Final published version
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Escherichia coli as an antibody expression host for the production of diagnostic proteins: significance and expression
AU - Huleani, S.
AU - Roberts, M.R.
AU - Beales, L.
AU - Papaioannou, E.H.
PY - 2022/6/30
Y1 - 2022/6/30
N2 - This review article concerns the production of recombinant antibody fragments for applications mainly in the diagnostic sector. The so-called “point of care diagnostics” is very important for timely diagnosis and treatment, thus being able to save lives and resources. There is intense pressure for more accurate and less expensive rapid diagnostic tests, with a value preferably
AB - This review article concerns the production of recombinant antibody fragments for applications mainly in the diagnostic sector. The so-called “point of care diagnostics” is very important for timely diagnosis and treatment, thus being able to save lives and resources. There is intense pressure for more accurate and less expensive rapid diagnostic tests, with a value preferably
KW - affinity tags
KW - Antibody fragments expression in E. coli
KW - bioreactors
KW - E. coli cultures
KW - expression plasmid
KW - periplasmic antibody fragments expression
KW - scale-up
KW - Cost effectiveness
KW - Escherichia coli
KW - Gene transfer
KW - Recombinant proteins
KW - Antibody expression
KW - Cost-effective production
KW - Culture parameters
KW - Diagnostic tests
KW - Downstream-processing
KW - Point of care diagnostic
KW - Recombinant antibody
KW - Target values
KW - Antibodies
U2 - 10.1080/07388551.2021.1967871
DO - 10.1080/07388551.2021.1967871
M3 - Journal article
VL - 42
SP - 756
EP - 773
JO - Critical Reviews in Biotechnology
JF - Critical Reviews in Biotechnology
SN - 0738-8551
IS - 5
ER -