Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase

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Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase. / Douglas, Timothy E.L.; Messersmith, Philip B.; Chasan, Safak et al.
In: Macromolecular Bioscience, Vol. 12, No. 8, 08.2012, p. 1077-1089.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Douglas, TEL, Messersmith, PB, Chasan, S, Mikos, AG, de Mulder, ELW, Dickson, G, Schaubroeck, D, Balcaen, L, Vanhaecke, F, Dubruel, P, Jansen, JA & Leeuwenburgh, SCG 2012, 'Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase', Macromolecular Bioscience, vol. 12, no. 8, pp. 1077-1089. https://doi.org/10.1002/mabi.201100501

APA

Douglas, T. E. L., Messersmith, P. B., Chasan, S., Mikos, A. G., de Mulder, E. L. W., Dickson, G., Schaubroeck, D., Balcaen, L., Vanhaecke, F., Dubruel, P., Jansen, J. A., & Leeuwenburgh, S. C. G. (2012). Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase. Macromolecular Bioscience, 12(8), 1077-1089. https://doi.org/10.1002/mabi.201100501

Vancouver

Douglas TEL, Messersmith PB, Chasan S, Mikos AG, de Mulder ELW, Dickson G et al. Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase. Macromolecular Bioscience. 2012 Aug;12(8):1077-1089. Epub 2012 May 30. doi: 10.1002/mabi.201100501

Author

Douglas, Timothy E.L. ; Messersmith, Philip B. ; Chasan, Safak et al. / Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase. In: Macromolecular Bioscience. 2012 ; Vol. 12, No. 8. pp. 1077-1089.

Bibtex

@article{d9b1c96ced5745f0bc576d711a783f46,

title = "Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase",

abstract = "Alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, is incorporated into three hydrogel biomaterials to induce their mineralization with calcium phosphate (CaP). These are collagen type I, a mussel-protein-inspired adhesive consisting of PEG substituted with catechol groups, cPEG, and the PEG/fumaric acid copolymer OPF. After incubation in Ca-GP solution, FTIR, EDS, SEM, XRD, SAED, ICP-OES, and von Kossa staining confirm CaP formation. The amount of mineral formed decreases in the order cPEG>collagen>OPF. The mineral:polymer ratio decreases in the order collagen>cPEG>OPF. Mineralization increases Young's modulus, most profoundly for cPEG. Such enzymatically mineralized hydrogel/CaP composites may find application as bone regeneration materials. Enzymatic mineralization of three hydrogel biomaterials with calcium phosphate (CaP) is achieved by functionalization with alkaline phosphatase (ALP). Characterization of the hydrogels collagen type I, cPEG, and OPF reveals different degrees of mineralization, suggesting the possibility of enhancing mineralization for bone tissue engineering by the choice of hydrogel.",

keywords = "Biomaterials, Biomineralization, Composites, Enzymes, Hydrogels",

author = "Douglas, {Timothy E.L.} and Messersmith, {Philip B.} and Safak Chasan and Mikos, {Antonios G.} and {de Mulder}, {Eric L.W.} and Glenn Dickson and David Schaubroeck and Lieve Balcaen and Frank Vanhaecke and Peter Dubruel and Jansen, {John A.} and Leeuwenburgh, {Sander C.G.}",

year = "2012",

month = aug,

doi = "10.1002/mabi.201100501",

language = "English",

volume = "12",

pages = "1077--1089",

journal = "Macromolecular Bioscience",

issn = "1616-5187",

publisher = "Wiley-VCH Verlag",

number = "8",

}

RIS

TY - JOUR

T1 - Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase

AU - Douglas, Timothy E.L.

AU - Messersmith, Philip B.

AU - Chasan, Safak

AU - Mikos, Antonios G.

AU - de Mulder, Eric L.W.

AU - Dickson, Glenn

AU - Schaubroeck, David

AU - Balcaen, Lieve

AU - Vanhaecke, Frank

AU - Dubruel, Peter

AU - Jansen, John A.

AU - Leeuwenburgh, Sander C.G.

PY - 2012/8

Y1 - 2012/8

N2 - Alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, is incorporated into three hydrogel biomaterials to induce their mineralization with calcium phosphate (CaP). These are collagen type I, a mussel-protein-inspired adhesive consisting of PEG substituted with catechol groups, cPEG, and the PEG/fumaric acid copolymer OPF. After incubation in Ca-GP solution, FTIR, EDS, SEM, XRD, SAED, ICP-OES, and von Kossa staining confirm CaP formation. The amount of mineral formed decreases in the order cPEG>collagen>OPF. The mineral:polymer ratio decreases in the order collagen>cPEG>OPF. Mineralization increases Young's modulus, most profoundly for cPEG. Such enzymatically mineralized hydrogel/CaP composites may find application as bone regeneration materials. Enzymatic mineralization of three hydrogel biomaterials with calcium phosphate (CaP) is achieved by functionalization with alkaline phosphatase (ALP). Characterization of the hydrogels collagen type I, cPEG, and OPF reveals different degrees of mineralization, suggesting the possibility of enhancing mineralization for bone tissue engineering by the choice of hydrogel.

AB - Alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, is incorporated into three hydrogel biomaterials to induce their mineralization with calcium phosphate (CaP). These are collagen type I, a mussel-protein-inspired adhesive consisting of PEG substituted with catechol groups, cPEG, and the PEG/fumaric acid copolymer OPF. After incubation in Ca-GP solution, FTIR, EDS, SEM, XRD, SAED, ICP-OES, and von Kossa staining confirm CaP formation. The amount of mineral formed decreases in the order cPEG>collagen>OPF. The mineral:polymer ratio decreases in the order collagen>cPEG>OPF. Mineralization increases Young's modulus, most profoundly for cPEG. Such enzymatically mineralized hydrogel/CaP composites may find application as bone regeneration materials. Enzymatic mineralization of three hydrogel biomaterials with calcium phosphate (CaP) is achieved by functionalization with alkaline phosphatase (ALP). Characterization of the hydrogels collagen type I, cPEG, and OPF reveals different degrees of mineralization, suggesting the possibility of enhancing mineralization for bone tissue engineering by the choice of hydrogel.

KW - Biomaterials

KW - Biomineralization

KW - Composites

KW - Enzymes

KW - Hydrogels

U2 - 10.1002/mabi.201100501

DO - 10.1002/mabi.201100501

M3 - Journal article

C2 - 22648976

AN - SCOPUS:84864824027

VL - 12

SP - 1077

EP - 1089

JO - Macromolecular Bioscience

JF - Macromolecular Bioscience

SN - 1616-5187

IS - 8

ER -

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