Home > Research > Publications & Outputs > Endogenously inhibited protein kinase C in tran...
View graph of relations

Endogenously inhibited protein kinase C in transgenic Drosophila embryonic neuroblasts down regulates the outgrowth of type I and II processes of cultured mature neurons

Research output: Contribution to journalJournal articlepeer-review

Published

Standard

Endogenously inhibited protein kinase C in transgenic Drosophila embryonic neuroblasts down regulates the outgrowth of type I and II processes of cultured mature neurons. / Broughton, S J; Kane, N S; Arthur, B; Yoder, M; Greenspan, R J; Robichon, A.

In: Journal of Cellular Biochemistry, Vol. 60, No. 4, 15.03.1996, p. 584-599.

Research output: Contribution to journalJournal articlepeer-review

Harvard

APA

Vancouver

Author

Broughton, S J ; Kane, N S ; Arthur, B ; Yoder, M ; Greenspan, R J ; Robichon, A. / Endogenously inhibited protein kinase C in transgenic Drosophila embryonic neuroblasts down regulates the outgrowth of type I and II processes of cultured mature neurons. In: Journal of Cellular Biochemistry. 1996 ; Vol. 60, No. 4. pp. 584-599.

Bibtex

@article{e6489c6ed8b14778b04ae95b0976963c,
title = "Endogenously inhibited protein kinase C in transgenic Drosophila embryonic neuroblasts down regulates the outgrowth of type I and II processes of cultured mature neurons",
abstract = "Embryonic neurons were cultured from transgenic Drosophila melanogaster expressing a highly specific pseudosubstrate inhibitor of protein kinase C (PKC). Flies homozygous for this transgene, which is under the control of the yeast UAS promoter, were crossed to flies homozygous for the yeast heat shock inducible transcription factor GAL 4. Following heat shock, the progeny express the pseudosubstrate inhibitor at high levels. This strategy, which has the advantage of avoiding the non-specific effects of drugs, was used to study the role of PKC in process growth of cultured, differentiating neuroblasts. An external gold particle labeling procedure using a cell surface antigen expressed by mature neurons and processes was used to visualize neuronal processes directly in the scanning electron microscope. We observed that cell cultures expressing a low concentration of the pseudosubstrate inhibitor showed a significant decrease in the number of type I and II processes as compared to control cultures, while the proportions of neuroblasts, ganglion mother cells (GMCs), and mature neurons in the clusters were little affected.",
keywords = "Amino Acid Sequence, Animals, Animals, Genetically Modified, Calcium-Calmodulin-Dependent Protein Kinases, Cell Aging, Cells, Cultured, Cyclic AMP-Dependent Protein Kinases, Down-Regulation, Drosophila melanogaster, Enzyme Inhibitors, Molecular Sequence Data, Neurons, Phosphorylation, Protein Kinase C, Stem Cells",
author = "Broughton, {S J} and Kane, {N S} and B Arthur and M Yoder and Greenspan, {R J} and A Robichon",
year = "1996",
month = mar,
day = "15",
doi = "10.1002/(SICI)1097-4644(19960315)60:4<584::AID-JCB14>3.0.CO;2-H",
language = "English",
volume = "60",
pages = "584--599",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "4",

}

RIS

TY - JOUR

T1 - Endogenously inhibited protein kinase C in transgenic Drosophila embryonic neuroblasts down regulates the outgrowth of type I and II processes of cultured mature neurons

AU - Broughton, S J

AU - Kane, N S

AU - Arthur, B

AU - Yoder, M

AU - Greenspan, R J

AU - Robichon, A

PY - 1996/3/15

Y1 - 1996/3/15

N2 - Embryonic neurons were cultured from transgenic Drosophila melanogaster expressing a highly specific pseudosubstrate inhibitor of protein kinase C (PKC). Flies homozygous for this transgene, which is under the control of the yeast UAS promoter, were crossed to flies homozygous for the yeast heat shock inducible transcription factor GAL 4. Following heat shock, the progeny express the pseudosubstrate inhibitor at high levels. This strategy, which has the advantage of avoiding the non-specific effects of drugs, was used to study the role of PKC in process growth of cultured, differentiating neuroblasts. An external gold particle labeling procedure using a cell surface antigen expressed by mature neurons and processes was used to visualize neuronal processes directly in the scanning electron microscope. We observed that cell cultures expressing a low concentration of the pseudosubstrate inhibitor showed a significant decrease in the number of type I and II processes as compared to control cultures, while the proportions of neuroblasts, ganglion mother cells (GMCs), and mature neurons in the clusters were little affected.

AB - Embryonic neurons were cultured from transgenic Drosophila melanogaster expressing a highly specific pseudosubstrate inhibitor of protein kinase C (PKC). Flies homozygous for this transgene, which is under the control of the yeast UAS promoter, were crossed to flies homozygous for the yeast heat shock inducible transcription factor GAL 4. Following heat shock, the progeny express the pseudosubstrate inhibitor at high levels. This strategy, which has the advantage of avoiding the non-specific effects of drugs, was used to study the role of PKC in process growth of cultured, differentiating neuroblasts. An external gold particle labeling procedure using a cell surface antigen expressed by mature neurons and processes was used to visualize neuronal processes directly in the scanning electron microscope. We observed that cell cultures expressing a low concentration of the pseudosubstrate inhibitor showed a significant decrease in the number of type I and II processes as compared to control cultures, while the proportions of neuroblasts, ganglion mother cells (GMCs), and mature neurons in the clusters were little affected.

KW - Amino Acid Sequence

KW - Animals

KW - Animals, Genetically Modified

KW - Calcium-Calmodulin-Dependent Protein Kinases

KW - Cell Aging

KW - Cells, Cultured

KW - Cyclic AMP-Dependent Protein Kinases

KW - Down-Regulation

KW - Drosophila melanogaster

KW - Enzyme Inhibitors

KW - Molecular Sequence Data

KW - Neurons

KW - Phosphorylation

KW - Protein Kinase C

KW - Stem Cells

U2 - 10.1002/(SICI)1097-4644(19960315)60:4<584::AID-JCB14>3.0.CO;2-H

DO - 10.1002/(SICI)1097-4644(19960315)60:4<584::AID-JCB14>3.0.CO;2-H

M3 - Journal article

C2 - 8707897

VL - 60

SP - 584

EP - 599

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 4

ER -