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Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells

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Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells. / Rimmer, Stephen; Johnson, Claire; Zhao, Bojun et al.
In: Biomaterials, Vol. 28, No. 35, 12.2007, p. 5319-5331.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Rimmer, S, Johnson, C, Zhao, B, Collier, J, Gilmore, L, Sabnis, S, Wyman, P, Sammon, C, Fullwood, NJ & MacNeil, S 2007, 'Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells', Biomaterials, vol. 28, no. 35, pp. 5319-5331. https://doi.org/10.1016/j.biomaterials.2007.08.028

APA

Rimmer, S., Johnson, C., Zhao, B., Collier, J., Gilmore, L., Sabnis, S., Wyman, P., Sammon, C., Fullwood, N. J., & MacNeil, S. (2007). Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells. Biomaterials, 28(35), 5319-5331. https://doi.org/10.1016/j.biomaterials.2007.08.028

Vancouver

Rimmer S, Johnson C, Zhao B, Collier J, Gilmore L, Sabnis S et al. Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells. Biomaterials. 2007 Dec;28(35):5319-5331. doi: 10.1016/j.biomaterials.2007.08.028

Author

Rimmer, Stephen ; Johnson, Claire ; Zhao, Bojun et al. / Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells. In: Biomaterials. 2007 ; Vol. 28, No. 35. pp. 5319-5331.

Bibtex

@article{450f1eddf3b64599873bce745eeaf829,
title = "Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells",
abstract = "The aim of this study was to develop a hydrogel which would be suitable for corneal cell re-epithelialization when used as a corneal implant. To achieve this, a series of hydrogels were functionalized with primary amines by post-polymerization reactions between amine compounds and glycidyl ether groups attached to the hydrogels. We report a strong correlation between the structure of the amine and the viability of stromal cells and epithelial cells cultured on these hydrogels. Subsequent co-culture of epithelial and stromal cells on the amine modified hydrogels allowed successful expansion of epithelial cells on surfaces functionalized with alkyl alpha-omega diamines with carbon chain lengths of between 3 and 6. Analysis of variance showed that corneal epithelial cells had a strong preference for surfaces functionalized by the reaction of excess 1,3 diaminopropane with units of glycidyl methacrylate compared to the reaction products of other amines (ammonia; 1,2-diaminoethane; 1,4-diaminobutane or 1,6-diaminohexane). We suggest this approach of amine functionalization combined with stromal/epithelial co-culture offers a promising new approach to achieving a secure corneal epithelium.",
keywords = "hydrogels, amines, cornea, epithelial cells, METHACRYLATE-BLOCK-(2,3 PROPANDIOL-1-METHACRYLATE-STAT-ETHANDIOL DIMETHACRYLATE)), XENOBIOTIC-FREE CULTURE, AMPHIPHILIC NETWORKS, HUMAN KERATINOCYTES, EXTRACELLULAR-MATRIX, DERMAL FIBROBLASTS, BLOCK CONETWORKS, CHITOSAN FILMS, CLINICAL-USE, ADHESION",
author = "Stephen Rimmer and Claire Johnson and Bojun Zhao and Joyleen Collier and Louisa Gilmore and Subodh Sabnis and Paul Wyman and Christopher Sammon and Fullwood, {Nigel J.} and Sheila MacNeil",
year = "2007",
month = dec,
doi = "10.1016/j.biomaterials.2007.08.028",
language = "English",
volume = "28",
pages = "5319--5331",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "PERGAMON-ELSEVIER SCIENCE LTD",
number = "35",

}

RIS

TY - JOUR

T1 - Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells

AU - Rimmer, Stephen

AU - Johnson, Claire

AU - Zhao, Bojun

AU - Collier, Joyleen

AU - Gilmore, Louisa

AU - Sabnis, Subodh

AU - Wyman, Paul

AU - Sammon, Christopher

AU - Fullwood, Nigel J.

AU - MacNeil, Sheila

PY - 2007/12

Y1 - 2007/12

N2 - The aim of this study was to develop a hydrogel which would be suitable for corneal cell re-epithelialization when used as a corneal implant. To achieve this, a series of hydrogels were functionalized with primary amines by post-polymerization reactions between amine compounds and glycidyl ether groups attached to the hydrogels. We report a strong correlation between the structure of the amine and the viability of stromal cells and epithelial cells cultured on these hydrogels. Subsequent co-culture of epithelial and stromal cells on the amine modified hydrogels allowed successful expansion of epithelial cells on surfaces functionalized with alkyl alpha-omega diamines with carbon chain lengths of between 3 and 6. Analysis of variance showed that corneal epithelial cells had a strong preference for surfaces functionalized by the reaction of excess 1,3 diaminopropane with units of glycidyl methacrylate compared to the reaction products of other amines (ammonia; 1,2-diaminoethane; 1,4-diaminobutane or 1,6-diaminohexane). We suggest this approach of amine functionalization combined with stromal/epithelial co-culture offers a promising new approach to achieving a secure corneal epithelium.

AB - The aim of this study was to develop a hydrogel which would be suitable for corneal cell re-epithelialization when used as a corneal implant. To achieve this, a series of hydrogels were functionalized with primary amines by post-polymerization reactions between amine compounds and glycidyl ether groups attached to the hydrogels. We report a strong correlation between the structure of the amine and the viability of stromal cells and epithelial cells cultured on these hydrogels. Subsequent co-culture of epithelial and stromal cells on the amine modified hydrogels allowed successful expansion of epithelial cells on surfaces functionalized with alkyl alpha-omega diamines with carbon chain lengths of between 3 and 6. Analysis of variance showed that corneal epithelial cells had a strong preference for surfaces functionalized by the reaction of excess 1,3 diaminopropane with units of glycidyl methacrylate compared to the reaction products of other amines (ammonia; 1,2-diaminoethane; 1,4-diaminobutane or 1,6-diaminohexane). We suggest this approach of amine functionalization combined with stromal/epithelial co-culture offers a promising new approach to achieving a secure corneal epithelium.

KW - hydrogels

KW - amines

KW - cornea

KW - epithelial cells

KW - METHACRYLATE-BLOCK-(2,3 PROPANDIOL-1-METHACRYLATE-STAT-ETHANDIOL DIMETHACRYLATE))

KW - XENOBIOTIC-FREE CULTURE

KW - AMPHIPHILIC NETWORKS

KW - HUMAN KERATINOCYTES

KW - EXTRACELLULAR-MATRIX

KW - DERMAL FIBROBLASTS

KW - BLOCK CONETWORKS

KW - CHITOSAN FILMS

KW - CLINICAL-USE

KW - ADHESION

U2 - 10.1016/j.biomaterials.2007.08.028

DO - 10.1016/j.biomaterials.2007.08.028

M3 - Journal article

VL - 28

SP - 5319

EP - 5331

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 35

ER -