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Electrolyte homeostasis in urothelial neoplasia: the effects of amiloride.

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Electrolyte homeostasis in urothelial neoplasia: the effects of amiloride. / Cartman, M. L.; Morris, J. A.; Huddart, H. et al.
In: British Journal of Urology, Vol. 75, No. 5, 05.1995, p. 599-603.

Research output: Contribution to Journal/Magazine › Journal article

Harvard

Cartman, ML, Morris, JA, Huddart, H & Staff, WG 1995, 'Electrolyte homeostasis in urothelial neoplasia: the effects of amiloride.', British Journal of Urology, vol. 75, no. 5, pp. 599-603. https://doi.org/10.1111/j.1464-410X.1995.tb07416.x

APA

Cartman, M. L., Morris, J. A., Huddart, H., & Staff, W. G. (1995). Electrolyte homeostasis in urothelial neoplasia: the effects of amiloride. British Journal of Urology, 75(5), 599-603. https://doi.org/10.1111/j.1464-410X.1995.tb07416.x

Vancouver

Cartman ML, Morris JA, Huddart H, Staff WG. Electrolyte homeostasis in urothelial neoplasia: the effects of amiloride. British Journal of Urology. 1995 May;75(5):599-603. doi: 10.1111/j.1464-410X.1995.tb07416.x

Author

Cartman, M. L. ; Morris, J. A. ; Huddart, H. et al. / Electrolyte homeostasis in urothelial neoplasia: the effects of amiloride. In: British Journal of Urology. 1995 ; Vol. 75, No. 5. pp. 599-603.

Bibtex

@article{4b09638c3ed54e43a7e75e8e50d27caa,
title = "Electrolyte homeostasis in urothelial neoplasia: the effects of amiloride.",
abstract = "Objective To investigate the effect of amiloride pre-incubation on the intracellular electrolyte levels in neoplastic and non-neoplastic urothelium. Materials and methods Samples of papillary transitional cell carcinomas (grades 1 and 2) and non-neoplastic human urothelium were either frozen immediately or after pre-incubation with Krebs saline alone or with Krebs saline containing amiloride. The frozen samples were planed in a cryo-ultramicrotome and then examined by low temperature scanning electron microscopy. Intracellular electrolyte levels were measured using X-ray microanalysis. Results In the cells frozen immediately the ratios K+/Na+, K+/P and K+/Cl- were significantly increased in neoplastic compared with non-neoplastic urothelial cells (P<0.001). This difference was also found in the cells pre-incubated with Krebs saline alone but was not present in the cells pre-incubated with amiloride. Conclusions These results were consistent with the concept that activation of the amiloride-sensitive Na+/H + membrane exchanger is a primary event in neoplasia.",
keywords = "Amiloride • LTSEM • Na+/H+ antiport • urothelial neoplasia • X-ray microanalysis",
author = "Cartman, {M. L.} and Morris, {J. A.} and H. Huddart and Staff, {W. G.}",
year = "1995",
month = may,
doi = "10.1111/j.1464-410X.1995.tb07416.x",
language = "English",
volume = "75",
pages = "599--603",
journal = "British Journal of Urology",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - Electrolyte homeostasis in urothelial neoplasia: the effects of amiloride.

AU - Cartman, M. L.

AU - Morris, J. A.

AU - Huddart, H.

AU - Staff, W. G.

PY - 1995/5

Y1 - 1995/5

N2 - Objective To investigate the effect of amiloride pre-incubation on the intracellular electrolyte levels in neoplastic and non-neoplastic urothelium. Materials and methods Samples of papillary transitional cell carcinomas (grades 1 and 2) and non-neoplastic human urothelium were either frozen immediately or after pre-incubation with Krebs saline alone or with Krebs saline containing amiloride. The frozen samples were planed in a cryo-ultramicrotome and then examined by low temperature scanning electron microscopy. Intracellular electrolyte levels were measured using X-ray microanalysis. Results In the cells frozen immediately the ratios K+/Na+, K+/P and K+/Cl- were significantly increased in neoplastic compared with non-neoplastic urothelial cells (P<0.001). This difference was also found in the cells pre-incubated with Krebs saline alone but was not present in the cells pre-incubated with amiloride. Conclusions These results were consistent with the concept that activation of the amiloride-sensitive Na+/H + membrane exchanger is a primary event in neoplasia.

AB - Objective To investigate the effect of amiloride pre-incubation on the intracellular electrolyte levels in neoplastic and non-neoplastic urothelium. Materials and methods Samples of papillary transitional cell carcinomas (grades 1 and 2) and non-neoplastic human urothelium were either frozen immediately or after pre-incubation with Krebs saline alone or with Krebs saline containing amiloride. The frozen samples were planed in a cryo-ultramicrotome and then examined by low temperature scanning electron microscopy. Intracellular electrolyte levels were measured using X-ray microanalysis. Results In the cells frozen immediately the ratios K+/Na+, K+/P and K+/Cl- were significantly increased in neoplastic compared with non-neoplastic urothelial cells (P<0.001). This difference was also found in the cells pre-incubated with Krebs saline alone but was not present in the cells pre-incubated with amiloride. Conclusions These results were consistent with the concept that activation of the amiloride-sensitive Na+/H + membrane exchanger is a primary event in neoplasia.

KW - Amiloride • LTSEM • Na+/H+ antiport • urothelial neoplasia • X-ray microanalysis

U2 - 10.1111/j.1464-410X.1995.tb07416.x

DO - 10.1111/j.1464-410X.1995.tb07416.x

M3 - Journal article

VL - 75

SP - 599

EP - 603

JO - British Journal of Urology

JF - British Journal of Urology

IS - 5

ER -