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Electrospun polyurethane/hydroxyapatite bioactive scaffolds for bone tissue engineering: The role of solvent and hydroxyapatite particles

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Electrospun polyurethane/hydroxyapatite bioactive scaffolds for bone tissue engineering : The role of solvent and hydroxyapatite particles. / Tetteh, G.; Khan, A.S.; Delaine-Smith, R.M.; Reilly, G.C.; Rehman, I.U.

In: Journal of the Mechanical Behavior of Biomedical Materials, Vol. 39, 2014, p. 95-110.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Tetteh, G, Khan, AS, Delaine-Smith, RM, Reilly, GC & Rehman, IU 2014, 'Electrospun polyurethane/hydroxyapatite bioactive scaffolds for bone tissue engineering: The role of solvent and hydroxyapatite particles', Journal of the Mechanical Behavior of Biomedical Materials, vol. 39, pp. 95-110. https://doi.org/10.1016/j.jmbbm.2014.06.019

APA

Tetteh, G., Khan, A. S., Delaine-Smith, R. M., Reilly, G. C., & Rehman, I. U. (2014). Electrospun polyurethane/hydroxyapatite bioactive scaffolds for bone tissue engineering: The role of solvent and hydroxyapatite particles. Journal of the Mechanical Behavior of Biomedical Materials, 39, 95-110. https://doi.org/10.1016/j.jmbbm.2014.06.019

Vancouver

Author

Tetteh, G. ; Khan, A.S. ; Delaine-Smith, R.M. ; Reilly, G.C. ; Rehman, I.U. / Electrospun polyurethane/hydroxyapatite bioactive scaffolds for bone tissue engineering : The role of solvent and hydroxyapatite particles. In: Journal of the Mechanical Behavior of Biomedical Materials. 2014 ; Vol. 39. pp. 95-110.

Bibtex

@article{93fdd8e4e9154adca83513785d707213,
title = "Electrospun polyurethane/hydroxyapatite bioactive scaffolds for bone tissue engineering: The role of solvent and hydroxyapatite particles",
abstract = "Polyurethane (PU) is a promising polymer to support bone-matrix producing cells due to its durability and mechanical resistance. In this study two types of medical grade poly-ether urethanes Z3A1 and Z9A1 and PU-Hydroxyapatite (PU-HA) composites were investigated for their ability to act as a scaffold for tissue engineered bone. PU dissolved in varying concentrations of dimethylformamide (DMF) and tetrahydrofuran (THF) solvents were electrospun to attain scaffolds with randomly orientated non-woven fibres. Bioactive polymeric composite scaffolds were created using 15. wt% Z3A1 in a 70/30 DMF/THF PU solution and incorporating micro- or nano-sized HA particles in a ratio of 3:1 respectively, whilst a 25. wt% Z9A1 PU solution was doped in ratio of 5:1. Chemical properties of the resulting composites were evaluated by FTIR and physical properties by SEM. Tensile mechanical testing was carried out on all electrospun scaffolds. MLO-A5 osteoblastic mouse cells and human embryonic mesenchymal progenitor cells, hES-MPs were seeded on the scaffolds to test their biocompatibility and ability to support mineralised matrix production over a 28 day culture period. Cell viability was assayed by MTT and calcium and collagen deposition by Sirius red and alizarin red respectively. SEM images of both electrospun PU scaffolds and PU-HA composite scaffolds showed differences in fibre morphology with changes in solvent combinations and size of HA particles. Inclusion of THF eliminated the presence of beads in fibres that were present in scaffolds fabricated with 100% DMF solvent, and resulted in fibres with a more uniform morphology and thicker diameters. Mechanical testing demonstrated that the Young[U+05F3]s Modulus and yield strength was lower at higher THF concentrations. Inclusion of both sizes of HA particles in PU-HA solutions reinforced the scaffolds leading to higher mechanical properties, whilst FTIR characterisation confirmed the presence of HA in all composite scaffolds. Although all scaffolds supported proliferation of both cell types and deposition of calcified matrix, PU-HA composite fibres containing nano-HA enabled the highest cell viability and collagen deposition. These scaffolds have the potential to support bone matrix formation for bone tissue engineering. {\textcopyright} 2014 The Authors.",
keywords = "Electrospinning, FTIR characterisation, Hydroxyapatite, Mesenchymal stem cell, Osteoblast, Polyurethane, Biocompatibility, Biomechanics, Bone, Cell culture, Cells, Collagen, Deposition, Fibers, Mechanical properties, Mechanical testing, Morphology, Organic solvents, Osteoblasts, Particle reinforced composites, Polyurethanes, Solvents, Cytology, Elastic moduli, Polymers, Stem cells, Tensile testing, Tissue, Tissue engineering, Bone tissue engineering, FTIR, Hydroxyapatite particles, Mesenchymal progenitor cells, Tensile mechanical testing, Tetrahydrofuran solvents, Tissue-engineered bones, Scaffolds (biology), alizarin, calcium, collagen, hydroxyapatite, n,n dimethylformamide, nanoparticle, polyurethan, solvent, tetrahydrofuran, tissue scaffold, urethan, alizarin red s, furan derivative, animal cell, article, bone matrix, bone tissue, cell culture, cell type, cell viability, dissolution, electrospinning, Fourier transform infrared photoacoustic spectroscopy, human, human cell, mesenchymal stem cell, morphology, mouse, nonhuman, osteoblast, physical chemistry, polymerization, priority journal, scanning electron microscopy, tensile strength, tissue engineering, Young modulus, Article, biocompatibility, controlled study, embryo, MTT assay, particle size, animal, bone, cell survival, chemistry, cytology, infrared spectroscopy, materials testing, mechanical stress, mesenchymal stroma cell, metabolism, pathology, procedures, Animals, Bone and Bones, Cell Survival, Dimethylformamide, Durapatite, Furans, Humans, Materials Testing, Mesenchymal Stromal Cells, Mice, Microscopy, Electron, Scanning, Spectroscopy, Fourier Transform Infrared, Stress, Mechanical, Tensile Strength, Tissue Engineering, Tissue Scaffolds",
author = "G. Tetteh and A.S. Khan and R.M. Delaine-Smith and G.C. Reilly and I.U. Rehman",
year = "2014",
doi = "10.1016/j.jmbbm.2014.06.019",
language = "English",
volume = "39",
pages = "95--110",
journal = "Journal of the Mechanical Behavior of Biomedical Materials",
issn = "1751-6161",
publisher = "Elsevier Ltd",

}

RIS

TY - JOUR

T1 - Electrospun polyurethane/hydroxyapatite bioactive scaffolds for bone tissue engineering

T2 - The role of solvent and hydroxyapatite particles

AU - Tetteh, G.

AU - Khan, A.S.

AU - Delaine-Smith, R.M.

AU - Reilly, G.C.

AU - Rehman, I.U.

PY - 2014

Y1 - 2014

N2 - Polyurethane (PU) is a promising polymer to support bone-matrix producing cells due to its durability and mechanical resistance. In this study two types of medical grade poly-ether urethanes Z3A1 and Z9A1 and PU-Hydroxyapatite (PU-HA) composites were investigated for their ability to act as a scaffold for tissue engineered bone. PU dissolved in varying concentrations of dimethylformamide (DMF) and tetrahydrofuran (THF) solvents were electrospun to attain scaffolds with randomly orientated non-woven fibres. Bioactive polymeric composite scaffolds were created using 15. wt% Z3A1 in a 70/30 DMF/THF PU solution and incorporating micro- or nano-sized HA particles in a ratio of 3:1 respectively, whilst a 25. wt% Z9A1 PU solution was doped in ratio of 5:1. Chemical properties of the resulting composites were evaluated by FTIR and physical properties by SEM. Tensile mechanical testing was carried out on all electrospun scaffolds. MLO-A5 osteoblastic mouse cells and human embryonic mesenchymal progenitor cells, hES-MPs were seeded on the scaffolds to test their biocompatibility and ability to support mineralised matrix production over a 28 day culture period. Cell viability was assayed by MTT and calcium and collagen deposition by Sirius red and alizarin red respectively. SEM images of both electrospun PU scaffolds and PU-HA composite scaffolds showed differences in fibre morphology with changes in solvent combinations and size of HA particles. Inclusion of THF eliminated the presence of beads in fibres that were present in scaffolds fabricated with 100% DMF solvent, and resulted in fibres with a more uniform morphology and thicker diameters. Mechanical testing demonstrated that the Young[U+05F3]s Modulus and yield strength was lower at higher THF concentrations. Inclusion of both sizes of HA particles in PU-HA solutions reinforced the scaffolds leading to higher mechanical properties, whilst FTIR characterisation confirmed the presence of HA in all composite scaffolds. Although all scaffolds supported proliferation of both cell types and deposition of calcified matrix, PU-HA composite fibres containing nano-HA enabled the highest cell viability and collagen deposition. These scaffolds have the potential to support bone matrix formation for bone tissue engineering. © 2014 The Authors.

AB - Polyurethane (PU) is a promising polymer to support bone-matrix producing cells due to its durability and mechanical resistance. In this study two types of medical grade poly-ether urethanes Z3A1 and Z9A1 and PU-Hydroxyapatite (PU-HA) composites were investigated for their ability to act as a scaffold for tissue engineered bone. PU dissolved in varying concentrations of dimethylformamide (DMF) and tetrahydrofuran (THF) solvents were electrospun to attain scaffolds with randomly orientated non-woven fibres. Bioactive polymeric composite scaffolds were created using 15. wt% Z3A1 in a 70/30 DMF/THF PU solution and incorporating micro- or nano-sized HA particles in a ratio of 3:1 respectively, whilst a 25. wt% Z9A1 PU solution was doped in ratio of 5:1. Chemical properties of the resulting composites were evaluated by FTIR and physical properties by SEM. Tensile mechanical testing was carried out on all electrospun scaffolds. MLO-A5 osteoblastic mouse cells and human embryonic mesenchymal progenitor cells, hES-MPs were seeded on the scaffolds to test their biocompatibility and ability to support mineralised matrix production over a 28 day culture period. Cell viability was assayed by MTT and calcium and collagen deposition by Sirius red and alizarin red respectively. SEM images of both electrospun PU scaffolds and PU-HA composite scaffolds showed differences in fibre morphology with changes in solvent combinations and size of HA particles. Inclusion of THF eliminated the presence of beads in fibres that were present in scaffolds fabricated with 100% DMF solvent, and resulted in fibres with a more uniform morphology and thicker diameters. Mechanical testing demonstrated that the Young[U+05F3]s Modulus and yield strength was lower at higher THF concentrations. Inclusion of both sizes of HA particles in PU-HA solutions reinforced the scaffolds leading to higher mechanical properties, whilst FTIR characterisation confirmed the presence of HA in all composite scaffolds. Although all scaffolds supported proliferation of both cell types and deposition of calcified matrix, PU-HA composite fibres containing nano-HA enabled the highest cell viability and collagen deposition. These scaffolds have the potential to support bone matrix formation for bone tissue engineering. © 2014 The Authors.

KW - Electrospinning

KW - FTIR characterisation

KW - Hydroxyapatite

KW - Mesenchymal stem cell

KW - Osteoblast

KW - Polyurethane

KW - Biocompatibility

KW - Biomechanics

KW - Bone

KW - Cell culture

KW - Cells

KW - Collagen

KW - Deposition

KW - Fibers

KW - Mechanical properties

KW - Mechanical testing

KW - Morphology

KW - Organic solvents

KW - Osteoblasts

KW - Particle reinforced composites

KW - Polyurethanes

KW - Solvents

KW - Cytology

KW - Elastic moduli

KW - Polymers

KW - Stem cells

KW - Tensile testing

KW - Tissue

KW - Tissue engineering

KW - Bone tissue engineering

KW - FTIR

KW - Hydroxyapatite particles

KW - Mesenchymal progenitor cells

KW - Tensile mechanical testing

KW - Tetrahydrofuran solvents

KW - Tissue-engineered bones

KW - Scaffolds (biology)

KW - alizarin

KW - calcium

KW - collagen

KW - hydroxyapatite

KW - n,n dimethylformamide

KW - nanoparticle

KW - polyurethan

KW - solvent

KW - tetrahydrofuran

KW - tissue scaffold

KW - urethan

KW - alizarin red s

KW - furan derivative

KW - animal cell

KW - article

KW - bone matrix

KW - bone tissue

KW - cell culture

KW - cell type

KW - cell viability

KW - dissolution

KW - electrospinning

KW - Fourier transform infrared photoacoustic spectroscopy

KW - human

KW - human cell

KW - mesenchymal stem cell

KW - morphology

KW - mouse

KW - nonhuman

KW - osteoblast

KW - physical chemistry

KW - polymerization

KW - priority journal

KW - scanning electron microscopy

KW - tensile strength

KW - tissue engineering

KW - Young modulus

KW - Article

KW - biocompatibility

KW - controlled study

KW - embryo

KW - MTT assay

KW - particle size

KW - animal

KW - bone

KW - cell survival

KW - chemistry

KW - cytology

KW - infrared spectroscopy

KW - materials testing

KW - mechanical stress

KW - mesenchymal stroma cell

KW - metabolism

KW - pathology

KW - procedures

KW - Animals

KW - Bone and Bones

KW - Cell Survival

KW - Dimethylformamide

KW - Durapatite

KW - Furans

KW - Humans

KW - Materials Testing

KW - Mesenchymal Stromal Cells

KW - Mice

KW - Microscopy, Electron, Scanning

KW - Spectroscopy, Fourier Transform Infrared

KW - Stress, Mechanical

KW - Tensile Strength

KW - Tissue Engineering

KW - Tissue Scaffolds

U2 - 10.1016/j.jmbbm.2014.06.019

DO - 10.1016/j.jmbbm.2014.06.019

M3 - Journal article

VL - 39

SP - 95

EP - 110

JO - Journal of the Mechanical Behavior of Biomedical Materials

JF - Journal of the Mechanical Behavior of Biomedical Materials

SN - 1751-6161

ER -