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Effect of mutations of residue 340 in the large subunit polypeptide of Rubisco from Anacystis nidulans

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Effect of mutations of residue 340 in the large subunit polypeptide of Rubisco from Anacystis nidulans. / Madgwick, Pippa J.; Parmar, Saroj; Parry, Martin A J.
In: European Journal of Biochemistry, Vol. 253, No. 2, 15.04.1998, p. 476-479.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Madgwick, PJ, Parmar, S & Parry, MAJ 1998, 'Effect of mutations of residue 340 in the large subunit polypeptide of Rubisco from Anacystis nidulans', European Journal of Biochemistry, vol. 253, no. 2, pp. 476-479. https://doi.org/10.1046/j.1432-1327.1998.2530476.x

APA

Madgwick, P. J., Parmar, S., & Parry, M. A. J. (1998). Effect of mutations of residue 340 in the large subunit polypeptide of Rubisco from Anacystis nidulans. European Journal of Biochemistry, 253(2), 476-479. https://doi.org/10.1046/j.1432-1327.1998.2530476.x

Vancouver

Madgwick PJ, Parmar S, Parry MAJ. Effect of mutations of residue 340 in the large subunit polypeptide of Rubisco from Anacystis nidulans. European Journal of Biochemistry. 1998 Apr 15;253(2):476-479. doi: 10.1046/j.1432-1327.1998.2530476.x

Author

Madgwick, Pippa J. ; Parmar, Saroj ; Parry, Martin A J. / Effect of mutations of residue 340 in the large subunit polypeptide of Rubisco from Anacystis nidulans. In: European Journal of Biochemistry. 1998 ; Vol. 253, No. 2. pp. 476-479.

Bibtex

@article{b5fdbefc2385470692d19aff4301b456,
title = "Effect of mutations of residue 340 in the large subunit polypeptide of Rubisco from Anacystis nidulans",
abstract = "Residues 338-342 at the C-terminal end of loop 6 in the large subunit β/α barrel structure of Rubisoo influence specificity towards CO2 and O2. In Anacystis nidulans Rubisco, replacement of alanine 340 by tyrosine or histidine increased the specificity factor by 12-13%, accompanied by a 25- 33% fall in V(c), the rate of carboxylation, while replacement by asparagine increased the specificity factor by 9% and V(c) by 19%. Other mutations did not significantly alter specificity. Alanine 340 does not interact directly with the bisphosphate substrate, thus replacing it with other residues must have indirect effects on the specificity factor and rate of carboxylation.",
keywords = "Large subunit, Loop 6, Ribulose 1,5-bisphosphate, Rubisco, Specificity factor",
author = "Madgwick, {Pippa J.} and Saroj Parmar and Parry, {Martin A J}",
year = "1998",
month = apr,
day = "15",
doi = "10.1046/j.1432-1327.1998.2530476.x",
language = "English",
volume = "253",
pages = "476--479",
journal = "European Journal of Biochemistry",
issn = "0014-2956",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Effect of mutations of residue 340 in the large subunit polypeptide of Rubisco from Anacystis nidulans

AU - Madgwick, Pippa J.

AU - Parmar, Saroj

AU - Parry, Martin A J

PY - 1998/4/15

Y1 - 1998/4/15

N2 - Residues 338-342 at the C-terminal end of loop 6 in the large subunit β/α barrel structure of Rubisoo influence specificity towards CO2 and O2. In Anacystis nidulans Rubisco, replacement of alanine 340 by tyrosine or histidine increased the specificity factor by 12-13%, accompanied by a 25- 33% fall in V(c), the rate of carboxylation, while replacement by asparagine increased the specificity factor by 9% and V(c) by 19%. Other mutations did not significantly alter specificity. Alanine 340 does not interact directly with the bisphosphate substrate, thus replacing it with other residues must have indirect effects on the specificity factor and rate of carboxylation.

AB - Residues 338-342 at the C-terminal end of loop 6 in the large subunit β/α barrel structure of Rubisoo influence specificity towards CO2 and O2. In Anacystis nidulans Rubisco, replacement of alanine 340 by tyrosine or histidine increased the specificity factor by 12-13%, accompanied by a 25- 33% fall in V(c), the rate of carboxylation, while replacement by asparagine increased the specificity factor by 9% and V(c) by 19%. Other mutations did not significantly alter specificity. Alanine 340 does not interact directly with the bisphosphate substrate, thus replacing it with other residues must have indirect effects on the specificity factor and rate of carboxylation.

KW - Large subunit

KW - Loop 6

KW - Ribulose 1,5-bisphosphate

KW - Rubisco

KW - Specificity factor

UR - http://www.scopus.com/inward/record.url?scp=0032522562&partnerID=8YFLogxK

U2 - 10.1046/j.1432-1327.1998.2530476.x

DO - 10.1046/j.1432-1327.1998.2530476.x

M3 - Journal article

C2 - 9654099

AN - SCOPUS:0032522562

VL - 253

SP - 476

EP - 479

JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

SN - 0014-2956

IS - 2

ER -