Home > Research > Publications & Outputs > Enhanced biotransformations and product recover...

Research

Links

Text available via DOI:

Keywords

View graph of relations

Enhanced biotransformations and product recovery in a membrane bioreactor through application of a direct electric current.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

Enhanced biotransformations and product recovery in a membrane bioreactor through application of a direct electric current. / Mustacchi, Roberta; Knowles, Christopher J.; Li, Hong et al.
In: Biotechnology and Bioengineering, Vol. 89, No. 1, 05.01.2005, p. 18-23.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Mustacchi, R, Knowles, CJ, Li, H, Dalrymple, I, Sunderland, G, Skibar, W & Jackman, SA 2005, 'Enhanced biotransformations and product recovery in a membrane bioreactor through application of a direct electric current.', Biotechnology and Bioengineering, vol. 89, no. 1, pp. 18-23. https://doi.org/10.1002/bit.20299

APA

Mustacchi, R., Knowles, C. J., Li, H., Dalrymple, I., Sunderland, G., Skibar, W., & Jackman, S. A. (2005). Enhanced biotransformations and product recovery in a membrane bioreactor through application of a direct electric current. Biotechnology and Bioengineering, 89(1), 18-23. https://doi.org/10.1002/bit.20299

Vancouver

Mustacchi R, Knowles CJ, Li H, Dalrymple I, Sunderland G, Skibar W et al. Enhanced biotransformations and product recovery in a membrane bioreactor through application of a direct electric current. Biotechnology and Bioengineering. 2005 Jan 5;89(1):18-23. doi: 10.1002/bit.20299

Author

Mustacchi, Roberta ; Knowles, Christopher J. ; Li, Hong et al. / Enhanced biotransformations and product recovery in a membrane bioreactor through application of a direct electric current. In: Biotechnology and Bioengineering. 2005 ; Vol. 89, No. 1. pp. 18-23.

Bibtex

@article{635228087c264736896d74ba85129d66,
title = "Enhanced biotransformations and product recovery in a membrane bioreactor through application of a direct electric current.",
abstract = "The simultaneous enhancement of biotransformation coupled to product recovery, purification and concentration is presented. The nitrilase of Rhodococcus rhodochrous LL100-21 catalyses the single-step hydrolytic biotransformation of benzonitrile to benzoic acid and ammonia. When a direct electric current is applied across a bioreactor containing the bacterium and benzonitrile, the charged product (benzoic acid) can be removed in situ across an anion exchange membrane and recovered in a separate compartment. Over the course of a 24-hour biotransformation, benzonitrile was converted to benzoic acid which was completely removed from the bioreactor chamber and concentrated 3-fold in a separate chamber. The rate of production of benzoic acid increased by 42% when the current was applied (0.044 mmol/min/g dry cell weight in the presence of current as compared to 0.03 mmol/min/g dry cell weight in its absence). The enhanced reaction rate was achieved irrespective of product separation and therefore appears to be a direct effect upon the bacterial cells. This process has potential for enhanced productivity from biotransformations through a simultaneous increase in metabolic activity and in situ product recovery.",
keywords = "electrokinetics • direct current • nitriles • biotransformations • in situ product recovery",
author = "Roberta Mustacchi and Knowles, {Christopher J.} and Hong Li and Ian Dalrymple and Garry Sunderland and Wolfgang Skibar and Jackman, {Simon A.}",
note = "Funded by: the United Kingdom Department of Trade and Industry and the Biotechnology and Biological Sciences Research Council",
year = "2005",
month = jan,
day = "5",
doi = "10.1002/bit.20299",
language = "English",
volume = "89",
pages = "18--23",
journal = "Biotechnology and Bioengineering",
issn = "1097-0290",
publisher = "Wiley-VCH Verlag",
number = "1",

}

RIS

TY - JOUR

T1 - Enhanced biotransformations and product recovery in a membrane bioreactor through application of a direct electric current.

AU - Mustacchi, Roberta

AU - Knowles, Christopher J.

AU - Li, Hong

AU - Dalrymple, Ian

AU - Sunderland, Garry

AU - Skibar, Wolfgang

AU - Jackman, Simon A.

N1 - Funded by: the United Kingdom Department of Trade and Industry and the Biotechnology and Biological Sciences Research Council

PY - 2005/1/5

Y1 - 2005/1/5

N2 - The simultaneous enhancement of biotransformation coupled to product recovery, purification and concentration is presented. The nitrilase of Rhodococcus rhodochrous LL100-21 catalyses the single-step hydrolytic biotransformation of benzonitrile to benzoic acid and ammonia. When a direct electric current is applied across a bioreactor containing the bacterium and benzonitrile, the charged product (benzoic acid) can be removed in situ across an anion exchange membrane and recovered in a separate compartment. Over the course of a 24-hour biotransformation, benzonitrile was converted to benzoic acid which was completely removed from the bioreactor chamber and concentrated 3-fold in a separate chamber. The rate of production of benzoic acid increased by 42% when the current was applied (0.044 mmol/min/g dry cell weight in the presence of current as compared to 0.03 mmol/min/g dry cell weight in its absence). The enhanced reaction rate was achieved irrespective of product separation and therefore appears to be a direct effect upon the bacterial cells. This process has potential for enhanced productivity from biotransformations through a simultaneous increase in metabolic activity and in situ product recovery.

AB - The simultaneous enhancement of biotransformation coupled to product recovery, purification and concentration is presented. The nitrilase of Rhodococcus rhodochrous LL100-21 catalyses the single-step hydrolytic biotransformation of benzonitrile to benzoic acid and ammonia. When a direct electric current is applied across a bioreactor containing the bacterium and benzonitrile, the charged product (benzoic acid) can be removed in situ across an anion exchange membrane and recovered in a separate compartment. Over the course of a 24-hour biotransformation, benzonitrile was converted to benzoic acid which was completely removed from the bioreactor chamber and concentrated 3-fold in a separate chamber. The rate of production of benzoic acid increased by 42% when the current was applied (0.044 mmol/min/g dry cell weight in the presence of current as compared to 0.03 mmol/min/g dry cell weight in its absence). The enhanced reaction rate was achieved irrespective of product separation and therefore appears to be a direct effect upon the bacterial cells. This process has potential for enhanced productivity from biotransformations through a simultaneous increase in metabolic activity and in situ product recovery.

KW - electrokinetics • direct current • nitriles • biotransformations • in situ product recovery

U2 - 10.1002/bit.20299

DO - 10.1002/bit.20299

M3 - Journal article

VL - 89

SP - 18

EP - 23

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 1097-0290

IS - 1

ER -