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Effects of sieving, drying and rewetting upon soil bacterial community structure and respiration rates

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Effects of sieving, drying and rewetting upon soil bacterial community structure and respiration rates. / Thomson, Bruce C.; Ostle, Nick J.; McNamara, Niall P. et al.
In: Journal of Microbiological Methods, Vol. 83, No. 1, 01.10.2010, p. 69-73.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Thomson, BC, Ostle, NJ, McNamara, NP, Whiteley, AS & Griffiths, RI 2010, 'Effects of sieving, drying and rewetting upon soil bacterial community structure and respiration rates', Journal of Microbiological Methods, vol. 83, no. 1, pp. 69-73. https://doi.org/10.1016/j.mimet.2010.07.021

APA

Thomson, B. C., Ostle, N. J., McNamara, N. P., Whiteley, A. S., & Griffiths, R. I. (2010). Effects of sieving, drying and rewetting upon soil bacterial community structure and respiration rates. Journal of Microbiological Methods, 83(1), 69-73. https://doi.org/10.1016/j.mimet.2010.07.021

Vancouver

Thomson BC, Ostle NJ, McNamara NP, Whiteley AS, Griffiths RI. Effects of sieving, drying and rewetting upon soil bacterial community structure and respiration rates. Journal of Microbiological Methods. 2010 Oct 1;83(1):69-73. doi: 10.1016/j.mimet.2010.07.021

Author

Thomson, Bruce C. ; Ostle, Nick J. ; McNamara, Niall P. et al. / Effects of sieving, drying and rewetting upon soil bacterial community structure and respiration rates. In: Journal of Microbiological Methods. 2010 ; Vol. 83, No. 1. pp. 69-73.

Bibtex

@article{cb290ffc9c7c468ca72ee9d399c2ad93,
title = "Effects of sieving, drying and rewetting upon soil bacterial community structure and respiration rates",
abstract = "Soil microcosm studies often require some form of soil homogenisation, such as sieving, to provide a representative sample. Frequently, soils are also homogenised following drying and are then rewetted, yet little research has been done to understand how these methods impact upon microbial communities. Here we compared the molecular diversity and functional responses of intact cores from a Scottish grassland soil with homogenised samples prepared by drying, sieving and rewetting or freshly sieving wet soils. Results showed that there was no significant difference in total soil CO2-C efflux between the freshly sieved and intact core treatments, however, respiration was significantly higher in the dried and rewetted microcosms. Molecular fingerprinting (T-RFLP) of bacterial communities at two different time-points showed that both homogenisation methods significantly altered bacterial community structure with the largest differences being observed after drying and rewetting. Assessments of responsive taxa in each treatment showed that intact cores were dominated by Acidobacterial peaks whereas an increased relative abundance of Alphaproteobacterial terminal restriction fragments were apparent in both homogenised treatments. However, the shift in community structure was not as large in the freshly sieved soil. Our findings suggest that if soil homogenisation must be performed, then fresh sieving of wet soil is preferable to drying and rewetting in approximating the bacterial diversity and functioning of intact cores.",
keywords = "Bacterial community structure, Drying and rewetting, Respiration, Sieving, Soil, T-RFLP",
author = "Thomson, {Bruce C.} and Ostle, {Nick J.} and McNamara, {Niall P.} and Whiteley, {Andrew S.} and Griffiths, {Robert I.}",
year = "2010",
month = oct,
day = "1",
doi = "10.1016/j.mimet.2010.07.021",
language = "English",
volume = "83",
pages = "69--73",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Effects of sieving, drying and rewetting upon soil bacterial community structure and respiration rates

AU - Thomson, Bruce C.

AU - Ostle, Nick J.

AU - McNamara, Niall P.

AU - Whiteley, Andrew S.

AU - Griffiths, Robert I.

PY - 2010/10/1

Y1 - 2010/10/1

N2 - Soil microcosm studies often require some form of soil homogenisation, such as sieving, to provide a representative sample. Frequently, soils are also homogenised following drying and are then rewetted, yet little research has been done to understand how these methods impact upon microbial communities. Here we compared the molecular diversity and functional responses of intact cores from a Scottish grassland soil with homogenised samples prepared by drying, sieving and rewetting or freshly sieving wet soils. Results showed that there was no significant difference in total soil CO2-C efflux between the freshly sieved and intact core treatments, however, respiration was significantly higher in the dried and rewetted microcosms. Molecular fingerprinting (T-RFLP) of bacterial communities at two different time-points showed that both homogenisation methods significantly altered bacterial community structure with the largest differences being observed after drying and rewetting. Assessments of responsive taxa in each treatment showed that intact cores were dominated by Acidobacterial peaks whereas an increased relative abundance of Alphaproteobacterial terminal restriction fragments were apparent in both homogenised treatments. However, the shift in community structure was not as large in the freshly sieved soil. Our findings suggest that if soil homogenisation must be performed, then fresh sieving of wet soil is preferable to drying and rewetting in approximating the bacterial diversity and functioning of intact cores.

AB - Soil microcosm studies often require some form of soil homogenisation, such as sieving, to provide a representative sample. Frequently, soils are also homogenised following drying and are then rewetted, yet little research has been done to understand how these methods impact upon microbial communities. Here we compared the molecular diversity and functional responses of intact cores from a Scottish grassland soil with homogenised samples prepared by drying, sieving and rewetting or freshly sieving wet soils. Results showed that there was no significant difference in total soil CO2-C efflux between the freshly sieved and intact core treatments, however, respiration was significantly higher in the dried and rewetted microcosms. Molecular fingerprinting (T-RFLP) of bacterial communities at two different time-points showed that both homogenisation methods significantly altered bacterial community structure with the largest differences being observed after drying and rewetting. Assessments of responsive taxa in each treatment showed that intact cores were dominated by Acidobacterial peaks whereas an increased relative abundance of Alphaproteobacterial terminal restriction fragments were apparent in both homogenised treatments. However, the shift in community structure was not as large in the freshly sieved soil. Our findings suggest that if soil homogenisation must be performed, then fresh sieving of wet soil is preferable to drying and rewetting in approximating the bacterial diversity and functioning of intact cores.

KW - Bacterial community structure

KW - Drying and rewetting

KW - Respiration

KW - Sieving

KW - Soil

KW - T-RFLP

U2 - 10.1016/j.mimet.2010.07.021

DO - 10.1016/j.mimet.2010.07.021

M3 - Journal article

C2 - 20691223

AN - SCOPUS:77956411371

VL - 83

SP - 69

EP - 73

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

IS - 1

ER -