Home > Research > Publications & Outputs > Enzymatically biomineralized chitosan scaffolds...

Links

Text available via DOI:

View graph of relations

Enzymatically biomineralized chitosan scaffolds for tissue-engineering applications

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

Enzymatically biomineralized chitosan scaffolds for tissue-engineering applications. / Dash, Mamoni; Samal, Sangram K.; Douglas, Timothy E. L.; Schaubroeck, David; Leeuwenburgh, Sander C.; Voort, Pascal Van Der; Declercq, Heidi A.; Dubruel, Peter.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 11, No. 5, 01.05.2017, p. 1500-1513.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Dash, M, Samal, SK, Douglas, TEL, Schaubroeck, D, Leeuwenburgh, SC, Voort, PVD, Declercq, HA & Dubruel, P 2017, 'Enzymatically biomineralized chitosan scaffolds for tissue-engineering applications', Journal of Tissue Engineering and Regenerative Medicine, vol. 11, no. 5, pp. 1500-1513. https://doi.org/10.1002/term.2048

APA

Dash, M., Samal, S. K., Douglas, T. E. L., Schaubroeck, D., Leeuwenburgh, S. C., Voort, P. V. D., Declercq, H. A., & Dubruel, P. (2017). Enzymatically biomineralized chitosan scaffolds for tissue-engineering applications. Journal of Tissue Engineering and Regenerative Medicine, 11(5), 1500-1513. https://doi.org/10.1002/term.2048

Vancouver

Dash M, Samal SK, Douglas TEL, Schaubroeck D, Leeuwenburgh SC, Voort PVD et al. Enzymatically biomineralized chitosan scaffolds for tissue-engineering applications. Journal of Tissue Engineering and Regenerative Medicine. 2017 May 1;11(5):1500-1513. https://doi.org/10.1002/term.2048

Author

Dash, Mamoni ; Samal, Sangram K. ; Douglas, Timothy E. L. ; Schaubroeck, David ; Leeuwenburgh, Sander C. ; Voort, Pascal Van Der ; Declercq, Heidi A. ; Dubruel, Peter. / Enzymatically biomineralized chitosan scaffolds for tissue-engineering applications. In: Journal of Tissue Engineering and Regenerative Medicine. 2017 ; Vol. 11, No. 5. pp. 1500-1513.

Bibtex

@article{ac575567409746f99d5b65233884add6,
title = "Enzymatically biomineralized chitosan scaffolds for tissue-engineering applications",
abstract = "Porous biodegradable scaffolds represent promising candidates for tissue‐engineering applications because of their capability to be preseeded with cells. We report an uncrosslinked chitosan scaffold designed with the aim of inducing and supporting enzyme‐mediated formation of apatite minerals in the absence of osteogenic growth factors. To realize this, natural enzyme alkaline phosphatase (ALP) was incorporated into uncrosslinked chitosan scaffolds. The uncrosslinked chitosan makes available amine and alcohol functionalities to enhance the biomineralization process. The physicochemical findings revealed homogeneous mineralization, with the phase structure of the formed minerals resembling that of apatite at low mineral concentrations, and similar to dicalcium phosphate dihydrate (DCPD) with increasing ALP content. The MC3T3 cell activity clearly showed that the mineralization of the chitosan scaffolds was effective in improving cellular adhesion, proliferation and colonization. ",
author = "Mamoni Dash and Samal, {Sangram K.} and Douglas, {Timothy E. L.} and David Schaubroeck and Leeuwenburgh, {Sander C.} and Voort, {Pascal Van Der} and Declercq, {Heidi A.} and Peter Dubruel",
year = "2017",
month = may,
day = "1",
doi = "10.1002/term.2048",
language = "English",
volume = "11",
pages = "1500--1513",
journal = "Journal of Tissue Engineering and Regenerative Medicine",
issn = "1932-6254",
publisher = "John Wiley and Sons Ltd",
number = "5",

}

RIS

TY - JOUR

T1 - Enzymatically biomineralized chitosan scaffolds for tissue-engineering applications

AU - Dash, Mamoni

AU - Samal, Sangram K.

AU - Douglas, Timothy E. L.

AU - Schaubroeck, David

AU - Leeuwenburgh, Sander C.

AU - Voort, Pascal Van Der

AU - Declercq, Heidi A.

AU - Dubruel, Peter

PY - 2017/5/1

Y1 - 2017/5/1

N2 - Porous biodegradable scaffolds represent promising candidates for tissue‐engineering applications because of their capability to be preseeded with cells. We report an uncrosslinked chitosan scaffold designed with the aim of inducing and supporting enzyme‐mediated formation of apatite minerals in the absence of osteogenic growth factors. To realize this, natural enzyme alkaline phosphatase (ALP) was incorporated into uncrosslinked chitosan scaffolds. The uncrosslinked chitosan makes available amine and alcohol functionalities to enhance the biomineralization process. The physicochemical findings revealed homogeneous mineralization, with the phase structure of the formed minerals resembling that of apatite at low mineral concentrations, and similar to dicalcium phosphate dihydrate (DCPD) with increasing ALP content. The MC3T3 cell activity clearly showed that the mineralization of the chitosan scaffolds was effective in improving cellular adhesion, proliferation and colonization.

AB - Porous biodegradable scaffolds represent promising candidates for tissue‐engineering applications because of their capability to be preseeded with cells. We report an uncrosslinked chitosan scaffold designed with the aim of inducing and supporting enzyme‐mediated formation of apatite minerals in the absence of osteogenic growth factors. To realize this, natural enzyme alkaline phosphatase (ALP) was incorporated into uncrosslinked chitosan scaffolds. The uncrosslinked chitosan makes available amine and alcohol functionalities to enhance the biomineralization process. The physicochemical findings revealed homogeneous mineralization, with the phase structure of the formed minerals resembling that of apatite at low mineral concentrations, and similar to dicalcium phosphate dihydrate (DCPD) with increasing ALP content. The MC3T3 cell activity clearly showed that the mineralization of the chitosan scaffolds was effective in improving cellular adhesion, proliferation and colonization.

U2 - 10.1002/term.2048

DO - 10.1002/term.2048

M3 - Journal article

VL - 11

SP - 1500

EP - 1513

JO - Journal of Tissue Engineering and Regenerative Medicine

JF - Journal of Tissue Engineering and Regenerative Medicine

SN - 1932-6254

IS - 5

ER -