Final published version, 3.63 MB, PDF document
Available under license: CC BY-NC: Creative Commons Attribution-NonCommercial 4.0 International License
Final published version
Licence: CC BY-NC: Creative Commons Attribution-NonCommercial 4.0 International License
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Electrically Conductive and 3D-Printable Oxidized Alginate-Gelatin Polypyrrole
T2 - PSS Hydrogels for Tissue Engineering
AU - Distler, Thomas
AU - Polley, Christian
AU - Shi, Fukun
AU - Schneidereit, Dominik
AU - Ashton, Mark
AU - Kolb, Jürgen
AU - Friedrich, Oliver
AU - Hardy, John
AU - Detsch, Rainer
AU - Seitz, Hermann
AU - Boccaccini, Aldo
PY - 2021/5/5
Y1 - 2021/5/5
N2 - Electroactive hydrogels can be used to influence cell response and maturationby electrical stimulation. However, hydrogel formulations which are 3Dprintable, electroactive, cytocompatible, and allow cell adhesion, remain achallenge in the design of such stimuli-responsive biomaterials for tissueengineering. Here, a combination of pyrrole with a high gelatin-contentoxidized alginate-gelatin (ADA-GEL) hydrogel is reported, offering3D-printability of hydrogel precursors to prepare cytocompatible andelectrically conductive hydrogel scaffolds. By oxidation of pyrrole,electroactive polypyrrole:polystyrenesulfonate (PPy:PSS) is synthesized insidethe ADA-GEL matrix. The hydrogels are assessed regarding theirelectrical/mechanical properties, 3D-printability, and cytocompatibility. It ispossible to prepare open-porous scaffolds via bioplotting which areelectrically conductive and have a higher cell seeding efficiency in scaffolddepth in comparison to flat 2D hydrogels, which is confirmed via multiphotonfluorescence microscopy. The formation of an interpenetrating polypyrrolematrix in the hydrogel matrix increases the conductivity and stiffness of thehydrogels, maintaining the capacity of the gels to promote cell adhesion andproliferation. The results demonstrate that a 3D-printable ADA-GEL can berendered conductive (ADA-GEL-PPy:PSS), and that such hydrogelformulations have promise for cell therapies, in vitro cell culture, andelectrical-stimulation assisted tissue engineering.
AB - Electroactive hydrogels can be used to influence cell response and maturationby electrical stimulation. However, hydrogel formulations which are 3Dprintable, electroactive, cytocompatible, and allow cell adhesion, remain achallenge in the design of such stimuli-responsive biomaterials for tissueengineering. Here, a combination of pyrrole with a high gelatin-contentoxidized alginate-gelatin (ADA-GEL) hydrogel is reported, offering3D-printability of hydrogel precursors to prepare cytocompatible andelectrically conductive hydrogel scaffolds. By oxidation of pyrrole,electroactive polypyrrole:polystyrenesulfonate (PPy:PSS) is synthesized insidethe ADA-GEL matrix. The hydrogels are assessed regarding theirelectrical/mechanical properties, 3D-printability, and cytocompatibility. It ispossible to prepare open-porous scaffolds via bioplotting which areelectrically conductive and have a higher cell seeding efficiency in scaffolddepth in comparison to flat 2D hydrogels, which is confirmed via multiphotonfluorescence microscopy. The formation of an interpenetrating polypyrrolematrix in the hydrogel matrix increases the conductivity and stiffness of thehydrogels, maintaining the capacity of the gels to promote cell adhesion andproliferation. The results demonstrate that a 3D-printable ADA-GEL can berendered conductive (ADA-GEL-PPy:PSS), and that such hydrogelformulations have promise for cell therapies, in vitro cell culture, andelectrical-stimulation assisted tissue engineering.
KW - electroactive
U2 - 10.1002/adhm.202001876
DO - 10.1002/adhm.202001876
M3 - Journal article
VL - 10
JO - Advanced Healthcare Materials
JF - Advanced Healthcare Materials
SN - 2192-2659
IS - 9
M1 - 2001876
ER -