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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Enhancement of biomimetic enzymatic mineralization of gellan gum polysaccharide hydrogels by plant-derived gallotannins
AU - Douglas, Timothy
AU - Keppler, Julia K.
AU - Vandrovcova, Marta
AU - Plnencer, Martin
AU - Beranova, Jana
AU - Feuereisen, Michelle
AU - Parakhonskiy, Bogdan V
AU - Svenskaya, Yulia
AU - Atkin, Vsevolod
AU - Ivanova, Anna
AU - Ricquier , Patrick
AU - Balcaen, Lieve
AU - Vanhaecke, Frank
AU - Schieber, Andreas
AU - Bacakova, Lucie
AU - Skirtach, Andre G.
PY - 2020/3/27
Y1 - 2020/3/27
N2 - Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration. Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols. In this study, ALP-loaded gellan gum (GG) hydrogels were enriched with gallotannins, a subclass of polyphenols. Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.). Certain gallotannin preparations promoted mineralization to a greater degree than others. The various gallotannin preparations bound differently to ALP and influenced the size of aggregates of ALP, which may be related to ability to promote mineralization. Human osteoblast-like Saos-2 cells grew in eluate from mineralized hydrogels. Gallotannin incorporation impeded cell growth on hydrogels and did not impart antibacterial activity. In conclusion, gallotannin incorporation aided mineralization but reduced cytocompatibility.
AB - Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration. Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols. In this study, ALP-loaded gellan gum (GG) hydrogels were enriched with gallotannins, a subclass of polyphenols. Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.). Certain gallotannin preparations promoted mineralization to a greater degree than others. The various gallotannin preparations bound differently to ALP and influenced the size of aggregates of ALP, which may be related to ability to promote mineralization. Human osteoblast-like Saos-2 cells grew in eluate from mineralized hydrogels. Gallotannin incorporation impeded cell growth on hydrogels and did not impart antibacterial activity. In conclusion, gallotannin incorporation aided mineralization but reduced cytocompatibility.
KW - mineralization
KW - polyphenol
KW - composite
KW - protein-polyphenol interaction
KW - gellan gum
KW - enzyme
U2 - 10.3390/ijms21072315
DO - 10.3390/ijms21072315
M3 - Journal article
VL - 21
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1422-0067
IS - 7
M1 - 2315
ER -