Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol(®) to endow antibacterial properties and promote mineralization

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol(®) to endow antibacterial properties and promote mineralization. / Douglas, Timothy E L; Dokupil, Agnieszka; Reczyńska, Katarzyna et al.
In: Biomedical Materials, Vol. 11, No. 4, 045015, 10.08.2016.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Douglas, TEL, Dokupil, A, Reczyńska, K, Brackman, G, Krok-Borkowicz, M, Keppler, JK, Božič, M, Van Der Voort, P, Pietryga, K, Samal, SK, Balcaen, L, van den Bulcke, J, Van Acker, J, Vanhaecke, F, Schwarz, K, Coenye, T & Pamuła, E 2016, 'Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol(®) to endow antibacterial properties and promote mineralization', Biomedical Materials, vol. 11, no. 4, 045015. https://doi.org/10.1088/1748-6041/11/4/045015

APA

Douglas, T. E. L., Dokupil, A., Reczyńska, K., Brackman, G., Krok-Borkowicz, M., Keppler, J. K., Božič, M., Van Der Voort, P., Pietryga, K., Samal, S. K., Balcaen, L., van den Bulcke, J., Van Acker, J., Vanhaecke, F., Schwarz, K., Coenye, T., & Pamuła, E. (2016). Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol(®) to endow antibacterial properties and promote mineralization. Biomedical Materials, 11(4), Article 045015. https://doi.org/10.1088/1748-6041/11/4/045015

Vancouver

Douglas TEL, Dokupil A, Reczyńska K, Brackman G, Krok-Borkowicz M, Keppler JK et al. Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol(®) to endow antibacterial properties and promote mineralization. Biomedical Materials. 2016 Aug 10;11(4):045015. doi: 10.1088/1748-6041/11/4/045015

Author

Douglas, Timothy E L ; Dokupil, Agnieszka ; Reczyńska, Katarzyna et al. / Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol(®) to endow antibacterial properties and promote mineralization. In: Biomedical Materials. 2016 ; Vol. 11, No. 4.

Bibtex

@article{5a518ecbfee6446cb29441565ab517dc,

title = "Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol({\textregistered}) to endow antibacterial properties and promote mineralization",

abstract = "Hydrogels offer several advantages as biomaterials for bone regeneration, including ease of incorporation of soluble substances such as mineralization-promoting enzymes and antibacterial agents. Mineralization with calcium phosphate (CaP) increases bioactivity, while antibacterial activity reduces the risk of infection. Here, gellan gum (GG) hydrogels were enriched with alkaline phosphatase (ALP) and/or Seanol({\textregistered}), a seaweed extract rich in phlorotannins (brown algae-derived polyphenols), to induce mineralization with CaP and increase antibacterial activity, respectively. The sample groups were unmineralized hydrogels, denoted as GG, GG/ALP, GG/Seanol and GG/Seanol/ALP, and hydrogels incubated in mineralization medium (0.1 M calcium glycerophosphate), denoted as GG/ALP_min, GG/Seanol_min and GG/Seanol/ALP_min. Seanol({\textregistered}) enhanced mineralization with CaP and also increased compressive modulus. Seanol({\textregistered}) and ALP interacted in a non-covalent manner. Release of Seanol({\textregistered}) occurred in a burst phase and was impeded by ALP-mediated mineralization. Groups GG/Seanol and GG/ALP/Seanol exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus. GG/Seanol/ALP_min, but not GG/Seanol_min, retained some antibacterial activity. Eluates taken from groups GG/ALP_min, GG/Seanol_min and GG/ALP/Seanol_min displayed comparable cytotoxicity towards MG-63 osteoblast-like cells. These results suggest that enrichment of hydrogel biomaterials with phlorotannin-rich extracts is a promising strategy to increase mineralizability and antibacterial activity.",

keywords = "Alkaline Phosphatase/metabolism, Anti-Bacterial Agents/chemistry, Anti-Infective Agents/chemistry, Biocompatible Materials/chemistry, Bone Regeneration, Calcium Phosphates/chemistry, Cell Line, Humans, Hydrogels/chemistry, Methicillin-Resistant Staphylococcus aureus, Microbial Sensitivity Tests, Osteoblasts/cytology, Polysaccharides, Bacterial/chemistry, Seaweed/chemistry, Solubility, Spectroscopy, Fourier Transform Infrared, Stress, Mechanical, Tannins/chemistry, Thermogravimetry",

author = "Douglas, {Timothy E L} and Agnieszka Dokupil and Katarzyna Reczy{\'n}ska and Gilles Brackman and Malgorzata Krok-Borkowicz and Keppler, {Julia K} and Mojca Bo{\v z}i{\v c} and {Van Der Voort}, Pascal and Krzysztof Pietryga and Samal, {Sangram Keshari} and Lieve Balcaen and {van den Bulcke}, Jan and {Van Acker}, Joris and Frank Vanhaecke and Karin Schwarz and Tom Coenye and El{\.z}bieta Pamu{\l}a",

year = "2016",

month = aug,

day = "10",

doi = "10.1088/1748-6041/11/4/045015",

language = "English",

volume = "11",

journal = "Biomedical Materials",

issn = "1748-6041",

publisher = "IOP Science",

number = "4",

}

RIS

TY - JOUR

T1 - Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol(®) to endow antibacterial properties and promote mineralization

AU - Douglas, Timothy E L

AU - Dokupil, Agnieszka

AU - Reczyńska, Katarzyna

AU - Brackman, Gilles

AU - Krok-Borkowicz, Malgorzata

AU - Keppler, Julia K

AU - Božič, Mojca

AU - Van Der Voort, Pascal

AU - Pietryga, Krzysztof

AU - Samal, Sangram Keshari

AU - Balcaen, Lieve

AU - van den Bulcke, Jan

AU - Van Acker, Joris

AU - Vanhaecke, Frank

AU - Schwarz, Karin

AU - Coenye, Tom

AU - Pamuła, Elżbieta

PY - 2016/8/10

Y1 - 2016/8/10

N2 - Hydrogels offer several advantages as biomaterials for bone regeneration, including ease of incorporation of soluble substances such as mineralization-promoting enzymes and antibacterial agents. Mineralization with calcium phosphate (CaP) increases bioactivity, while antibacterial activity reduces the risk of infection. Here, gellan gum (GG) hydrogels were enriched with alkaline phosphatase (ALP) and/or Seanol(®), a seaweed extract rich in phlorotannins (brown algae-derived polyphenols), to induce mineralization with CaP and increase antibacterial activity, respectively. The sample groups were unmineralized hydrogels, denoted as GG, GG/ALP, GG/Seanol and GG/Seanol/ALP, and hydrogels incubated in mineralization medium (0.1 M calcium glycerophosphate), denoted as GG/ALP_min, GG/Seanol_min and GG/Seanol/ALP_min. Seanol(®) enhanced mineralization with CaP and also increased compressive modulus. Seanol(®) and ALP interacted in a non-covalent manner. Release of Seanol(®) occurred in a burst phase and was impeded by ALP-mediated mineralization. Groups GG/Seanol and GG/ALP/Seanol exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus. GG/Seanol/ALP_min, but not GG/Seanol_min, retained some antibacterial activity. Eluates taken from groups GG/ALP_min, GG/Seanol_min and GG/ALP/Seanol_min displayed comparable cytotoxicity towards MG-63 osteoblast-like cells. These results suggest that enrichment of hydrogel biomaterials with phlorotannin-rich extracts is a promising strategy to increase mineralizability and antibacterial activity.

AB - Hydrogels offer several advantages as biomaterials for bone regeneration, including ease of incorporation of soluble substances such as mineralization-promoting enzymes and antibacterial agents. Mineralization with calcium phosphate (CaP) increases bioactivity, while antibacterial activity reduces the risk of infection. Here, gellan gum (GG) hydrogels were enriched with alkaline phosphatase (ALP) and/or Seanol(®), a seaweed extract rich in phlorotannins (brown algae-derived polyphenols), to induce mineralization with CaP and increase antibacterial activity, respectively. The sample groups were unmineralized hydrogels, denoted as GG, GG/ALP, GG/Seanol and GG/Seanol/ALP, and hydrogels incubated in mineralization medium (0.1 M calcium glycerophosphate), denoted as GG/ALP_min, GG/Seanol_min and GG/Seanol/ALP_min. Seanol(®) enhanced mineralization with CaP and also increased compressive modulus. Seanol(®) and ALP interacted in a non-covalent manner. Release of Seanol(®) occurred in a burst phase and was impeded by ALP-mediated mineralization. Groups GG/Seanol and GG/ALP/Seanol exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus. GG/Seanol/ALP_min, but not GG/Seanol_min, retained some antibacterial activity. Eluates taken from groups GG/ALP_min, GG/Seanol_min and GG/ALP/Seanol_min displayed comparable cytotoxicity towards MG-63 osteoblast-like cells. These results suggest that enrichment of hydrogel biomaterials with phlorotannin-rich extracts is a promising strategy to increase mineralizability and antibacterial activity.

KW - Alkaline Phosphatase/metabolism

KW - Anti-Bacterial Agents/chemistry

KW - Anti-Infective Agents/chemistry

KW - Biocompatible Materials/chemistry

KW - Bone Regeneration

KW - Calcium Phosphates/chemistry

KW - Cell Line

KW - Humans

KW - Hydrogels/chemistry

KW - Methicillin-Resistant Staphylococcus aureus

KW - Microbial Sensitivity Tests

KW - Osteoblasts/cytology

KW - Polysaccharides, Bacterial/chemistry

KW - Seaweed/chemistry

KW - Solubility

KW - Spectroscopy, Fourier Transform Infrared

KW - Stress, Mechanical

KW - Tannins/chemistry

KW - Thermogravimetry

U2 - 10.1088/1748-6041/11/4/045015

DO - 10.1088/1748-6041/11/4/045015

M3 - Journal article

C2 - 27509486

VL - 11

JO - Biomedical Materials

JF - Biomedical Materials

SN - 1748-6041

IS - 4

M1 - 045015

ER -

Research

Links

Text available via DOI:

Keywords