Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Encapsulation of the peptide Ac-Glu-Thr-Lys-Thr-Tyr-Phe-Trp-Lys-NH 2 into polyvinyl alcohol biodegradable formulations
T2 - effect of calcium alginate
AU - Patsialas, Konstantinos
AU - Papaioannou, Emmanouil H.
AU - Liakopoulou-Kyriakides, Maria
PY - 2012/1/15
Y1 - 2012/1/15
N2 - It has been recently reported that the peptide Ac-Glu-Thr-Lys-Thr-Tyr-Phe- Trp-Lys-NH2, analogue of the Glu1811-Lys1818 region of A3 light chain of blood coagulation factor VIII, presents in vitro significant anticoagulant activity. The encapsulation of this peptide into different polyvinyl alcohol formulations is examined here. The formulations were prepared using polyvinyl alcohol cross-linked with either boric acid or glutaraldehyde, giving a series of twelve different hydrogels. In case of PVA-boric acid method, a small percentage of sodium alginate was used in order to avoid bead's agglomeration. In that case, the most efficient encapsulation of the octapeptide (74%) was achieved with 0.2% (w/w) sodium alginate. It was also observed that the increase in sodium alginate percentage leads to beads with increased peptide release time, ranging from 60 to 90 min at 0.02% and 1% (w/w) sodium alginate respectively. The water holding of the PVA gels was estimated to be 27% regardless of the cross-linking reagent used, while it was increased with increasing sodium alginate concentration and reached about 60% for 1% sodium alginate. The longer octapeptide release, at 120 min, was observed with PVA-glutaraldehyde hydrogel, with encapsulation efficiency comparable to those obtained with boric acid, indicating that this hydrogel may be further used in drug delivery systems.
AB - It has been recently reported that the peptide Ac-Glu-Thr-Lys-Thr-Tyr-Phe- Trp-Lys-NH2, analogue of the Glu1811-Lys1818 region of A3 light chain of blood coagulation factor VIII, presents in vitro significant anticoagulant activity. The encapsulation of this peptide into different polyvinyl alcohol formulations is examined here. The formulations were prepared using polyvinyl alcohol cross-linked with either boric acid or glutaraldehyde, giving a series of twelve different hydrogels. In case of PVA-boric acid method, a small percentage of sodium alginate was used in order to avoid bead's agglomeration. In that case, the most efficient encapsulation of the octapeptide (74%) was achieved with 0.2% (w/w) sodium alginate. It was also observed that the increase in sodium alginate percentage leads to beads with increased peptide release time, ranging from 60 to 90 min at 0.02% and 1% (w/w) sodium alginate respectively. The water holding of the PVA gels was estimated to be 27% regardless of the cross-linking reagent used, while it was increased with increasing sodium alginate concentration and reached about 60% for 1% sodium alginate. The longer octapeptide release, at 120 min, was observed with PVA-glutaraldehyde hydrogel, with encapsulation efficiency comparable to those obtained with boric acid, indicating that this hydrogel may be further used in drug delivery systems.
KW - Biodegradable polymer
KW - Encapsulation
KW - Factor VIII
KW - Polyvinyl alcohol
KW - Synthetic peptide
U2 - 10.1016/j.carbpol.2011.08.064
DO - 10.1016/j.carbpol.2011.08.064
M3 - Journal article
AN - SCOPUS:81255134466
VL - 87
SP - 1112
EP - 1118
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
SN - 0144-8617
IS - 2
ER -