding GFP or firefly Luciferase, and inserted the fusions into a bloodstream form cell line at a tagged ribosomal RNA locus. Fusion of the TbTfR 3’UTR is sufficient to significantly repress the expression of the reporter proteins under normal growth conditions. Under iron starvation conditions we observed upregulation of the mRNA and protein level of the TbTfR 3’UTR fusions only, with a magnitude and timing consistent with that reported for upregulation of the TbTfR. We conclude that the dynamic regulation of the T. brucei transferrin receptor in response to iron starvation is mediated via its 3’UTR, and that the effect is independent of genomic location.

AB - The bloodstream form of the parasite Trypanosoma brucei obtains iron from its mammalian host by receptor-mediated endocytosis of host transferrin through its own unique transferrin receptor (TbTfR). Expression of TbTfR rapidly increases upon iron starvation by post-transcriptional regulation through a currently undefined mechanism that is distinct from the mammalian iron response system. We have created reporter cell lines by fusing the TbTfR 3’UTR or a control Aldolase 3’UTR to reporter genes encoding GFP or firefly Luciferase, and inserted the fusions into a bloodstream form cell line at a tagged ribosomal RNA locus. Fusion of the TbTfR 3’UTR is sufficient to significantly repress the expression of the reporter proteins under normal growth conditions. Under iron starvation conditions we observed upregulation of the mRNA and protein level of the TbTfR 3’UTR fusions only, with a magnitude and timing consistent with that reported for upregulation of the TbTfR. We conclude that the dynamic regulation of the T. brucei transferrin receptor in response to iron starvation is mediated via its 3’UTR, and that the effect is independent of genomic location.

U2 - 10.1371/journal.pone.0206332

DO - 10.1371/journal.pone.0206332

M3 - Journal article

VL - 13

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 12

M1 - e0206332

ER -