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Escherichia coli as an antibody expression host for the production of diagnostic proteins: significance and expression

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Escherichia coli as an antibody expression host for the production of diagnostic proteins: significance and expression. / Huleani, S.; Roberts, M.R.; Beales, L. et al.
In: Critical Reviews in Biotechnology, Vol. 42, No. 5, 30.06.2022, p. 756-773.

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Huleani S, Roberts MR, Beales L, Papaioannou EH. Escherichia coli as an antibody expression host for the production of diagnostic proteins: significance and expression. Critical Reviews in Biotechnology. 2022 Jun 30;42(5):756-773. Epub 2021 Sept 1. doi: 10.1080/07388551.2021.1967871

Author

Huleani, S. ; Roberts, M.R. ; Beales, L. et al. / Escherichia coli as an antibody expression host for the production of diagnostic proteins: significance and expression. In: Critical Reviews in Biotechnology. 2022 ; Vol. 42, No. 5. pp. 756-773.

Bibtex

@article{85aa729db8a34f7cb94fde829847281b,
title = "Escherichia coli as an antibody expression host for the production of diagnostic proteins: significance and expression",
abstract = "This review article concerns the production of recombinant antibody fragments for applications mainly in the diagnostic sector. The so-called “point of care diagnostics” is very important for timely diagnosis and treatment, thus being able to save lives and resources. There is intense pressure for more accurate and less expensive rapid diagnostic tests, with a value preferably ",
keywords = "affinity tags, Antibody fragments expression in E. coli, bioreactors, E. coli cultures, expression plasmid, periplasmic antibody fragments expression, scale-up, Cost effectiveness, Escherichia coli, Gene transfer, Recombinant proteins, Antibody expression, Cost-effective production, Culture parameters, Diagnostic tests, Downstream-processing, Point of care diagnostic, Recombinant antibody, Target values, Antibodies",
author = "S. Huleani and M.R. Roberts and L. Beales and E.H. Papaioannou",
year = "2022",
month = jun,
day = "30",
doi = "10.1080/07388551.2021.1967871",
language = "English",
volume = "42",
pages = "756--773",
journal = "Critical Reviews in Biotechnology",
issn = "0738-8551",
publisher = "Taylor & Francis",
number = "5",

}

RIS

TY - JOUR

T1 - Escherichia coli as an antibody expression host for the production of diagnostic proteins: significance and expression

AU - Huleani, S.

AU - Roberts, M.R.

AU - Beales, L.

AU - Papaioannou, E.H.

PY - 2022/6/30

Y1 - 2022/6/30

N2 - This review article concerns the production of recombinant antibody fragments for applications mainly in the diagnostic sector. The so-called “point of care diagnostics” is very important for timely diagnosis and treatment, thus being able to save lives and resources. There is intense pressure for more accurate and less expensive rapid diagnostic tests, with a value preferably

AB - This review article concerns the production of recombinant antibody fragments for applications mainly in the diagnostic sector. The so-called “point of care diagnostics” is very important for timely diagnosis and treatment, thus being able to save lives and resources. There is intense pressure for more accurate and less expensive rapid diagnostic tests, with a value preferably

KW - affinity tags

KW - Antibody fragments expression in E. coli

KW - bioreactors

KW - E. coli cultures

KW - expression plasmid

KW - periplasmic antibody fragments expression

KW - scale-up

KW - Cost effectiveness

KW - Escherichia coli

KW - Gene transfer

KW - Recombinant proteins

KW - Antibody expression

KW - Cost-effective production

KW - Culture parameters

KW - Diagnostic tests

KW - Downstream-processing

KW - Point of care diagnostic

KW - Recombinant antibody

KW - Target values

KW - Antibodies

U2 - 10.1080/07388551.2021.1967871

DO - 10.1080/07388551.2021.1967871

M3 - Journal article

VL - 42

SP - 756

EP - 773

JO - Critical Reviews in Biotechnology

JF - Critical Reviews in Biotechnology

SN - 0738-8551

IS - 5

ER -