Electrolyte Levels in Neoplastic and Nonneoplastic Human Urothelium After Ouabain Treatment: X-Ray Microanalysis of Bulk Hydrated Samples.

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https://doi.org/10.3109/01913129409023220
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Keywords

low-temperature scanning electron microscopy, ouabain, quantitative X-ray microanalysis, urothelial tumors

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Electrolyte Levels in Neoplastic and Nonneoplastic Human Urothelium After Ouabain Treatment: X-Ray Microanalysis of Bulk Hydrated Samples. / Cartman, Michelle L.; Morris, James A.; Oates, Kenneth et al.
In: Ultrastructural Pathology, Vol. 18, No. 5, 1994, p. 461-466.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{89623130e7aa4a61a934e2f06dae1828,

title = "Electrolyte Levels in Neoplastic and Nonneoplastic Human Urothelium After Ouabain Treatment: X-Ray Microanalysis of Bulk Hydrated Samples.",

abstract = "Samples of neoplastic and nonneoplastic human urothelium were immediately frozen, incubated in Krebs' saline and then frozen, or incubated in 10-5 mol/L ouabain in Krebs' saline and then frozen. The frozen specimens were then planed in a cryoultramicrotome and examined by low-temperature scanning electron microscopy. X-Ray microanalysis was performed on the superficial urothelial cells. Neoplastic cells immediately frozen and those incubated in Krebs' saline had significantly higher K+/Na+, K+/P, and K+/Cl- ratios and lower Na+/P and Cl-/P ratios than nonneoplastic cells. Incubation in ouabain led to a fall in the K+/Na+, K+/P, and K+/Cl- ratios and a rise in the Na+/P and Na+/Cl- ratios in both neoplastic and nonneoplastic cells and effectively nullified the difference between them. These results are consistent with the concept that in neoplasia a primary event is stimulation of Na+/H+ exchange, which leads to secondary stimulation of the ouabain-sensitive Na+/K+ ATPase pump.",

keywords = "low-temperature scanning electron microscopy, ouabain, quantitative X-ray microanalysis, urothelial tumors",

author = "Cartman, {Michelle L.} and Morris, {James A.} and Kenneth Oates and Henry Huddart and Staff, {William G.}",

year = "1994",

doi = "10.3109/01913129409023220",

language = "English",

volume = "18",

pages = "461--466",

journal = "Ultrastructural Pathology",

issn = "1521-0758",

publisher = "Informa Healthcare",

number = "5",

}

RIS

TY - JOUR

T1 - Electrolyte Levels in Neoplastic and Nonneoplastic Human Urothelium After Ouabain Treatment: X-Ray Microanalysis of Bulk Hydrated Samples.

AU - Cartman, Michelle L.

AU - Morris, James A.

AU - Oates, Kenneth

AU - Huddart, Henry

AU - Staff, William G.

PY - 1994

Y1 - 1994

N2 - Samples of neoplastic and nonneoplastic human urothelium were immediately frozen, incubated in Krebs' saline and then frozen, or incubated in 10-5 mol/L ouabain in Krebs' saline and then frozen. The frozen specimens were then planed in a cryoultramicrotome and examined by low-temperature scanning electron microscopy. X-Ray microanalysis was performed on the superficial urothelial cells. Neoplastic cells immediately frozen and those incubated in Krebs' saline had significantly higher K+/Na+, K+/P, and K+/Cl- ratios and lower Na+/P and Cl-/P ratios than nonneoplastic cells. Incubation in ouabain led to a fall in the K+/Na+, K+/P, and K+/Cl- ratios and a rise in the Na+/P and Na+/Cl- ratios in both neoplastic and nonneoplastic cells and effectively nullified the difference between them. These results are consistent with the concept that in neoplasia a primary event is stimulation of Na+/H+ exchange, which leads to secondary stimulation of the ouabain-sensitive Na+/K+ ATPase pump.

AB - Samples of neoplastic and nonneoplastic human urothelium were immediately frozen, incubated in Krebs' saline and then frozen, or incubated in 10-5 mol/L ouabain in Krebs' saline and then frozen. The frozen specimens were then planed in a cryoultramicrotome and examined by low-temperature scanning electron microscopy. X-Ray microanalysis was performed on the superficial urothelial cells. Neoplastic cells immediately frozen and those incubated in Krebs' saline had significantly higher K+/Na+, K+/P, and K+/Cl- ratios and lower Na+/P and Cl-/P ratios than nonneoplastic cells. Incubation in ouabain led to a fall in the K+/Na+, K+/P, and K+/Cl- ratios and a rise in the Na+/P and Na+/Cl- ratios in both neoplastic and nonneoplastic cells and effectively nullified the difference between them. These results are consistent with the concept that in neoplasia a primary event is stimulation of Na+/H+ exchange, which leads to secondary stimulation of the ouabain-sensitive Na+/K+ ATPase pump.

KW - low-temperature scanning electron microscopy

KW - ouabain

KW - quantitative X-ray microanalysis

KW - urothelial tumors

U2 - 10.3109/01913129409023220

DO - 10.3109/01913129409023220

M3 - Journal article

VL - 18

SP - 461

EP - 466

JO - Ultrastructural Pathology

JF - Ultrastructural Pathology

SN - 1521-0758

IS - 5

ER -

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