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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - EIN2L, EIL3, and EIL4 Modulates Drought Resistance and Growth by Regulating Stomatal Movement in Soybean
AU - Ban, Meihan
AU - Lv, Qiang
AU - Liu, Jianfeng
AU - Dodd, Ian C.
AU - He, Hongli
AU - Lv, Jiaqi
AU - Wang, Fanke
AU - Zhao, Shuang
AU - Zhang, Xinyue
AU - Yu, Hengshu
AU - Wang, Shuai
AU - Zhao, Yang
AU - Cheng, Yunqing
PY - 2025/6/2
Y1 - 2025/6/2
N2 - ABSTRACTSoybean (Glycine max) is a globally important crop for oil, grain and feed; however, drought stress limits its yield and quality. Ethylene alters plant development, stress responses and yield, but the molecular mechanism(s) by which it regulates these processes have not been fully elucidated. Here we show the regulatory factors EIN2L, EIL3, and EIL4 modulate stomatal movement and drought induced‐ABA response. Plants with a triple EIN2L, EIL3, and EIL4 CRISPR/Cas9 knockout showed attenuated darkness, ABA‐ and ethylene‐mediated stomatal closure, with altered expression of genes related to ROS, NO and the anionic channels SLAHs. Furthermore, EIN2L, EIL3, and EIL4 regulators positively regulate ethylene synthesis genes (ACS and ACO) during darkness and ABA synthesis (NCED3) and sensitivity (PYL8) genes following ABA treatment. Higher photosynthesis of well‐watered plants and stomatal opening of the knockout line, along with its diminished sensitivity to ABA, enhanced water use thereby increasing sensitivity to drought. Selecting for allelic variation in EIN2L, EIL3, and EIL4 genes could better adapt soybean cultivars to the prevailing soil water availability, according to whether water conserving (WT alleles) or water‐spending (knockout lines) traits enhance crop yields.
AB - ABSTRACTSoybean (Glycine max) is a globally important crop for oil, grain and feed; however, drought stress limits its yield and quality. Ethylene alters plant development, stress responses and yield, but the molecular mechanism(s) by which it regulates these processes have not been fully elucidated. Here we show the regulatory factors EIN2L, EIL3, and EIL4 modulate stomatal movement and drought induced‐ABA response. Plants with a triple EIN2L, EIL3, and EIL4 CRISPR/Cas9 knockout showed attenuated darkness, ABA‐ and ethylene‐mediated stomatal closure, with altered expression of genes related to ROS, NO and the anionic channels SLAHs. Furthermore, EIN2L, EIL3, and EIL4 regulators positively regulate ethylene synthesis genes (ACS and ACO) during darkness and ABA synthesis (NCED3) and sensitivity (PYL8) genes following ABA treatment. Higher photosynthesis of well‐watered plants and stomatal opening of the knockout line, along with its diminished sensitivity to ABA, enhanced water use thereby increasing sensitivity to drought. Selecting for allelic variation in EIN2L, EIL3, and EIL4 genes could better adapt soybean cultivars to the prevailing soil water availability, according to whether water conserving (WT alleles) or water‐spending (knockout lines) traits enhance crop yields.
U2 - 10.1111/pce.15651
DO - 10.1111/pce.15651
M3 - Journal article
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
SN - 0140-7791
ER -