A hydroponic trial was carried out to determine the effect of molybdenum (Mo) on utilization and uptake of macro and micronutrients in different rice cultivars. The experiment was conducted using a randomized complete-block design, with a split-plot arrangement of treatments and three replications. Four rates of Mo (0, 0.01, 0.1 and 1 mg L-1) and five cultivars (MR219, HASHEMI, MR232, FAJRE and MR253) provided the main and sub-plots, respectively. The results showed that the enhancement of Mo levels (from 0.01 to 0.1 and 0.1 to 1mg L-1) led to increase of root Mo uptake by 81.7 and 61.6% and shoot Mo uptake by 43 and 87%, respectively. Also, Mo application significantly affected shoot Phosphorus uptake so that highest shoot Phosphorus (P) uptake (0.43% plant-1) was achieved at 1mg Mo L-1 but there was no significant influence on root P uptake. Shoot Iron (Fe) uptake was inversely proportional to increment of Mo rates therefore, the highest rate of shoot Fe (59.05 μg plant-1) was obtained in treatments with least amount of applied Mo. Also, increase in Mo application enhanced manganese (Mn) uptake of shoot but there was no significant effect on Mn root uptake.